Methods and Labels in Immunoassay

  • Roger Ekins

Abstract

Immunoassay methods relying on radioisotopic labels have played a major role in medicine and other biologically-related fields (agriculture, veterinary science, the food industry etc) during the past two decades. Their importance has derived from their exploitation both of the “structural specificity” characterizing antibody-antigen reactions and the “detectability” of isotopically-labeled reagents, the latter permitting observation of the binding reactions between exceedingly small concentrations of the key reactants involved. The combination of these features has endowed these methods with unique specificity and sensitivity characteristics, and accounts for their ubiquitous use throughout modern medicine and biology. However, in the past few years, interest has increasingly focussed on so-called “alternative”, non-radioisotopic, methods — techniques which are based on essentially the same analytical principles but which differ in the markers used to label the particular reactant (antibody or analyte) whose distribution following the basic analytical reaction constitutes the assay response.

Keywords

Titanium Albumin Europium Influenza Bilirubin 

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References

  1. Aizawa, M., Kato, S., Suzuki, S., 1977, Immunoresponsive membrane I. Membrane potential change associated with an immunochemical reaction between membrane-bound antigen and free antibody, J.Membr.Sci., 2:125.CrossRefGoogle Scholar
  2. Collins, S., Janata, J., 1982, A critical evaluation of the mechanism of potential response of antigen polymer membranes to the corresponding antiserum, Anal.Chim.Acta., 136:93.CrossRefGoogle Scholar
  3. Dakubu, S., Ekins, R., Jackson, T., Marshall, N. J., 1984, High sensitivity, pulsed light time-resolved fluoroimmunoassay, in: “Practical Immunoassay. The State of the Art,” W.R. Butt, ed., Marcel Dekker, Inc., p. 71. New York.Google Scholar
  4. Ekins, R. P., 1983, Measurement of analyte concentration, British Patent no. 8224600.Google Scholar
  5. Ekins, R., 1985, Current concepts and future developments, in: “Alternative Immunoassays,” W.P. Collins, ed., John Wiley & Sons Ltd., p.219. Chichester.Google Scholar
  6. Ekins, R. P., Filetti, S., Kurtz, A. B., Dwyer, K., 1980, A simple general method for the assay of free hormones (and drugs); its application to the measurement of serum free thyroxine levels and the bearing of assay results on the ‘free thyroxine’ concept, J.Endocrinol., 85:29.Google Scholar
  7. Ezzell, C., 1987, Hybritech wins court injunction over sandwich assays, ed., Nature, 327:5.PubMedGoogle Scholar
  8. Harris, C. C., Yolken, R. H., Krokan, H., Hsu, I. C., 1979, Ultrasensitive enzymatic radioimmunoassay: Application to detection of cholera toxin and rotavirus, Proc. Natl.Acad. Sci.USA 76:5336.PubMedCrossRefGoogle Scholar
  9. Hemmila, I., Dakubu, S., Mukkala, V.-M., Siitari, H., Lovgren, T., 1983, Europium as a label in time-resolved immunofluorometric assays, Anal.Biochem., 137:335.CrossRefGoogle Scholar
  10. Janata, J., Blackburn, G. F., 1984, Immunochemical potentiometric sensors, Ann.NY Acad. Sci., 286.Google Scholar
  11. Janata, J., Huber, R. J., 1980, Chemically sensitive field effect transisters, in: “lon-sensitive Electrodes in Analytical Chemistry,” H. Freiser ed, Plenum Press, Vol 2:107.Google Scholar
  12. Kohler, G., Milstein, C., 1975, Continuous culture of fused cells secreting specific antibody, Nature, 256:495.PubMedCrossRefGoogle Scholar
  13. McCapra, F., Tutt, D. E., Topping, R. M., 1977, Assay method utilizing chemiluminescence. British Patent no. 1, 461,877.Google Scholar
  14. McGown L. B., Bright, F. V., 1984, Phase-resolved fluorescence spectroscopy, Anal.Chem., 56:1400.Google Scholar
  15. North, J. R., 1985, Immunosensors: antibody-based biosensors, Trends in Biotechnology, 3/7:180.CrossRefGoogle Scholar
  16. Roederer, J. E., Bastiaans, G. J., 1983, Microgravimetric immunoasay with piezo-electric crystals, Anal.Chem., 55:2333.CrossRefGoogle Scholar
  17. Shalev, A., Greenberg, G. H., McAlpine, P. J., 1980, Detection of attograms of antigen by a high sensitivity enzyme-linked immunosorbent assay (HS-ELISA) using a fluorogenic substrate, J. Immunol. Methods., 38:125.PubMedCrossRefGoogle Scholar
  18. Stanley, C. J., Paris, F., Plumb, A., Webb, A., Johannsson, A., 1985, Enzyme amplification: A new technique for enhancing the speed and sensitivity of enzyme immunoassays, Int.Clin.Products Review July/ August 1985, 44.Google Scholar
  19. Sutherland, R. M., Dahne, C, Place, J. F., Ringrose, A. R., 1984, Immunoassays at a quartz-liquid interface: theory, instrumentation and preliminary application to the fluorescent immunoassay of human immunoglobulin G., J. Immunol. Methods., 74:253.PubMedCrossRefGoogle Scholar
  20. Weeks, I., Campbell, A. K., Woodhead, S., McCapra, F., 1984, Immunoassays using chemiluminescent labels in: “Practical Immunoassay. The State of the Art,” W.R. Butt, ed., Marcel Dekker, Inc. p. 103, New York.Google Scholar
  21. Whitehead, T. P., Thorpe, G. H., Carter, T. J., Groucutt, C., Kricka, L. J., 1983, Enhanced luminescence procedure for sensitive determination of peroxidase-labelled conjugates in immunoassay, Nature, 305:158.CrossRefGoogle Scholar
  22. Yamamoto, N., Yoshikatsu, N., Sadanobu, S., Tsubomura, H., Sawai, M., Okumura, H., 1980, Antigen-antibody reaction investigated with use of a chemically modified electrode, Clin.Chem., 26:1539.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Roger Ekins
    • 1
  1. 1.Department of Molecular EndocrinologyUniversity College and Middlesex School of Medicine, University of LondonLondonUK

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