The Protein Identification Resource (PIR): An On-Line Computer System for the Characterization of Proteins Based on Comparisons with Previously Characterized Protein Sequences
This conference is dedicated to examining new methods for the isolation and characterization of proteins. One extremely effective method for the characterization of a new protein involves the comparison of its amino acid sequence with the sequences of previously determined proteins. Although: this method is not new (but dates back to the early days of protein sequencing methodology), the wealth of information available is only recently being fully appreciated. The rapid increase in the accumulation of sequence data, owing to recombinant DNA technology, has greatly heightened interest in this area and has made large database searching a much more fruitful enterprise. The primary structures of well over 3, 000 proteins containing almost three quarters of a million residues are now known, more than double what was known just 5 years ago.
KeywordsHuman Epidermal Growth Factor Receptor Epidermal Growth Factor Receptor Protein Amino Acid Percentage Sequence Comparison Method Nucleic Acid Database
Unable to display preview. Download preview PDF.
- 1.L. T. Hunt and M. O. Dayhoff, Evolution of chromosomal proteins, in: “Macromolecular Sequences in Systematic and Evolutionary Biology,” M. Goodman, ed., Plenum, New York (1982).Google Scholar
- 3.A. Ullrich, L. Coussens, J. S. Hayflick, T. J. Dull, A. Gray, A. W. Tam, J. Lee, Y. Yarden, T. A. Libermann, J. Schlessinger, J. Downward, E. L. V. Mayes, N. Whittle, M. D. Waterfield, and P. H. Seeburg, Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells, Nature 309: 418 (1984).PubMedCrossRefGoogle Scholar
- 12.M. O. Dayhoff, R. V. Eck, M. A. Chang, and M. R. Sochard, “Atlas of Protein Sequence and Structure 1965,” National Biomedical Research Foundation, Silver Spring, MD (1965).Google Scholar
- 13.R. V. Eck and M. O. Dayhoff, “Atlas of Protein Sequence and Structure 1966,” National Biomedical Research Foundation, Silver Spring, MD (1966).Google Scholar
- 14.M. O. Dayhoff, ed., “Atlas of Protein Sequence and Structure,” Vol. 5, National Biomedical Research Foundation, Washington (1972).Google Scholar
- 15.M. O. Dayhoff, ed., “Atlas of Protein Sequence and Structure,” Vol. 5, Suppl. 3, National Biomedical Research Foundation, Washington (1979).Google Scholar
- 20.M. S. Waterman, General methods of sequence comparison, Bull. Math. Biol. 46: 473 (1984).Google Scholar
- 21.D. Sankoff and J. B. Kruskal, eds., “Time Warps, String Edits, and Macromolecules: The Theory and practice of Sequence Comparison,” Addison-Wesley, Reading, MA (1983).Google Scholar
- 27.W. R. Widger, W. A. Cramer, R. G. Herrmann, and A. Trebst, Sequence homology and structural similarity between cytochrome b of mitochondrial complex III and the chloroplast b6-f complex: Position of the cytochrome b hemes in the membrane, Proc. Nat. Acad. Sci. USA 81: 674 (1984).PubMedCrossRefGoogle Scholar
- 28.R. M. Schwartz and M. O. Dayhoff, Matrices for detecting distant relationships, in: “Atlas of Protein Sequence and Structure,” Vol. 5, Suppl. 3, M. O. Dayhoff, ed., National Biomedical Research Foundation, Washington (1979).Google Scholar
- 38.M. O. Dayhoff and B. C. Orcutt, Methods for identifying proteins by using partial sequences, Proc. Nat. Acad. Sci. USA 76: 2170Google Scholar