Advertisement

Effects of culture and cryopreservation on human oocyte and embryo ultrastructure and function

  • A. Henry Sathananthan
  • Alan Trounson
Part of the Electron Microscopy in Biology and Medicine book series (EMBM, volume 5)

Abstract

The technique of in vitro fertilization (IVF) involves the removal of the oocyte from the ovary under negative pressure and the exposure of the oocyte to a series of manipulations and solutions that are very different to those experienced in vivo.

Keywords

Human Embryo Zona Pellucida Cumulus Cell Human Oocyte Cortical Granule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Rogers PAW, Milne BJ, Trounson AO: A model to show human uterine receptivity and embryo viability following ovarian stimulation for in vitro fertilization. J Vitro Fert Embryo Transfer 3: 93–98, 1986.CrossRefGoogle Scholar
  2. 2.
    Asch RH, Balmaceda JP, Ellsworth LR, Wong PC: Preliminary experiences with gamete intrafallopian transfer (GIFT). Fertil Steril 45: 366–371, 1986.PubMedGoogle Scholar
  3. 3.
    Sathananthan AH, Trounson, AO, Wood C: Atlas of fine structure of human sperm penetration, eggs and embryos cultured into vitro. Philadelphia,: Praeger Scientific, 1986.Google Scholar
  4. 4.
    Trounson AO, Mohr LR, Wood C, Leeton JF: Effect of delayed insemination on in-vitro fertilization, culture and transfer of human embryos. J Reprod Fert 64: 285–294, 1982.CrossRefGoogle Scholar
  5. 5.
    Sathananthan AH, Trounson AO: Ultrastructural observations on cortical granules in human follicular oocytes cultured in vitro. Gamete Res 5: 191–198, 1982.CrossRefGoogle Scholar
  6. 6.
    Trounson AO, Sathananthan AH: The application of electron microscopy in the evaluation of 2–4 cell human embryos cultured in vitro for embryo transfer. J Vitro Fert Embryo Transfer 1: 153–165, 1984.CrossRefGoogle Scholar
  7. 7.
    Lippes J, Enders RG, Pragay DA, Bartholomew WR: The collection and analysis of human fallopian tubal fluid. Contraception 5: 85–87, 1972.PubMedCrossRefGoogle Scholar
  8. 8.
    Trounson A: In vitro fertilization and embryo preservation. In: In Vitro Fertilization and Embryo Transfer. A Trounson C Wood (eds), Edinburgh: Churchill Livingstone, p 111–130, 1984.Google Scholar
  9. 9.
    Menezo Y, Testart J, Perrone D: Serum is not necces-sary in human in vitro fertilization, early embryo culture and transfer. Fertil Steril 42: 750–755, 1984.PubMedGoogle Scholar
  10. 10.
    Quinn P, Kerin JF, Warnes GM: Improved pregnancy rate in human in vitro fertilization with the use of medium based on the composition of human tubal fluid. Fertil Steril 44: 493–498, 1985.PubMedGoogle Scholar
  11. 11.
    Gianaroli L, Seracchioli R, Ferraretti AP, Trounson A, Flamigni C, Bovicelli L: The successful use of human amniotic fluid for mouse embryo culture and human in vitro fertilization, embryo culture and transfer. Fertil Steril 46: 907–911, 1986.PubMedGoogle Scholar
  12. 12.
    Mohr LR, Trounson AO: The use of fluorescein dia-cetate to assess embryo viability in the mouse. J Reprod Fert 58: 189–196, 1980.CrossRefGoogle Scholar
  13. 13.
    Trounson A: Preservation of human eggs and embryos. Fertil Steril 46: 1–12, 1986.PubMedGoogle Scholar
  14. 14.
    Mohr LR, Trounson AO, Leeton JF, Wood C: Evaluation of normal and abnormal human embryo development during procedures in vitro. In: Fertilization of the Human Egg In Vitro. HM Beier, HR Lindner (eds), Berlin: Springer Verlag, p 211–221, 1983.Google Scholar
  15. 15.
    Trounson A, Mohr L: Human pregnancy following cryo-preservation, thawing and transfer of an eight-cell embryo. Nature 305: 707–709, 1983.PubMedCrossRefGoogle Scholar
  16. 16.
    Mohr LR, Trounson A, Freemann L: Deep-freezing and transfer of human embryos. J Vitro Fert Embryo Transfer 2: 1–10, 1985.CrossRefGoogle Scholar
  17. 17.
    Mohr LR, Trounson AO: Structural changes associated with freezing of bovine embryos. Biol Reprod 25: 1009–1025, 1981.PubMedCrossRefGoogle Scholar
  18. 18.
    Whittingham DG: Fertilization in vitro and development to term of unfertilized mouse oocytes previously stored at -196°C. J Reprod Fertil 49: 89–94, 1977.PubMedCrossRefGoogle Scholar
  19. 19.
    Glenister PH, Wood MJ, Kirby C, Whittingham DG: The incidence of chromosome anomalies in first-cleavage mouse embryos obtained from frozen-thawed oocytes fertilized in vitro. Gamete Res 16: 205–216, 1987.PubMedCrossRefGoogle Scholar
  20. 20.
    Chen C: Pregnancy after human oocyte cryopreserva-tion. Lancet 1: 884–886, 1986.PubMedCrossRefGoogle Scholar
  21. 21.
    Rall WF, Fahy GM: Ice-free cryopreservation of mouse embryos at -196°C by vitrification. Nature: 573–575, 1985.Google Scholar
  22. 22.
    Lasalle B, Testart J, Renard JP: Human embryo features that influence the success of cryopreservation with the use of 1,2 propanediol. Fertil steril 44: 645–651, 1985.Google Scholar
  23. 23.
    Peura A, Trounson A, Freemann L: Ultra-rapid embryo freezing (Abstr). Presented at the Fourth World Congress on In Vitro Fertilization, Melbourne, Australia, 1985, p 244.Google Scholar
  24. 24.
    Schneider U: Cryobiological principles of embryo freezing. J Vitro Fertil Embryo Transfer 3: 3–9, 1986.CrossRefGoogle Scholar
  25. 25.
    Sathananthan AH, Ng SC, Edirisinghe R, Ratnam SS: Sperm-oocyte interaction in the human during Polyspermic fertilisation in vitro. Gamete Res 15: 317–326, 1986.CrossRefGoogle Scholar
  26. 26.
    Sathananthan AH: Ultrastructural morphology of fertilization and early cleavage in the human. In: In Vitro Fertilization and Embryo Transfer. A Trounson, C Wood (eds), London: Churchill Livingstone, p 131–158, 1984.Google Scholar
  27. 27.
    Cohen J, Simons RF, Fehilly CB, Edwards RG: Factors affecting survival and implantation of cryopreserved human embryos. J Vitro Fertil Embryo Transfer 3: 46–52, 1986.CrossRefGoogle Scholar
  28. 28.
    Freemann L, Trounson, A, Kirby C: Cryopreservation of human embryos: progress on the clinical use of the technique in human IVF. J Vitro Fert Embryo Transfer 3: 53–61, 1986.CrossRefGoogle Scholar
  29. 29.
    Sathananthan AH, Chen C: Sperm-oocyte membrane fusion in the human during monospermic fertilization. Gamete Res 15: 177–186, 1986.CrossRefGoogle Scholar
  30. 30.
    Cohen J, Simons RF, Edwards RG, Fehilly CB, Fishel SB: Pregnancies following the frozen storage of expanding human blastocysts. J Vitro Fertil Embryo Transfer 2: 59–64, 1985.CrossRefGoogle Scholar
  31. 31.
    Fawcett DW: Bloom and Fawcett: A Textbook of Histology. Philadelphia: Saunders, 1986.Google Scholar
  32. 32.
    Van Blerkom J, Henry GP, Porreco RP: Preimplanta-tion human embryonic development from polynuclear eggs after in vitro fertilization. Fertil Steril 41: 686–696, 1984.PubMedGoogle Scholar
  33. 33.
    Sathananthan AH, Trounson AO, Freemann L: Morphology and fertilizability of frozen human oocytes. Gamete Res 16: 343–354, 1987.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers, Boston 1989

Authors and Affiliations

  • A. Henry Sathananthan
    • 1
  • Alan Trounson
  1. 1.Lincoln School of Health SciencesLaTrobe UniversityCarltonAustralia

Personalised recommendations