Methods and Labels in Immunoassay

  • Roger Ekins


Immunoassay methods relying on radioisotopic labels have played a major role in medicine and other biologically-related fields (agriculture, veterinary science, the food industry etc) during the past two decades. Their importance has derived from their exploitation both of the “structural specificity” characterizing antibody-antigen reactions and the “detectability” of isotopically-labeled reagents, the latter permitting observation of the binding reactions between exceedingly small concentrations of the key reactants involved. The combination of these features has endowed these methods with unique specificity and sensitivity characteristics, and accounts for their ubiquitous use throughout modern medicine and biology. However, in the past few years, interest has increasingly focussed on so-called “alternative”, non-radioisotopic, methods — techniques which are based on essentially the same analytical principles but which differ in the markers used to label the particular reactant (antibody or analyte) whose distribution following the basic analytical reaction constitutes the assay response.


Analyte Molecule Antibody Molecule Excess Reagent Antibody Binding Site Small Molecular Size 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Roger Ekins
    • 1
  1. 1.Department of Molecular EndocrinologyUniversity College and Middlesex School of Medicine, University of LondonLondonUK

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