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NMR Methods for Studying Enzyme Kinetics in Cells and Tissue

  • K. M. Brindle
  • I. D. Campbell
  • R. J. Simpson

Abstract

Defining the role of an enzyme in the control of cellular metabolism by extrapolating kinetic data obtained with the isolated enzyme in vitro to the intact cell assumes some knowledge of the intracellular environment. While such an extrapolation has been valuable in constructing models of metabolic control, this may not always be valid. It is important therefore to be able to study an enzyme in an intact system.

Keywords

Creatine Kinase Magnetization Transfer Adenylate Kinase Exchange Velocity Saturation Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alger, J. R., den Hollander, J. A., and Shulman, R. G., 1982, Biochemistry 21:2957CrossRefGoogle Scholar
  2. Alger, J., and Shulman, R.G., 1984, Q. Rev. Biophys. 17:83.CrossRefGoogle Scholar
  3. Alger, J. R., Sillerud, L. O., Behar, K. L., Gillies, R. G., Shulman, R. G., Gordon, R.E., Shaw, D., and Hanley, P.E., 1981, Science 214:660.CrossRefGoogle Scholar
  4. Arágon, J. J., Feliu, J. E., Frenkel, R. A., and Sols, A., 1980, Proc. Natl. Acad. Sci. U.S.A. 77:6324.CrossRefGoogle Scholar
  5. Ashley, D. L., and Goldstein, J. H., 1981, J. Membr. Biol. 61:199.CrossRefGoogle Scholar
  6. Bailey, I. A., Gadian, D. G., Matthews, P. M., Radda, G. K., and Seeley, P. J., 1981, FEBS Lett. 123:315.CrossRefGoogle Scholar
  7. Balaban, R. S., and Ferretti, J. A., 1983, Proc. Natl. Acad. Sci. U.S.A. 80:1241.CrossRefGoogle Scholar
  8. Balaban, R. S., Kantor, H. L., and Ferretti, J. A., 1983, J. Biol. Chem. 258:12787.Google Scholar
  9. Bauer, C., Freeman, R., Frenkiel, T., Keeler, J., and Shaka, A. J., 1984, J. Magn. Reson. 58:442.Google Scholar
  10. Bendall, M. R., Pegg, D. T., Doddrell, D. M., and Field, J., 1981, J. Am. Chem. Soc. 103:934.CrossRefGoogle Scholar
  11. Behar, K. L., den Hollander, J. A., Stromski, M. E., Ogino, T., Shulman, R. G., Petroff, O. A. C., and Prichard, J. W., 1983, Proc. Natl. Acad. Sci. U.S.A. 80:4945.CrossRefGoogle Scholar
  12. Bodenhausen, G., and Ernst, R. R., 1982, J. Am. Chem. Soc. 104:1304.CrossRefGoogle Scholar
  13. Boyd, J., Brindle, K. M., Campbell, I. D., and Radda, G. K., J. Magn. Reson. 60:149.Google Scholar
  14. Brindle, K. M., and Campbell, I. D., 1984 in Biomedical Magnetic Resonance (T. L. James and A. R. Margulis, eds.), pp. 243–255, Radiology Research and Education Foundation, San Francisco.Google Scholar
  15. Brindle, K. M., and Radda, G. K., 1985, Biochim. Biophys Acta 829:188.CrossRefGoogle Scholar
  16. Brindle, K. M., Brown, F. F., Campbell, I. D., Grathwohl, C., and Kuchel, P. W., 1979, Biochem. J. 180:37.Google Scholar
  17. Brindle, K. M., Brown, F. F., Campbell, I. D., Foxall, D. L., and Simpson, R. J., 1982a, Biochem. J. 202:589.Google Scholar
  18. Brindle, K. M., CampbeU, I. D., and Simpson, R. J., 1982b Biochem. J. 208:583.Google Scholar
  19. Brindle, K. M., Campbell, I. D., and Simpson, R. J., 1986, Eur. J. Biochem. 158:299.CrossRefGoogle Scholar
  20. Brindle, K. M., Boyd, J., Campbell, I. D., Porteous, R., and Soffe, N., 1982c, Biochem. Biophys. Res. Commun. 109:864.CrossRefGoogle Scholar
  21. Brindle, K. M., Porteous, R., and Campbell, I. D., 1984a, J. Magn. Reson. 56:543.Google Scholar
  22. Brindle, K. M., Porteous, R. and Radda, G. K., 1984b, Biochim Biophys. Acta 786:18.CrossRefGoogle Scholar
  23. Brown, T. R., 1980, Philos. Trans. R. Soc. London Ser. B 289:441.CrossRefGoogle Scholar
  24. Brown, T. R., and Ogawa, S., 1977, Proc. Natl. Acad. Sci. U.S.A. 74:3627.CrossRefGoogle Scholar
  25. Brown, T. R., Ugurbil, K., and Shulman, R. G., 1977, Proc. Natl. Acad. Sci. U.S.A. 74:5551.CrossRefGoogle Scholar
  26. Brown, T. R., Gadian, D.G., Gariick, P. B., Radda, G. K., Seeley, P. J., and Styles, P., 1978, Frontiers Biol. Energ. 2:1341.Google Scholar
  27. Bücher, Th., 1969, in Pyridine Nucleotide Dependent Dehydrogenases (H. Sund, ed.), pp. 439–461, Springer-Verlag, Berlin.Google Scholar
  28. CampbeU, I. D., Dobson, C. M., Ratcliffe, R. G., and Williams, R. J. P., 1978, J. Magn. Reson. 29:397.Google Scholar
  29. Chance, E.M., Seeholzer, S. H., Koybayashi, K., and Williamson, J. R., 1983, J. Biol. Chem. 258:13785.Google Scholar
  30. Cleland, W. W., 1967, Ann. Rev. Biochem. 36:77.CrossRefGoogle Scholar
  31. Cleland, W. W., 1980, Methods Enzymol. 64:104.CrossRefGoogle Scholar
  32. Cohen, S. M., 1983 J. Biol. Chem. 258:14294.Google Scholar
  33. Cohen, S. M., Rognstad, R., Shulman, R. G., and Katz, J., 1981 J. Biol. Chem. 256:3428.Google Scholar
  34. Conn, M., 1982, Ann. Rev. Biophys. Bioeng. 11:23.CrossRefGoogle Scholar
  35. Conlon, T., and Outhred, R., 1971, Biochem. Biophys. Acta 288:354.Google Scholar
  36. Cooper, A. J. L., 1976, J. Biol. Chem. 251:1088.Google Scholar
  37. Crout, D.H. G., Lutsdorf, M., and Morgan, P. J., 1983, Tetrahedron 39:3457.CrossRefGoogle Scholar
  38. Degani, H., Laughlin, M. R., Campbell, S. L., Ogino, T., and Shulman, R. G., 1984, Third Annual Meeting of Society of Magnetic Resonance in Medicine, New York, p. 185.Google Scholar
  39. den Hollander, J. A., Brown, T. R., Ugurbil, K., and Shulman, R. G., 1979, Proc. Natl. Acad. Sci. U.S.A. 76:6096.CrossRefGoogle Scholar
  40. Dobson, C. M., Olejniczak, E.T., Poulsen, F. M., and Ratcliffe, R. G., 1982, J. Magn. Reson. 48:97.Google Scholar
  41. Doddrell, D. M., Nicholls, K. M., and Sanders, J. K. M., 1984, FEBS Lett. 170:73.CrossRefGoogle Scholar
  42. Doussiere, J., Ligeti, E., Brandolin, G., and Vignais, P. V., 1984, Biochim. Biophys. Acta 766:492.CrossRefGoogle Scholar
  43. Forsén, S., and Hoffman, R. A., 1963, J. Chem. Phys. 39:2892.CrossRefGoogle Scholar
  44. Forsén, S., and Hoffman, R. A., 1964, J. Chem. Phys. 40:1189.CrossRefGoogle Scholar
  45. Foxall, D. L., Brindle, K. M., Campbell, I. D., and Simpson, R. J., 1983a, Tetrahedron 39:3443.CrossRefGoogle Scholar
  46. Foxall, D.L., Cohen, J. S., and Tschudin, R. G., 1983b, J. Magn. Reson. 51:330.Google Scholar
  47. Foxall, D. L., Brindle, K. M., Campbell, I. D., and Simpson, R. J., 1984, Biochim. Biophys. Acta 804:209.CrossRefGoogle Scholar
  48. Freeman, R., Mareci, T. H., and Morris, G. A., 1981, J. Magn. Reson. 42:341.Google Scholar
  49. Gadian, D.G., 1982, NMR of Living Systems ,Oxford University Press.Google Scholar
  50. Gadian, D. G., 1983, Ann. Rev. Biophys. Bioeng. 12:69.CrossRefGoogle Scholar
  51. Gadian, D. G., and Radda, G. K., 1981, Ann. Rev. Biochem. 50:69.CrossRefGoogle Scholar
  52. Gadian, D. G., Radda, G. K., Brown, T. R., Chance, E.M., Dawson, M. J., and Wilkie, D. R., 1981, Biochem. J. 194:215.Google Scholar
  53. Garlick, P. B., Radda, G. K., and Seeley, P. J., 1979, Biochem. J. 184:547.Google Scholar
  54. Gupta, R. K., 1979, Biochim. Biophys. Acta 586:189.Google Scholar
  55. Gupta, R. K., and Gupta, P., 1982, J. Magn. Reson. 47:344.Google Scholar
  56. Hore, P. J., 1983, J. Magn. Reson. 54:539.Google Scholar
  57. Hoult, D. I., 1976, J. Magn. Reson. 21:337.Google Scholar
  58. Hunter, B. K., Nicholls, K. M., and Sanders, J. K. M., 1984, Biochemistry 23:508.CrossRefGoogle Scholar
  59. Iles, R. A., Stevens, A. N., and Griffiths, J. R. 1982, Prog. NMR Spectrosc. 15:49.CrossRefGoogle Scholar
  60. Jardetzky, O., and Roberts, G. C. K., 1981, NMR in Molecular Biology ,Academic, New York.Google Scholar
  61. Jeener, J., Meier, B. H., Bachman, P., and Ernst, R. R., 1979, J. Chem. Phys. 71:4546.CrossRefGoogle Scholar
  62. Ji, S., Lemasters, J. J., and Thurman, R. G., 1980, FEBS Lett. 113:37.CrossRefGoogle Scholar
  63. Kacser, H., and Burns, J. A., 1979, Biochem. Soc. Trans 7:1149.Google Scholar
  64. Kantor, H. L., Feretti, J. A., and Balaban, R. S., 1984, Biochim. Biophys. Acta 789:128.CrossRefGoogle Scholar
  65. Kohen, E., Kohen, C., Thoreil, B., and Bartick, P., 1979, Exp. Cell. Res. 119:23.CrossRefGoogle Scholar
  66. Koretsky, A. P., and Weiner, M. W., 1984, in Biomedical Magnetic Resonance (T. L. James and A. R. Margulis, eds.), pp. 209–230, Radiology Research and Education Foundation, San Francisco.Google Scholar
  67. Kuchel, P. W., and Chapman, B. E., 1984, J. Theor. Biol. 105:569.CrossRefGoogle Scholar
  68. Kupriyanov, V. V., Steinschneider, A. Ya., Ruuge, E. K., Kapel’ko, V. I., Zueva, M. Yu., Lakomkin, V. L., Smiraov, V. N., and Saks, V. A., 1984, Biochim. Biophys. Acta 805:319.CrossRefGoogle Scholar
  69. Masters, C. J., 1977, Curr. Top. Cell. Regul. 12:75.Google Scholar
  70. Matthews, P. M., Bland, J. L., Gadian, D. G., and Radda, G. K., 1981, Biochem. Biophys. Res. Commun. 103:1052.CrossRefGoogle Scholar
  71. Matthews, P. M., Bland, J. L., Gadian, D. G., and Radda, G. K., 1982, Biochim. Biophys. Acta 721:312.CrossRefGoogle Scholar
  72. Matwiyoff, N. A., and Needham, T. E., 1972, Biochem. Biophys. Res. Commun. 49:1158.CrossRefGoogle Scholar
  73. McConnell, H. M., 1958, J. Chem. Phys. 28:430.CrossRefGoogle Scholar
  74. Meyer, R. A., Kushmerick, M. J., and Brown, T. R., 1982, Am. J. Physiol. 11:C1.Google Scholar
  75. Misawa, K., Lee, T. M., and Ogawa, S., 1982, Biochim. Biophys. Acta 718:227.Google Scholar
  76. Morrison, G. A., and Freeman, R., 1978, J. Magn. Reson. 29:433.Google Scholar
  77. Morrison, J. F., and Cleland, W. W., 1966, J. Biol. Chem. 241:673.Google Scholar
  78. Northrop, D. B., 1977, in Isotope Effects on Enzyme Catalysed Reactions (W. W. Cleland, M. H. O’Leary, and D. B. Northrop, eds.), pp. 122–152, University Park Press, Baltimore.Google Scholar
  79. Nunnally, R. L., and Bottomley, P. A., 1981, Science 211:177.CrossRefGoogle Scholar
  80. Nunnally, R. L., and Hollis, D. P., 1979, Biochemistry 18:3642.CrossRefGoogle Scholar
  81. Ogino, T., Arata, Y., and Fujiwara, S., 1980, Biochemistry 19:3684.CrossRefGoogle Scholar
  82. Oxley, S. T., PLPorteous, R., Brindle, K. M., Boyd, J., and Campbell, I. D., 1984, Biochim. Biophys. Acta 805:19.CrossRefGoogle Scholar
  83. Patterson, A., Jr., and Ettinger, R., 1960, Z. Elektrochem 64:98.Google Scholar
  84. Paul, H-H, Brindle, K. M., Campbell, I. D., and Smith, D. J., 1983, FEBS Lett. 163:185.CrossRefGoogle Scholar
  85. Roberts, J. K. M., and Jardetzky, O., 1981, Biochim. Biophys. Acta 639:53.Google Scholar
  86. Roberts, J. K. M., Wemmer, D., and Jardetzky, O., 1984, Plant Physiol. 74:632.CrossRefGoogle Scholar
  87. Robinson, G., Chapman, B. E., and Kuchel, P. W., 1984, Eur. J. Biochem. 143:643.CrossRefGoogle Scholar
  88. Ross, B. D., Radda, G. K., Gadian, D. G., Rocker, G., Esiri, M., and Falconer-Smith, J., 1981, N. England J. Med. 304:1338.CrossRefGoogle Scholar
  89. Scott, A. I., and Baxter, R. L., 1981, Ann. Rev. Biophys. Bioeng. 10:151.CrossRefGoogle Scholar
  90. Shoubridge, E. A., Briggs, R. W., and Radda, G. K., 1982, FEBS Lett. 140:288.CrossRefGoogle Scholar
  91. Shoubridge, E. A., Bland, J. L., and Radda, G. K., 1984, Biochim. Biophys. Acta 805:72.CrossRefGoogle Scholar
  92. Shulman, R. G., Brown, T. R., Ugurbil, K., Ogawa, S., Cohen, S. M., and den Hollander, J. A., 1979, Science 205:160.CrossRefGoogle Scholar
  93. Sies, H., 1980, Trends in Biochem. Sci. 5:182.CrossRefGoogle Scholar
  94. Sies, H., and Brauser, B., 1980, Methods Biochem. Anal. 26:285.CrossRefGoogle Scholar
  95. Sülerud, L. O., Alger, J. R., and Shulman, R. G., 1981, J. Magn. Reson. 45:142.Google Scholar
  96. Süverstein, E., and Boyer, P.D., 1964, J. Biol. Chem. 239:3901.Google Scholar
  97. Simpson, R. J., Brindle, K. M., Brown, F. F., Campbell, I. D., and Foxall, D. L., 1981, Biochem. J. 193:401.Google Scholar
  98. Simpson, R. J., Brindle, K. M., Brown, F. F., Campbell, I. D., and Foxall, D. L., 1982a, Biochem. J. 202:573.Google Scholar
  99. Simpson, R. J., Brindle, K. M., Brown, F. F., Campbell, I. D., and Foxall, D. L., 1982b, Biochem. J. 202:581.Google Scholar
  100. Simpson, R. J., Brindle, K. M., and Campbell, I. D., 1982c, Biochim. Biophys. Acta 721:191.CrossRefGoogle Scholar
  101. Simpson, R. J., Brindle, K. M., and Campbell, I. D., 1983, Biochim. Biophys. Acta 758:187.Google Scholar
  102. Sols, A., and Marco, R., 1970, Curr. Top. Cell. Regul. 2:227.Google Scholar
  103. Sykes, B. D., and Scott, M., 1972, Ann. Rev. Biophys. Bioeng. 1:27.CrossRefGoogle Scholar
  104. Sykes, B. D., and Grimaldi, J. J., 1978, Methods Enzymol. 49:295.CrossRefGoogle Scholar
  105. Taylor, D. J., Bore, P. J., Styles, P., Gadian, D. G., and Radda, G. K., 1983, Mol. Biol. Med. 1:77.Google Scholar
  106. Torrey, H. C., 1949, Phys. Rev. 76:1059.CrossRefGoogle Scholar
  107. Ugurbil, K., Brown, T. R., den Hollander, J. A., Glynn, P., and Shulman, R. G., 1978, Proc. Natl. Acad. Sci. USA. 75:3742.CrossRefGoogle Scholar
  108. Ugurbil, K., 1984, private communication.Google Scholar
  109. Vasavada, K. V., Kaplan, J. I., and Nageswara Rao, B. D., 1984, Biochemistry 23:961.CrossRefGoogle Scholar
  110. Veech, R. L., Lawson, J. W. R., Cornell, N. W., and Krebs, H. A., 1979, J. Biol. Chem. 254:6538.Google Scholar
  111. Walker, T. E., Han, C. H., Kollman, V. H., London, R. E., and Matwiyoff, N. A., 1982, J. Biol. Chem. 257:1189.Google Scholar
  112. Yagil, G., and Hoberman, H. D., 1969, Biochemistry 8:352.CrossRefGoogle Scholar
  113. York, M. J., Kuchel, P. W., Chapman, B. E., and Jones, A. J., 1982, Biochem. J. 207:65.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • K. M. Brindle
    • 1
  • I. D. Campbell
    • 1
  • R. J. Simpson
    • 1
  1. 1.Department of BiochemistryUniversity of OxfordOxfordUK

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