Advertisement

Leishmaniasis pp 977-983 | Cite as

Transcript Alteration in Leishmania

  • K. Stuart
  • J. E. Feagin
Part of the NATO ASI Series book series (NSSA, volume 171)

Abstract

Recent studies of mitochondrial transcripts, initially in African trypanosomes, have revealed that nucleotides (uridines) not encoded in the genes are added to their transcripts. This transcript alteration is developmentally regulated for some genes, such as the apocytochrome b and cytochrome oxidase II genes, but not others such as MURF2 (Feagin et al., 1987 and 1988; Feagin and Stuart, 1988). In addition, this uridine addition does not occur for all genes. These studies indicate the existence of a genetic regulatory process in Kinetoplastidae unlike any previously described. It has been suggested that the transcript alteration creates initiation codons, corrects frameshifts, and alters the 3′ untranslated regions of transcripts (Benne et al., 1986; Feagin et al., 1987; Feagin and Stuart, 1988). These processes could thus affect the transcript translatability, the nature of the protein product or the stability of the transcripts. In African trypanosomes, where developmental regulation of the production of the mitochondrial respiratory System is apparent (Vickerman, 1985), the transcript alteration process probably plays a role in this regulation. However, little is known about possible developmental regulation of the production of the mitochondrial respiratory System in Leishmania, including those that infect humans (see Blum et al., 1987).

Keywords

Developmental Regulation Leishmania Species Trypanosoma Brucei Mitochondrial Transcript African Trypanosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Benne, R., Van den Burg, J., Brakenhoff, J.B.J., Sloof, P., Van Boom, J.H., Tromp, M.C., 1986, Major transcript of the frameshifted coxll gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA, Cell, 46: 819.PubMedCrossRefGoogle Scholar
  2. Blum, J.J., Davis, D.G., Darling, T.N., and London R.E., 1987, Interrrelationships between glucose and alanine catabolism, ammonia production, and the D-lactate pathway in Leishmania braziliensis. In Leishmaniasis: The first centenary (1885–1985) new strategies for control, Plenum Press, London.Google Scholar
  3. Feagin, J.E., Jasmer, D.P., and Stuart, K., 1987, Developmentally regulated addition of nucleotides within apocytochrome b transcripts in Trypanosoma brucei. Cell, 49: 337.PubMedCrossRefGoogle Scholar
  4. Feagin, J.E., Shaw, J.M., Simpson, L. and Stuart, K. 1988, Creation of AUG initiation codons by addition of uridines within cytochrome b transcripts of kinetoplastids, Proc. Natl. Acad. Sci. USA (in press).Google Scholar
  5. Feagin, J.E. and Stuart, K., 1988, Developmental aspects of uridine addition within mitochondrial transcripts of Trypanosoma brucei. Mol. Cell. Biol. (submitted).Google Scholar
  6. Parsons, M., Nelson, R.G., Watkins, K.P., and Agabian, N., 1984, Trypanosome mRNAs share a common 5′ spliced leader sequence, Cell 38: 309.PubMedCrossRefGoogle Scholar
  7. Powell, L.M., Wallis, S.C., Pease, R.J., Edwards, Y. H., Knott, T.J., and Scott, J., 1987, A novel form of tissue-specifiec RNA processing produces apolipoprotein-B48 in intestine, Cell, 50: 831.PubMedCrossRefGoogle Scholar
  8. Vickerman, K., 1985, Developmental cycles and biology of pathogenic trypanosomes, Brit. Med. Bull., 41:105.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • K. Stuart
    • 1
  • J. E. Feagin
    • 1
  1. 1.Seattle Biomedical Research InstituteSeattleUSA

Personalised recommendations