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In Vitro Cultivation of Nerve Cells as a Model for Studies on Nerve Cell—Virus Interactions

  • Richard J. Ziegler

Abstract

During the last decade, nerve tissue culture techniques have been applied to fundamental questions concerning the mechanisms of nervous system development and function. Culture conditions have varied from complex growth media to chemically defined growth media. Research has focused on such diverse topics as (1) electrophysiological properties, (2) neurotransmitter uptake and release, (3) neuropeptide content, (4) influence of trophic factors, (5) neurochemical receptor binding, (6) receptor-mediated cellular activation, i.e., stimulation of cAMP or cGMP synthesis, (7) synapse formation, and (8) axonal transport. This extensive literature forms a basis for future studies of viral effects on neural function.

Keywords

Schwann Cell Neural Tissue Rabies Virus Continuous Cell Line Human Neuroblastoma Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beidler, J. L., Helson, L., and Spengler, B. A., 1973, Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cell in continuous culture, Cancer Res. 33:2643–2652.Google Scholar
  2. Brockes, J. P., Fields, K. L., and Raff, M., 1979, Studies on cultures of rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve, Brain Res. 165:105–108.PubMedCrossRefGoogle Scholar
  3. Crain, S. M. (ed.), 1976, Neurophysiologic Studies in Tissue Cultures ,Raven Press, New York.Google Scholar
  4. Dingledine, R. (ed.), 1984, Brain Slices ,Plenum Press, New York.Google Scholar
  5. Fedoroff, S., and Hertz, L. (eds.), 1977, Cell, Tissue and Organ Cultures in Neurobiology ,Academic Press, New York.Google Scholar
  6. Fischbach, G. D., and Nelson, P. G., 1977, Cell culture in neurobiology, in: Handbook of Physiology-The Nervous System ,Vol. 1 (E. R. Kandel, ed.), American Physiological Society, Bethesda, pp. 719–774.Google Scholar
  7. Fukuda, J., and Kurata, T., 1981, Loss of membrane excitability after herpes simplex virus infection in tissue-cultured nerve cells from adult mammals, Brain Res. 211:235–241.PubMedCrossRefGoogle Scholar
  8. Fukuda, J., Kurata, T., Yamamoto, A., and Yamaguchi, K., 1983, Morphological and physiological studies on cultured nerve cells from guinea pigs infected with herpes simplex virus in vivo, Brain Res. 262:79–89.PubMedCrossRefGoogle Scholar
  9. Giacobini, E. (ed.), 1980, Tissue Culture in Neurobiology ,Raven Press, New York.Google Scholar
  10. Halbach, M., and Koschel, K., 1979, Impairment of hormone dependent signal transfer by chronic SSPE virus infection, J. Gen. Virol. 42:615–619.PubMedCrossRefGoogle Scholar
  11. Harvey, A. L., 1984, The Pharmacology of Nerve and Muscle in Tissue Culture ,Alan R. Liss, New York.Google Scholar
  12. Hertz, L., Juurlink, B. H. J., Fosmark, H., and Schousboe, A., 1981, Methodological appendix: Astroycytes in primary culture, in: Neuroscience Approached through Cell Culture ,Vol. 1 (S. E. Pfeiffer, ed.), CRC Press, Boca Raton, Florida, pp. 175–186.Google Scholar
  13. Kim, S. U., Stern, J., Kim, M. W., and Pleasure, D. E., 1983, Culture of purified rat astrocytes in serum-free media supplemented with mitogen, Brain Res. 274:79–86.PubMedCrossRefGoogle Scholar
  14. Kimhi, J., 1981, Nerve cells in clonal systems, in: Excitable Cells in Tissue Culture (P. G. Nelson and M. Lieberman, eds.), Plenum Press, New York, pp. 173–245.CrossRefGoogle Scholar
  15. Klee, W. A., and Nirenberg, M., 1974, A neuroblastoma x glioma hybrid cell line with morphine receptors, Proc. Natl. Acad. Sci. U.S.A. 71:3474–3477.PubMedCentralPubMedCrossRefGoogle Scholar
  16. Koschel, K., and Halbach, M., 1979, Rabies virus infection selectively impairs membrane receptor functions in neuronal model cells, J. Gen. Virol. 42:627–637.PubMedCrossRefGoogle Scholar
  17. Koschel, K., and Munzel, P., 1984, Inhibition of opiate receptor-mediated signal transmission by rabies virus in persistently infected NG-108-15 mouse neuroblastoma-rat glioma hybrid cells, Proc. Natl. Acad. Sci. U.S.A. 81:950–954.PubMedCentralPubMedCrossRefGoogle Scholar
  18. Kreider, B. W., Messina, A., Doan, H., Kim, S. U., Lisak, R. P., and Pleasure, D., 1981, Enrichment of Schwann cell cultures from neonatal rat sciatic nerve by differential adhesion, Brain Res. 207:433–444.PubMedCrossRefGoogle Scholar
  19. Lumsden, D. E., 1968, Nervous tissue in culture, in: Structure and Function of Nervous Tissue (G. H. Bourne, ed.), Academic Press, New York, pp. 67–140.Google Scholar
  20. Lycke, E., 1985, Viral-induced changes in neural cells, in: Handbook of Neurochemistry ,Vol. 10 (A. Lajtha, ed.), Plenum Press, New York, pp. 509–532.Google Scholar
  21. Lycke, E., and Ziegler, R., 1983, Herpes simplex virus-neuronal cell interactions, in: Advances in Biological Psychiatry ,Vol. 12 (J. Mendlewicz and H. M. van Praag, eds.), S. Karger, Basel, pp. 97–111.Google Scholar
  22. McAllister, R. M., Isaacs, H., Rongey, R., Peer, M., Au, W., Soukup, S. W., and Gardner, M. D., 1977, Establishment of a human medulloblastoma cell line, Int. J. Cancer 20:206–212.PubMedCrossRefGoogle Scholar
  23. Messer, A., 1977, The maintenance and identification of mouse cerebeller granule cells in monolayer culture, Brain Res. 130:1–12.PubMedCrossRefGoogle Scholar
  24. Minna, J. D., Yavelow, J., and Coon, H. G., 1975, Expression of phenotypes in hybrid somatic cell derived from the nervous system, Genetics 798:373–383.Google Scholar
  25. Munzel, P., and Koschel, K., 1982, Alteration in phospholipid methylation and impairment of signal transmission in persistently paramyxovirus-infected C6 rat glioma cells, Proc. Natl. Acad. Sci. U.S.A. 79:3692–3696.PubMedCentralPubMedCrossRefGoogle Scholar
  26. Nelson, P. G., 1975, Nerve and muscle cells in culture, Physiol. Rev. 55:1–61.PubMedGoogle Scholar
  27. Nelson, P. G., and Liberman, M. (eds.), 1981, Excitable Cells in Tissue Culture ,Plenum Press, New York.Google Scholar
  28. Oldstone, M. B. A., Holmstoen, S., and Welch, R. M., 1977, Alterations of acetylcholine enzymes in neuroblastoma cells persistently infected with lymphocytic choriomeningitis virus, J. Cell. Physiol. 91:459–472.PubMedCrossRefGoogle Scholar
  29. Perez-Polo, J. R., Werrbach-Perez, K., and Tiffany-Castiglioni, E., 1979, A human clonal cell line model of differentiating neurons, Dev. Biol. 71:341–353.PubMedCrossRefGoogle Scholar
  30. Pruss, R. M., Bartlett, P. F., Gavrilovic, J., Lisak, R. P., and Rattray, S., 1982, Mitogens for glial cells: A comparison of the response of cultured astrocytes, oligodendrocytes, and Schwann cells, Dev. Brain Res. 2:19–35.CrossRefGoogle Scholar
  31. Rubenstein, R., and Price, R., 1984, Early inhibition of acetylcholinesterase and choline acetyltransferase activity in Herpes simplex virus type 1 infection of PC-12 cells, J. Neurochem. 42:142–150.PubMedCrossRefGoogle Scholar
  32. Sato, G. (ed.), 1973, Tissue Culture of the Nervous System ,Plenum Press, New York.Google Scholar
  33. Seeds, N. W., 1973, Differentiation of aggregating brain cell cultures, in: Tissue Culture of the Nervous System (G. Sato, ed.), Plenum Press, New York, pp. 35–53.CrossRefGoogle Scholar
  34. Seeger, R. C., Rayner, S. E., Banerjee, A., Chung, H., Laug, W. E., Neustein, H. B., and Benedict, W. F., 1977, Morphology, growth, chromosomal pattern, and fibrinolytic activity of two new human neuroblastoma cell lines, Cancer Res. 37:1364–1371.PubMedGoogle Scholar
  35. Sidell, N., and Horn, R., 1985, Properties of human neuroblastoma cells following induction by retinoic acid, in: Advances in Neuroblastoma Research (A. E. Evan, ed.), Alan R. Liss, New York, pp. 39–53.Google Scholar
  36. Sidell, N., Altman, A., Hannsler, M. R., and Seeger, R. G., 1983, Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines, Exp. Cell Res. 148:21–30.PubMedCrossRefGoogle Scholar
  37. Spector, I., 1981, Electrophysiology of clonal nerve cell lines, in: Excitable Cells in Tissue Culture (P. G. Nelson and M. Lieberman, eds.), Plenum Press, New York, pp. 247–277.CrossRefGoogle Scholar
  38. Trapp, B., and Richelson, E., 1980, Usefulness of rotation-mediated aggregating cell cultures, in: Experimental and Clinical Neurotoxicology (P. S. Spencer and H. H. Schaumburg, eds.), Williams & Wilkins, Baltimore, pp. 803–819.Google Scholar
  39. Tsokos, M., Ross, R. A., and Triche, T., 1985, Neuronal, Schwannian, and melanocytic differentiation of human neuroblastoma cells in vitro ,in: Advances in Neuroblastoma Research (A. E. Evan, ed.), Alan R. Liss, New York, pp. 55–68.Google Scholar
  40. Tumilowicz, J. J., Nichols, W. W., Cholon, J. J., and Greene, A. E., 1970, Definition of a continuous cell line derived from neuroblastoma, Cancer Res. 30:2110–2118.PubMedGoogle Scholar
  41. Wood, P. M., 1976, Separation of functional Schwann cells and neurons from peripheral nerve tissue, Brain Res. 115:361–375.PubMedCrossRefGoogle Scholar
  42. Yu, A. C. H., Hertz, E., and Hertz, L., 1984, Alterations in uptake and release rates for GABA, glutamate, and glutamine during biochemical maturation of highly purified cultures of cerebral cortical neurons, J. Neurochem. 42:451–460.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Richard J. Ziegler
    • 1
  1. 1.Department of Virology, Institute of Medical MicrobiologyUniversity of GöteborgGöteborgSweden

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