Assessment of the Use in the Diagnosis of Leishmaniasis of Biotinylated Kinetoplast Derived DNA Sequences
Any of the 15 subspecies of Leishmania at present identified as infective to man can cause the single cutaneous lesion which is the commonest clinical sign of the disease. The wide spectrum of symptoms which may result from the progression of the disease can, at least partially, be related to the subspecies of Leishmania. However the well known problems  of genuine or apparent self healing, metastisizing complications of cutaneous manifestations of visceral or secondary infections and immunopathology make clinical diagnosis difficult. The epidemiologists have the time consuming tasks of dissection of sandflies and patient culturing of organisms from the infected tissue of reservoir hosts to incriminate vectors and hosts in transmission. Both the clinical diagnosis and the epidemiologists’ problems could be alleviated by a rapid, cheap and accurate method of identification.
KeywordsSteam Agarose Bromide Electrophoresis Radioactive Isotope
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