Leishmaniasis pp 589-596 | Cite as

A Simple, Highly Repetitive Sequence in the Leishmania Genome

  • John Ellis
  • Julian Crampton
Part of the NATO ASI Series book series (NSSA, volume 171)

Abstract

Leishmania are simple unicellular protozoa which are tramsitted by the bite of an infected sandfly, producing a spectrum of clinical diseases in manl. Since their discovery in 1903, the organisation of the genome of this clinically important protozoan has received little attention. We have used DNA hybridisation studies to identify many of the repetitive DNA sequences in the Leishamania genome. Here we report the characterisation of the most highly repetitive DNA sequence that we have been able to isolate. Differences in copy number and organisation of this sequence within the Leishmania genome may well contribute to the chromosome size variations previously reported by other investigators.

Keywords

Manifold EDTA Agarose Bromide Electrophoresis 

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • John Ellis
    • 1
  • Julian Crampton
    • 1
  1. 1.Wolfson Unit of Molecular GeneticsLiverpool School of Tropical MedicineLiverpoolUK

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