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Immunohistochemistry of Lung Cancer: Cell differentiation and Growth Properties

  • Yukio Shimosato
  • Setsuo Hirohashi
  • Takashi Nakajima
  • Masayuki Noguchi
Part of the Cancer Treatment and Research book series (CTAR, volume 45)

Abstract

No conspicuous progress was made in the field of diagnostic or surgical pathology until the discovery of enzyme immunohistochemistry [1,2]. With this method it has become possible to visualize various intra- and extracellular substances that could not be identified by conventional histochemical methods on histology sections. The method itself is not complicated and can be performed routinely by histopathology technicians.

Keywords

Pleomorphic Adenoma Sugar Chain Secretory Component Bronchial Gland Aberrant Glycosylation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Sternberger LA: The unlabelled antibody peroxidase-antiperoxidase (PAP) method. In: Immunohistochemistry. Second edition. New York, John Wiley & Sons, 1979, pp 104–109.Google Scholar
  2. 2.
    Hsu SM, Raine K, Fanger H: Use of avidin-biotin peroxidase complex (ABC) in immuno-peroxidase techniques: A comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580, 1981.PubMedCrossRefGoogle Scholar
  3. 3.
    Osborn M, Weber K: Tumor diagnosis by intermediate filament typing: A novel tool for surgical pathology. Lab Invest 48:372–394, 1983.PubMedGoogle Scholar
  4. 4.
    Yamaguchi K, Abe K, Adachi I, Kimura S, Suzuki M, Shimada A, Kodama T, Kameya T, Shimosato Y: Peptide hormone production in primary lung tumors. In: Havemann K, Sorenson G, Gropp G (eds.): Recent Results in Cancer Research. Volume 99. Heidelberg, Springer-Verlag, 1985, pp 107–116.Google Scholar
  5. 5.
    Carney DN, Marangos PJ, Idhe DC, Bunn PA Jr, Cohen MH, Minna JD, Gazdar AF: Serum neuron-specific enolase: A marker for disease extent and response to therapy of small cell lung cancer. Lancet 1:583–585, 1982.PubMedCrossRefGoogle Scholar
  6. 6.
    Gazdar AF, Zweig MH, Carney DN, van Steirteghen AC, Baylin SB, Minna JD: Levels of creatine kinase and its BB isoenzyme in lung cancer specimens and cultures. Cancer Res 41:2773–2777, 1981.PubMedGoogle Scholar
  7. 7.
    Shimosato Y: Lung cancer: Its histogenesis, differentiation and prognostic factors. Tr Soc Pathol Jpn 72:29–57 (in Japanese), 1983.Google Scholar
  8. 8.
    Singh G, Katyal SL, Torikata C: Carcinoma of type II penumocytes. Immunodiagnosis of a subtype of ‘bronchioloalveolar carcinoma.’ Am J Pathol 102:195–208, 1981.PubMedGoogle Scholar
  9. 9.
    Kuroki Y, Dempo K, Akino T: Immunohistochemical study of human pulmonary surfactant apoproteins with monoclonal antibody. Pathologic application for hyaline membrane disease. Am J Pathol 124:25–33, 1986.PubMedGoogle Scholar
  10. 10.
    Mizutani Y, Nakajima T, Morinaga S, Gotoh M, Shimosato Y, Akino T, Suzuki A: Immunohistochemical localization of pulmonary surfactant apoproteins in various lung tumors, with special reference to lung adenocarcinoma subtypes. Cancer 61:532–537, 1988.PubMedCrossRefGoogle Scholar
  11. 11.
    Hirohashi S, Ino Y, Kodama T, Shimosato Y: Distribution of blood group antigens A, B, H and I(Ma) in mucus-producing adenocarcinoma of human lung. J Natl Cancer Inst 72:1299–1305, 1984.PubMedGoogle Scholar
  12. 12.
    Hirohashi S, Clausen H, Yamada T, Shimosato Y, Hakomori S: Blood group A cross-reacting epitope defined by monoclonal antibodies NCC-Lu-35 and -81 expressed in cancer of blood group O or B individuals. Its identification as Tn antigen. Proc Natl Acad Sci USA 82:7039–7043, 1985.PubMedCrossRefGoogle Scholar
  13. 13.
    Dolbeare F, Gratzner HG, Pallavicini MG, Gray JW: Flowcytometric measurement of total DNA content and incorporated Bromodeoxyuridine. Proc Natl Acad Sci USA 80:5973–5577, 1983.CrossRefGoogle Scholar
  14. 14.
    Tada T, Kodama T, Watanabe S, Sato Y, Shimosato Y: Immunohistochemical cell kinetic study of human lung cancer by using monoclonal antibody to bromodeoxyuridine. Jpn J Clin Oncol 16:129–135, 1986.PubMedGoogle Scholar
  15. 15.
    Veale D, Ashcroft T, Marsh C, Gibson GJ, Harris AL: Epidermal growth factor receptors in non-small cell lung cancer. Br J Cancer 55:513–516, 1987.PubMedCrossRefGoogle Scholar
  16. 16.
    Sato Y, Watanabe S, Kodama T, Goto M, Shimosato Y: Stainability of lung cancer cells with Leu-7 and OKT-9 monoclonal antibodies. Jpn J Clin Oncol 15:537–544, 1985.PubMedGoogle Scholar
  17. 17.
    Cuttitta F, Carney DN, Mulshine J, Moody TW, Fedorko J, Fischler A, Minna JD: Bombesin-like peptide can function as autocrine growth factors in human small-cell lung cancer. Nature 316:823–826, 1985.PubMedCrossRefGoogle Scholar
  18. 18.
    Noguchi M, Hirohashi S, Shimosato Y, Thor A, Schlom J, Tsunokawa Y, Terada M, Sugimura T: Histologic demonstration of antigens reactive with anti-p21 ras monoclonal antibody (RAP-5) in human stomach cancer. J Natl Cancer Inst 77:379–385, 1986.PubMedGoogle Scholar
  19. 19.
    Kanai T, Hirohashi S, Noguchi M, Shimoyama Y, Shimosato Y, Noguchi S, Nishimura S, Abe O: Monoclonal antibody highly sensitive for the detection of ras p21 in immunoblotting analysis. Jpn J Cancer Res (Gann) 78:1314–1318, 1987.Google Scholar
  20. 20.
    Liotta LA, Rao CN, Barsky SH: Tumor invasion and the extracellular matrix. Lab Invest 49:636–649, 1983.PubMedGoogle Scholar
  21. 21.
    Roos E: Cellular adhesion, invasion and metastasis. Biochem Biophys Acta 738:263–284, 1984.PubMedGoogle Scholar
  22. 22.
    Shimosato Y, Nakajima T, Hirohashi S, Morinaga S, Terasaki T, Yamaguchi K, Saijo N, Suemasu K: Biological, pathological and clinical features of small cell lung cancer. Cancer Lett 33:241–258, 1986.PubMedCrossRefGoogle Scholar
  23. 23.
    Watanabe J, Okabe T, Fujisawa M, Takaku F, Hirohashi S, Shimosato Y: Monoclonal antibody that distinguishes small-cell lung cancer from non-small-cell lung cancer. Cancer Res 47:826–829, 1987.PubMedGoogle Scholar
  24. 24.
    Hirohashi S, Kunii T, Hirano T, Shimosato Y: 145 kDa cell membrane antigen on SCLC as a common target for several monoclonal antibodies raised against SCLCs. Presented at the First Workshop on Small Cell Lung Cancer Antigens, London, April 28–29, 1987.Google Scholar
  25. 25.
    Yamaguchi K, Abe K, Adachi I et al: Peptide hormone production in small cell lung carcinomas with particular reference to gastrin-releasing peptide. Jpn J Clin Oncol 16:235–241, 1986.PubMedGoogle Scholar
  26. 26.
    Lloyd RV, Sisson JC, Shapiro B, Verhofstad AAJ: Immunohistochemical localization of epinephrine, norepinephrine, catecholamine-synthesizing enzymes, and chromogranin in neuroendocrine cells and tumors. Am J Pathol 125:45–54, 1986.PubMedGoogle Scholar
  27. 27.
    Nakajima T, Shimosato Y, Morinaga S, Terasaki T, Tsumuraya M, Yamaguchi K, Ichinose K, Nagatsu T, Kato K, Nakazato Y: Immunohistochemical study of small cell lung carcinoma, with special reference to neuroendocrine markers such as aromatic L-amino acid decarboxylase and gastrin releasing peptide. Jpn J Clin Oncol 16:223–233, 1986.PubMedGoogle Scholar
  28. 28.
    Nagatsu T, Ichinose H, Kojima K, Kameya T, Shimase J, Kodama T, Shimosato Y: Aromatic L-amino acid decarboxylase activities in human lung tissues: comparison between normal lung and lung carcinomas. Biochem Med 34:52–59, 1985.PubMedCrossRefGoogle Scholar
  29. 29.
    Nomori H, Shimosato Y, Kodama T, Morinaga S, Nakajima T, Watanabe S: Subtypes of small cell carcinoma of the lung: Morphometric, ultrastructural and immunohistochemical analyses. Human Pathol 17:604–613, 1986.CrossRefGoogle Scholar
  30. 30.
    Yesner R: Classification of lung cancer histology. N Engl J Med 312:652–653, 1985.PubMedGoogle Scholar
  31. 31.
    Matthews MJ, Gazdar AF: Small cell carcinoma of the lung: Its morphology, behavior and nature. In: Shimosato Y, Melamed MR, Nettesheim P (eds.): Morphogenesis of Lung Cancer. Volume 2. Boca Raton, CRC Press, 1982, pp 1–4.Google Scholar
  32. 32.
    Tsumuraya M, Kodama T, Shimosato Y, Koketsu H, Uei Y: Light and electron microscopic analysis of intranuclear inclusion in papillary adenocarcinoma of the lung. Acta Cytol 25:523–532, 1981.PubMedGoogle Scholar
  33. 33.
    Torikata C, Ishiwata K: Intranuclear tubular structures observed in the cells of an alveolar cell carcinoma of the lung. Cancer 40:1194–1201, 1977.PubMedCrossRefGoogle Scholar
  34. 34.
    Mori K, Kodama T, Shimosato Y: Analysis of cytodifferentiation in squamous cell carcinoma of the lung in relation to the site of origin, sex and smoking history. Lung Cancer 26:117–123 (in Japanese), 1986.Google Scholar
  35. 35.
    Shimosato Y, Kodama T: Low grade malignant and benign tumors. In: McDowell EM (ed.): Lung Carcinoma. Edinburgh, London, Melbourne, New York, Churchill Livingstone, 1987, pp 310–329.Google Scholar
  36. 36.
    Upton M, Hirohashi S, Tome Y, Miyazawa N, Suemasu K, Shimosato Y: Expression of vimentin in surgically resected adenocarcinomas and large cell carcinomas of lung. Am J Surg Pathol 10:560–567, 1986.PubMedCrossRefGoogle Scholar
  37. 37.
    Morinaga S, Nakajima T, Shimosato Y: Normal and neoplastic myoepithelial cells in salivary glands. An immunohistochemical study. Human Pathol 18:1218–1226, 1987.CrossRefGoogle Scholar
  38. 38.
    Tsutsumi Y, Nagura H, Watanabe K: Immunohistochemical observations of carcinoem-bryonic antigen (CEA) and CEA-related substances in normal and neoplastic pancreas. Pitfalls and caveats in CEA immunohistochemistry. Am J Clin Pathol 82:535–542, 1984.PubMedGoogle Scholar
  39. 39.
    Morinaga S, Shimosato Y: Microcancer of bronchus and lung: Pathology of microcancer of peripheral lung origin. Byori to Rinsho (Pathology and Clinical Medicine) 5(Suppl):74–80 (in Japanese), 1986.Google Scholar
  40. 40.
    Burger PC, Shibata T, Kleihues P: The use of monoclonal antibody Ki-67 in the identification of proliferating cells: Application to surgical neuropathology. Am J Surg Pathol 10:611–617, 1986.PubMedCrossRefGoogle Scholar
  41. 41.
    Nau MM, Brooks BJ, Battey J, Carney DN, Gazdar AF, Battey JF, Sausville EA, Minna JD: L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature 318:69–73, 1985.PubMedCrossRefGoogle Scholar
  42. 42.
    Rodenhuis S, van de Wetering ML, Mooi WJ, Evers SG, van Zandwijk N, Bos JL: Mutational activation of the k-ras oncogene. A possible pathogenetic factor in adenocarcinoma of the lung. N Engl J Med 317:929–935, 1987.PubMedCrossRefGoogle Scholar
  43. 43.
    Taya Y, Hosogai K, Hirohashi S, Shimosato Y, Tsuchiya R, Tsuchida N, Fushimi M, Sekiya T, Nishimura S: A novel combination of K-ras and myc amplification accompanied by point mutational activation of K-ras in a human lung cancer. EMBO J 3:2943–2946, 1984.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers, Boston 1989

Authors and Affiliations

  • Yukio Shimosato
  • Setsuo Hirohashi
  • Takashi Nakajima
  • Masayuki Noguchi

There are no affiliations available

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