Leishmaniasis pp 503-513 | Cite as

Assessment of the Use in the Diagnosis of Leishmaniasis of Biotinylated Kinetoplast Derived DNA Sequences

  • Douglas C. Barker
Part of the NATO ASI Series book series (NSSA, volume 171)

Abstract

Any of the 15 subspecies of Leishmania at present identified as infective to man can cause the single cutaneous lesion which is the commonest clinical sign of the disease. The wide spectrum of symptoms which may result from the progression of the disease can, at least partially, be related to the subspecies of Leishmania. However the well known problems [1] of genuine or apparent self healing, metastisizing complications of cutaneous manifestations of visceral or secondary infections and immunopathology make clinical diagnosis difficult. The epidemiologists have the time consuming tasks of dissection of sandflies and patient culturing of organisms from the infected tissue of reservoir hosts to incriminate vectors and hosts in transmission. Both the clinical diagnosis and the epidemiologists’ problems could be alleviated by a rapid, cheap and accurate method of identification.

Keywords

Steam Agarose Bromide Electrophoresis Radioactive Isotope 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Peters, W. (1986) “Leishmaniases in Biology and Medicine” Peters, W. and Killick-Kendrick, R., eds, Academic Press, LondonGoogle Scholar
  2. 2.
    Chance, M.L. and Walton, B.C. (1982) Biochemical Characterization of Leishmania WHO, Geneva. 263–276Google Scholar
  3. 3.
    Arnot, D.E. and Barker, D.CEE. (1981) Biochemical identification ofcutaneous leishmania by analysis of kinetoplast DNA II. Sequence homologies in leishmania kDNA, Mol. Biochem. Parasitol. 3:47–56CrossRefPubMedGoogle Scholar
  4. 4.
    Wirth, D.F. and McMahon-Pratt, D. (1982) Rapid identification of Leishmania species by specific hybridisation of kinetoplast DNA in cutaneous lesions. Proc. Nat. Acad. Sci. USA 79:6999–7003CrossRefPubMedGoogle Scholar
  5. 5.
    Barker, D.C. and Butcher, J. (1983) The use of DNA probes in the identification of leishmaniasis: discrimination between isolates of the Leishmania mexicana and L. braziliensis complexes. Trans. R. Soc. Trop. Med. Hyg. 77:285–297CrossRefPubMedGoogle Scholar
  6. 6.
    Spithill, T.W. and Grumont, R.J. (1984) Identification of species, strains and clones of Leishmania by characterization of kinetoplast DNA mini-circles. Mol. Biochem. Parasitol. 12:217–236CrossRefPubMedGoogle Scholar
  7. 7.
    Lopes, U.G. and Wirth, D.F. (1986) Identification of visceral Leishmania species with cloned sequences of kinetoplast DNA. Mol. Biochem. Parasitol. 20:77–84CrossRefPubMedGoogle Scholar
  8. 8.
    Jackson, P.R., Lawrie, J.M., Stiteler, J.M., Hawkins, D.N., Wohlhieter, J.A. and Rowton, E.D. (1986) Detection and characterization of Leishmania species and strains from mammals and vectors by hybridisation and restriction endonuclease digestion of kinetoplast DNA. Vet. Parasitol. 20:195–215CrossRefPubMedGoogle Scholar
  9. 9.
    Barker, D.C., Gibson, L.J., Kennedy, W.P.K., Nasser, A.A.A. and Williams, R.H. (1986) The potential of using recombinant DNA species-specific probes for the identification of tropical Leishmania. Parasitology 91:S139–S174CrossRefGoogle Scholar
  10. 10.
    Wirth, D.F. and Rogers, W.O. (1985) “Rapid identification of infectious agents” Kingsburg, D.T. and Falbow, S., eds 127–137, Academic Press, New YorkGoogle Scholar
  11. 11.
    Wirth, D.F., Rogers, W.O., Barker, R., Dourado, H., Suesebang, L. and Albuquerque, B. (1986) Leishmaniasis and Malaria: New tools for epidemiologic analysis. Science 234:975–979CrossRefPubMedGoogle Scholar
  12. 12.
    Barker, D.C. (1987) DNA diagnosis of human leishmaniasis. Parasitol. Today. 3:177–184CrossRefPubMedGoogle Scholar
  13. 13.
    Ntambi, J.M. and Englund, P.T. (1985) J. Bio. Chem. 260:5574–5579Google Scholar
  14. 14.
    Kennedy, W.P.K. (1984) Novel identification of differences in the kinetoplast DNA of Leishmania isolated by recombinant DNA techniques and’in situf hybridisation. Mol. Biochem. Parasitol. 12:313–325CrossRefPubMedGoogle Scholar
  15. 15.
    Lawrie, J.M., Jackson, P.R., Stiteler, J.M. and Hockmeyer, W.T. (1985) Identification of pathogenic Leishmania promastigotes by DNA:DNA hybridization with kinetoplast DNA cloned to E. coli Plasmids. Am. J. Trop. Med. Hyg. 34:257–265PubMedGoogle Scholar
  16. 16.
    Rogers, W.O. and Wirth, D.F (1987) Kinetoplast DNA minicircles: Regions of extensive sequence divergence. Proc. Natl. Acad. Sci. USA 84:565–569CrossRefPubMedGoogle Scholar
  17. 17.
    Barker, D.C. (1986) in: “Characterisation of Leishmania sp. by DNA hybridization probes” pp 57, WHO, GenevaGoogle Scholar
  18. 18.
    van Eys, G.J.J.M., Schoone, G.J., Lighart, G.S., Laarman, J.J. and Terpstra, W.J. (1987) Detection of Leishmania parasites by DNA in situ hybridization with non-radioactive probes. Parasitol. Res. 73:199–202CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Douglas C. Barker
    • 1
  1. 1.MRC Outstation of NIMR, Molteno Laboratory, Department of PathologyUniversity of CambridgeCambridgeUK

Personalised recommendations