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Light Microscopic Cytology of Cerebrospinal Fluid

  • Claire E. Meena
  • William H. Olson
  • Lung T. Yam

Abstract

Although previously covered in Chapter 4, the following points on gross inspection of cerebrospinal fluid should be reemphasized:
  1. 1.

    Simply looking at a tube of cerebrospinal fluid and calling it clear and colorless is not adequate;

     
  2. 2.

    The cerebrospinal fluid should be immediately spun down after collection and decanted into a separate tube. An identical tube filled with water to the same level should be used for comparison;

     
  3. 3.

    One should look down vertically through the entire length of the tube onto a white background. Daylight, rather than artificial light, should be used if at all possible; and

     
  4. 4.

    Yellowish tints in cerebrospinal fluid are due to many abnormalities, including elevated protein, lysed red blood cells, increased levels of bilirubin, and carotenemia.

     

Keywords

Herpes Zoster Primary Central Nervous System Lymphoma Cryptococcal Meningitis Mycosis Fungoides Immunocytochemical Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Astaldi A, Pasino M, Rosanda C, Massimo L (1976). T and B cells in acute lymphoblastic leukemias. N Engl J Med 294:550551.Google Scholar
  2. 2.
    Barrett D, King E (1976). Comparison of cellular recovery rates and morphologic detail obtained using membrane filter and cytocentrifuge techniques. Acta Cytol 20:174–180.PubMedGoogle Scholar
  3. 3.
    Benjamin DR, Ray CG (1975). Use of immunoperoxidase on brain tissue for the rapid diagnosis of herpes encephalitis. Am J Clin Pathol 64:472–476.PubMedGoogle Scholar
  4. 4.
    Bodensteiner DC, Skikene B (1982). Central nervous system involvement in mycosis fun-goides. Diagnosis, treatment and literature review. Cancer 50:1181–1184.PubMedCrossRefGoogle Scholar
  5. 5.
    Brinkman CJ, Nillesen WA, Hommes OR (1983). T-cell subpopulations in blood and cerebrospinal fluid of multiple sclerosis patients: effect of cyclophosphamide. Clin Immunol Immunopathol 29:341–348.PubMedCrossRefGoogle Scholar
  6. 6.
    Burechialo F, Cunningham T (1974). Counting cells in cerebrospinal fluid collected directly on membrane filters. J Clin Pathol 27:101–105.CrossRefGoogle Scholar
  7. 7.
    Casper JT, Lauer SJ, Kirchner PA, Gottschall JL, Camita BM (1983). Evaluation of cerebrospinal fluid mononuclear cells obtained from children with acute lymphocytic leukemia: advantages of combining cytomorphology and terminal deoxynucleotidyl transferase. Am J Clin Pathol 80:666–670.PubMedGoogle Scholar
  8. 8.
    Chow G, Schmidley W (1984). Lysis of erythrocytes and leukocytes in traumatic lumbar puncture. Arch Neurol 41:1084–1085.PubMedGoogle Scholar
  9. 9.
    Clark G (ed.) (1973). Staining Procedures. Third edition. Baltimore: Williams and Wilkins, pp. 122–123.Google Scholar
  10. 10.
    Coakham HB, Garson JA, Brownell B, Allan PM, Harper EI, Lane B, Kemshead JT (1984). Use of monoclonal antibody panel to identify malignant cells in cerebrospinal fluid. Lancet 1:1095–108.PubMedCrossRefGoogle Scholar
  11. 11.
    Collins VP (1984). Monoclonal antibodies to glial fibrillary acidic protein in the cytologic diagnosis of brain tumors. Acta Cytol 28:401–406.PubMedGoogle Scholar
  12. 12.
    Cordell JL, Falini B, Erbner WN, Ghasl AK, Abdulaziz Z, McDonald S, Pulford KAF, Stein H, Mason DY (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229.PubMedCrossRefGoogle Scholar
  13. 13.
    Dayan AD, Stokes MI (1973). Rapid diagnosis of encephalitis by immunofluorescent examination of cerebrospinal fluid cells. Lancet 1:177–179.PubMedCrossRefGoogle Scholar
  14. 14.
    Ducos R, Donaso J, Weikhardt U, Vietti T (1979). Sedimentation versus cytocentrifugation in the cytologic study of craniospinal fluid. Cancer 43:1479–1482.PubMedCrossRefGoogle Scholar
  15. 15.
    Dyken PR, Shirley BS, Trefz J, El Gammal T (1980). Comparison of cytocentrifugation and sedimentation techniques for CSF cytomorphology. Acta Cytol 24:167–170.PubMedGoogle Scholar
  16. 16.
    Gondos B (1986). Millipore filter vs. cytocentrifuge for evaluation of cerebrospinal fluid. Arch Pathol Lab Med 110:687–688.PubMedGoogle Scholar
  17. 17.
    Goodson JN, Strauss GM (1979). Diagnosis of lymphomatous leptomeningitis by cerebrospinal fluid lymphocyte cell surface markers. Am J Med 66:1057–1059.PubMedCrossRefGoogle Scholar
  18. 18.
    Hancock WW, Medlby S (1983). Monoclonal antibodies to identify tumor cells in CSF. Lancet 2:739–740.PubMedCrossRefGoogle Scholar
  19. 19.
    Hansen H, Bender R, Shelton B (1974). The cyto-centrifuge and cerebrospinal fluid cytology. Acta Cytol 18:259–262.PubMedGoogle Scholar
  20. 20.
    Hartog Jager WA Den (1980). Color Atlas of CSF. Philadelphia: J.B. Lippincott Co.Google Scholar
  21. 21.
    Homans AC, Forman EN, Barker BE (1985). Use of monoclonal antibodies to identify cerebrospinal fluid lymphoblasts in children with acute lymphoblastic leukemia. Blood 66:1321–1325.PubMedGoogle Scholar
  22. 22.
    Jones GR, Mason WH, Fishman LS, Declerck YA (1985). Primary central nervous system lymphoma without intracranial mass in a child. Diagnosis by documentation of mono-clonality. Cancer 56:2804–2808.PubMedCrossRefGoogle Scholar
  23. 23.
    Juozevicus JL, Rynes RI (1986). Increased helper/suppressor T-lymphocyte ratio in the cerebrospinal fluid of a patient with neurosarcoidosis. Ann Intern Med 104:807–808.Google Scholar
  24. 24.
    Kobayashi TK, Yamaki T, Yoshino E, Higuchi T, Kamachi M (1984). Immunocytochemical demonstration of carcinoembryonic antigen in cerebrospinal fluid with carcinomatous meningitis from rectal cancer. Acta Cytol 28:430–434.PubMedGoogle Scholar
  25. 25.
    Levinson AI, Lisak RP, Zweiman B (1976). Immunologic characterization of cerebrospinal fluid lymphocytes: preliminary report. Neurology 26:693–695.PubMedGoogle Scholar
  26. 26.
    Li CY, Lazcano-Villareal O, Pierre RV, Yam LT (1987). Immunocytochemical identification of cells in serous effusion: technical considerations. Am J Clin Pathol 88:696–706.PubMedGoogle Scholar
  27. 27.
    Li CY, Witzig TE, Phyliky RL, Ziesmer S, Yam LT (1986). Diagnosis of B cell non-Hodgkin’s lymphoma of the central nervous system by immunocytochemical analysis of cerebrospinal fluid lymphocytes. Cancer 57:737–744.PubMedCrossRefGoogle Scholar
  28. 28.
    Li CY, Ziesmer SC, Lazacano-Villareal O (1987). Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method. J Histochem Cytochem 35:1459–1466.CrossRefGoogle Scholar
  29. 29.
    Manconi PE, Zaccheo D, Bugiani O, Fadda MF, Cadoni A, Marrosu MG, Cianchetti C, Grifoni V (1978). Surface markers on lymphocytes from human cerebrospinal fluid. Predominance of T lymphocytes bearing receptors for the Fc segment of IgG. Eur Neurol 17:87–91.PubMedCrossRefGoogle Scholar
  30. 30.
    Manconi PE, Zaccho D, Bugiani O, Fadda MF, Grifoni V, Montovani G, Del Giacco GS, Tognella S (1976). T and B lymphocytes in normal cerebrospinal fluid. N Engl J Med 294:49.PubMedGoogle Scholar
  31. 31.
    Mann RB, Jaffe ES, Braylan RC, Eggleston JC, Ranson L, Kaizer H, Bernard CS (1975). Immunologic and morphologic studies of T cell lymphoma. Am J Med 58:307–313.PubMedCrossRefGoogle Scholar
  32. 32.
    Marsh WL Jr, Stevenson DR, Long HJ III (1983). Primary leptomeningeal presentation of T cell lymphoma. Report of a patient and review of the literature. Cancer 51:1125–1131.PubMedCrossRefGoogle Scholar
  33. 33.
    Martin SE, Zhang HZ, Magyarosy E, Jaffe ES, Hsu SM, Chu EW (1984). Immunologic methods in cytology: definitive diagnosis of non-Hodgkin’s lymphomas using immunologic markers for T and B cells. Am J Clin Pathol 82:666–673.PubMedGoogle Scholar
  34. 34.
    Moser RP, Robinson JA, Prostko ER (1976). Lymphocyte subpopulations in human cerebrospinal fluid. Neurology 26:726–728.PubMedGoogle Scholar
  35. 35.
    Nakamura S, Takase S, Itahara K, Ogata M, Shigeta S (1981). Cytological and immunological examination of cerebrospinal fluid in nine patients with Ramsey-Hunt’s syndrome. Tohoku J Exp Med 133:121–128.PubMedCrossRefGoogle Scholar
  36. 36.
    Oehmichen A (1976). Cerebrospinal Fluid Cytology. Philadelphia: W.B. Saunders Co.Google Scholar
  37. 37.
    Oehmichen M, Huber H (1978). Supplementary cytodiagnostic analysis of mononuclear cells of the cerebrospinal fluid using cytological markers. J Neurol 218:187–196.PubMedCrossRefGoogle Scholar
  38. 38.
    Reynaud A, King E (1967). A new filter for diagnostic cytology. Acta Cytol 11:289–294.PubMedGoogle Scholar
  39. 39.
    Rich J (1972). A membrane filter technique for cerebrospinal fluid cytology. J Neurosurg 36:661–666.PubMedCrossRefGoogle Scholar
  40. 40.
    Richert JR, Rose JW, Xu XH, Greenstein JI, McFarland HF, McFarlin DE (1984). Characterization of cerebrospinal fluid lymphocytes in multiple sclerosis. Ann NY Acad Sci 436: 151–162.PubMedCrossRefGoogle Scholar
  41. 41.
    Rosenthal DL (1984). Cytology of the Central Nervous System. Volume 8. In: Weid GL (ed.): Monographs clinical cytology. New York: Karger, pp. 1–12, 171–178.Google Scholar
  42. 42.
    Sandberg-Wollheim M, Turesson I (1975). Lymphocyte subpopulations in the cerebrospinal fluid and peripheral blood in patients with multiple sclerosis. Scand J Immunol 4:831–836.PubMedGoogle Scholar
  43. 43.
    Schädlich HJ, Nekie M, Felgenhauer K (1980). The detection of activated cerebrospinal fluid B lymphocytes by the peroxidase conjugated antibodies. J Neurol 224:77–87.PubMedCrossRefGoogle Scholar
  44. 44.
    Spiers ASD, Halpern R, Ross SC, Neiman RS, Harawi S, Zipoli TE (1980). Meningeal myelomatosis. Arch Intern Med 140:256–259.PubMedCrossRefGoogle Scholar
  45. 45.
    Stark E, Wurster U (1986). Preparation procedure for cerebrospinal fluid that yields cytologic samples suitable for all types of staining, including immunologic and enzymatic methods. Acta Cytol 31:374–376.Google Scholar
  46. 46.
    Stokes H, O’Hara C, Buchanan R, Olson W (1975). An improved method for examination of cerebrospinal fluid cells. Neurology 25:901–906.PubMedGoogle Scholar
  47. 47.
    Sun T, Li CY, Yam LT (1985). Atlas of Cytochemistry and Immunochemistry of Hematologic Neoplasms. Chicago: American Society of Clinical Pathologists Press.Google Scholar
  48. 48.
    Kjeldsberg CR, Krieg AF (1984). Cerebrospinal fluid and other body fluids. In: Henry JB (ed.): Todd, Sanford, Davidson. Clinical Diagnosis and Management by Laboratory Methods. Seventeenth edition. Philadelphia: W.B. Saunders, pp. 462–464.Google Scholar
  49. 49.
    Traugott U (1978). T and B lymphocytes in the cerebrospinal fluid of various neurological diseases. J Neurol 219:185–197.PubMedCrossRefGoogle Scholar
  50. 50.
    Tutarima JA, Hische EAH, Vander Helm HJ (1979). An improved method for the concentration of cerebrospinal fluid cells by suction tip and sedimentation chamber. J. Neurol Sci 44:61–67.CrossRefGoogle Scholar
  51. 51.
    Veerman A, Huismans L, Zantwijk I (1985). Storage of cerebrospinal fluid samples at room temperature. Acta Cytol 29:188–189.PubMedGoogle Scholar
  52. 52.
    Whitmore E, Hochberg F, Wolfson K, Royalty J, Taft P (1982). Quantitative cytocentrifuga-tion in the evaluation of cerebrospinal fluid. Acta Cytol 26:847–850.PubMedGoogle Scholar
  53. 53.
    Witt DH, Zalusky, R, Castella A, Mercer WD (1986). Light chain myeloma with meningeal and pleural involvement. Am J Med 80:1213–1216.PubMedCrossRefGoogle Scholar
  54. 54.
    Wolf P, Russell B, Jacobs P (1974). Immunofluorescence as a diagnostic aid in cryptococcal meningitis. Human Path 5:758–760.CrossRefGoogle Scholar
  55. 55.
    Yam LT, English MC, Janekila AJ, Ziesmer SC, Li CY (1987). Immunochemistry of cerebrospinal fluid. Acta Cytol 31:825–833.PubMedGoogle Scholar
  56. 56.
    Young RC, Howser DM, Anderson T, Fisher RI, Jaffe E, Devita VT (1979). Central nervous system complications of non-Hodgkin’s lymphomas. Am J Med 66:435–443.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Claire E. Meena
  • William H. Olson
  • Lung T. Yam

There are no affiliations available

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