Cardiac Sarcolemmal Vesicles: ATP-Dependent Ca Ion Transport and Inhibition of Protein Kinase Reactions by Amrinone

  • Roland Vetter
  • Hannelore Haase
  • Liane Will-Shahab
Part of the Developments in Cardiovascular Medicine book series (DICM, volume 102)


In the cardiac sarcolemma (SL), several Ca2+-transporting systems control cytosolic Ca2+ concentration which is linked to the contraction/relaxation cycle of the heart. 1 Ca2+ ions enter the myocardial cell during each excitation event via dihydropyridine-sensitive, voltage-operated Ca2+ channels.2,3 Both an electrogenic Na+/Ca2+ exchange process 4,5 and an ATP-driven Ca2+ pump participate in the extrusion of Ca2+ from the cell. These Ca2+ transport processes may be modulated by several membrane-associated protein kinases through protein phosphorylation reactions which are controlled by intracellular messengers like cyclic AMP, Ca2+, cyclic GMP and diacylglycerol.7–9 This modulation is a basic mechanism of mediation of the cellular response to a number of inotropic and chronotropic neurotransmitters, hormones and other agents. For example, it is well documented that the positive inotropic response to β-adrenergic agents includes SR Ca2+ pump activation via cyclic AMP and Ca2+/calmodulin-dependent phosphorylation of the membrane proteolipid phospholamban. 10,11 SL membrane contains several protein kinases which are controlled either by cyclic AMP, Ca2+/calmodulin or Ca2+/phospholipid. They catalyse the phosphorylation of different membrane substrates. 7,9 In SL, β-adrenergic stimulation or injection of cyclic AMP-dependent protein kinase into single cardiomyocytes is known to result in enhanced Ca2+ flux through slow Ca2+ channels. 12,13 Na+exchange in cardiac SL vesicles can be regulated by Ca2+/calmodulin-dependent phosphorylation/dephosphorylation reaction. 14


Dependent Protein Kinase Cardiac Sarcoplasmic Reticulum Cardiac Sarcolemma Heart Sarcolemma Cardiac Sarcolemmal Vesicle 


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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Roland Vetter
    • 1
  • Hannelore Haase
    • 1
  • Liane Will-Shahab
    • 1
  1. 1.Central Institute for Cardiovascular Research, Division of Cellular and Molecular CardiologyAcademy of Sciences of the GDRBerlin-BuchGermany

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