Leishmaniasis pp 503-513 | Cite as

Assessment of the Use in the Diagnosis of Leishmaniasis of Biotinylated Kinetoplast Derived DNA Sequences

  • Douglas C. Barker
Part of the NATO ASI Series book series (NSSA, volume 171)


Any of the 15 subspecies of Leishmania at present identified as infective to man can cause the single cutaneous lesion which is the commonest clinical sign of the disease. The wide spectrum of symptoms which may result from the progression of the disease can, at least partially, be related to the subspecies of Leishmania. However the well known problems [1] of genuine or apparent self healing, metastisizing complications of cutaneous manifestations of visceral or secondary infections and immunopathology make clinical diagnosis difficult. The epidemiologists have the time consuming tasks of dissection of sandflies and patient culturing of organisms from the infected tissue of reservoir hosts to incriminate vectors and hosts in transmission. Both the clinical diagnosis and the epidemiologists’ problems could be alleviated by a rapid, cheap and accurate method of identification.


Steam Agarose Bromide Electrophoresis Radioactive Isotope 


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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Douglas C. Barker
    • 1
  1. 1.MRC Outstation of NIMR, Molteno Laboratory, Department of PathologyUniversity of CambridgeCambridgeUK

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