Neuronal Fibrillar Cytoskeleton and Endomembrane System Organization in Alzheimer’s Disease
- 40 Downloads
We examined the structural organization of cytoskeletal components and membrane systems in neurons of well preserved biopsy material from Alzheimer’s disease (AD). Information was obtained from thin sectioned material using conventional electron microscopy and from thick sections with the high voltage electron microscope. Stereo viewing and computer assisted serial reconstruction techniques were employed to visualize three-dimensional relationships among cytoplasmic components of cortical neurons.
We observed several interesting ultrastructural features in AD neurons. These include associations of paired helical filaments (PHF) with the membranes of the nuclear envelope and with ribosomes, differences between the distribution of the Golgi apparatus in neurons containing paired helical filament bundles as compared to neurons without these filaments, and abnormalities in the microtubules of neuronal processes in the vicinity of neuritic plaques.
KeywordsGolgi Apparatus Nuclear Envelope Neuritic Plaque Stereo Pair Open Arrow
Unable to display preview. Download preview PDF.
- Campbell, M.J., Lewis, D.A. and Morrison, J.H. (1985) The immunohistochemical distribution of somatostatin 28 (SS28) and somatostatin 28 (1–12) (SS28(1–12)) in monkey prefrontal (PFC) and temporal (TC) cortical regions. Soc. Neurosci. Abstr. 11:679.Google Scholar
- Ellisman, M.H. and Lindsey, J.D. (1982) Organization of axoplasm: membranous and fibrillar components possibly involved in fast neuroplasmic transport. In Axoplasmic Transport (ed. D. Weiss), Raven Press, NY, pp. 55–63.Google Scholar
- Ellisman, M.H., Ranganathan, R., Deerinck, T.J., Young, S.J., Terry, R. and Mirra, S. (1986) Fibrillar cytoskeleton and endomembrane system organization in Alzheimer neurons. Soc. Neurosci. Abstr. 12: 268.Google Scholar
- Golgi, C. (1898) Intorno alia struttura delle cellule nervose. Boll. Soc. Med. Pavia 1: 3–16.Google Scholar
- Golgi, C. (1899) Di nuovo sulla struttura delle cellule nervose dei gangli spinali. Arch. Ital. Biol. 31: 273–180.Google Scholar
- Golgi, C. (1900) Intorno alia struttura delle cellule nervose della corteccia cerebrale. Verhand. cL Anat. Gesell. 14: 164–176.Google Scholar
- Lewis, D.A., Campbell, M.J., Huntley, G.W., Benson, D.L., Terry, R.D. and Morrison, J.H. (1986a) Laminar and regional specificity of tangle and plaque distribution in Alzheimer’s disease (AD): A quantitative study of visual and auditory cortices. Soc. Neurosci. Abstr. 12.Google Scholar
- Peters, A., Palay, S.L., and Webster, H. deF. (1976) “The Fine Structure of the Nervous System,” Saunders, Philadelphia.Google Scholar
- Strehler, B.L. (1976) Time, Cells, and Aging, Academic Press, NY.Google Scholar
- Terry, R.D., Peck, A., DeTeresa, R., Schechter, R. and Horoupian, D.A. (1981) Some morphometric aspects of the brain in senile dementia of the Alzheimer type. Neurology 10: 184–192.Google Scholar
- Wisniewski, H.M. and Terry, R.D. (1976) Neuropathology of the aging brain. In Neurobiology of Aging (ed., R.D. Terry and S. Gershon), Raven Press, NY, pp. 265–280.Google Scholar
- Young, S.J., Royer, S., Groves, P.M., and Kinnamon, J.C. (1987) An IBM PC-based system for three-dimensional reconstruction from serial sections, J. Electron. Microsc. Techniq., in press.Google Scholar