Monoclonal Antibodies to Gram-Negative Bacteria

  • Colin M. M. Stern


The need to identify and classify micro-organisms, either out of scientific curiosity, clinical need or industrial necessity, has been a major pre-occupation of bacteriologists and clinicians. Many methods have been used successfully for these purposes, although the utilization of the exquisite specificity of the immune response has often given valuable results. The immune response has evolved adaptively in the face of environmental pressure exerted, in part, by micro-organisms and one arm of this response rests upon the production of antibody. However, the polyclonality of the immunoglobulins generated during an immune reaction rendered the production of specific immune reagents a tedious process of cross-absorption.


Neisseria Meningitidis Clostridium Botulinum Yersinia Pestis Francisella Tularensis Brucella Abortus 
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  1. Carlin, N. I. A., and Lindberg, A. A., 1985, Monoclonal antibodies specific for the O-antigen of Shigella flexneri and Shigella sonnei: immunochemistry, characterization and clinical usefulness,“ in: “Monoclonal Antibodies against Bacteria, A.J. Macario and E.C. de Macario, pp. 146–54, Academic Press.Google Scholar
  2. de Macario, E. C, and Macario, A. J., 1986, “Monoclonal antibodies for bacterial identification and taxonomy,” ASM News, 49:1.Google Scholar
  3. Edberg, S. C., 1983, Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases, Yale J. Biol. Med, 58:425.Google Scholar
  4. Frank, D. W., and Parker, C.D., 1984, Interactions of monoclonal antibodies with pertussis toxin and its sub-units,” Inf and Immunity 46:195.Google Scholar
  5. Gabay, J., Schenckman, S., Desaymard, C, and Schwarz, M., 1985, Monoclonal antibodies and the structure of bacterial membrane proteins, in: “Monoclonal Antibodies against Bacteria,” A.J. Macario, and E.C. de Macario. pp. 249–82, Academic Press.Google Scholar
  6. Gigliotti, F., Stokes, D. C, Cheatham, A. B., Davis, D. S., and Hughes, W. T., 1986, Development of murine monoclonal antibodies to Pneumocystis carinii,” J. Infect.Dis., 154:315.PubMedCrossRefGoogle Scholar
  7. Gmur R., and Wyss, C., 1985, Monoclonal antibodies to characterize the antigenic heterogeneity of bacteroides intermedium, in: “Monoclonal Antibodies Against Bacteria,” A.J. Macario and E.C. de Macario, eds., pp. 210–16, Academic Press.Google Scholar
  8. Heelels, J. E., and Viji, M., 1985, “Monoclonal antibody against gonococcal pili: use in the analysis of gonococcal immunogenicity and virulence,” in; “Monoclonal Antibodies Against Bacteria,” A.J. Macario and E.C. de Macario, eds., pp. 91–103, Academic Press.Google Scholar
  9. Holman, P. J., Schwing, G., and Douglas, J. T., 1985, “Development of monoclonal antibodies to Brucella cell surface antigens, in: “Monoclonal Antibodies against Bacteria,” A.J. Macario and E.C. de Macario, pp. 82–110, Academic Press.Google Scholar
  10. Holme, T., and Gusterson, B., 1985, Monoclonal antibodies against group and type specific antigens of Vibrio Choleri O:I, in: “Monoclonal Antibodies against Bacteria, A.J. Macario and E.C. de Macario, ed., pp. 186–191, Academic Press.Google Scholar
  11. Hyypia, T., Jalava, A., Larsen, S., Terho, P., and Hukkanen, V., 1985, “Detection of Chlamydia trachomatis in clinical specimens by nucleic acid spot hybridization, J.Gen.Microbiol, 131:975.Google Scholar
  12. Joly, J. R., McKinney, R. M., and Watkins, I. D., 1985, “Monoclonal antibodies to Legionella pneumophila: possible applications in clinical diagnostic tests and epidemiological investigations, in: “Monoclonal antibodies against bacteria,” A.J. Macario and E.C de Macario, eds, pp. 112–130, Academic Press.Google Scholar
  13. Kamata, Y., Kozaki, S., Nagai, T. and Sakaguchi, G., 1985, Production of monoclonal antibodies against Clostridium botulinum type E derivative toxin, FEMS Microbiology Letts., 26:305.CrossRefGoogle Scholar
  14. Lana, J. S., Mutharia, L. M., and Hancock, R. E. W., 1985, Application of monoclonal antibodies to the study of the surface antigens of pseudomonas aeruginosa,” in: “Monoclonal Antibodies against Bacteria.” A.J. Macario and E.C. de Macario, pp. 143–157, Academic Press.Google Scholar
  15. Mitchison, D. A., and Coates, A. R. M., 1985, Monoclonal antibodies in bacteriology, in: “Monoclonal Antibodies in Clinical Medicine,” A.J. McMichael and J.W. Fabre, eds., pp. 305–312, Academic Press.Google Scholar
  16. Mutharia, L. M., Lana, J. S., and Hancock, R. E. W., 1985, Use of monoclonal antibodies in the study of various antigens of Gram-negative bacteria, in: “Monoclonal Antibodies against Bacteria,” A.J. Macario and E.C. de Macario, eds., pp. 131–142, Academic Press.Google Scholar
  17. Norgard, M. V., Marchitto, K. S., and Cox, D. L., 1986, Phenotypic expression of the major 47 kiloDalton surface immunogen of Treponema pallidum in virulent tissue-cultured treponemes, J.Gen.Microbiol., 132:1775.PubMedGoogle Scholar
  18. Oguma, K., Syuto, B., Kubo, S., and Hiroo, T., 1985, Analysis of antigenic structure of Clostridium botulinum type CI and D toxins by monoclonal antibodies, in: Monoclonal Antibodies Against Bacteria,” A.J. Marcario, and E.C. de Macario, eds. pp. 159–184, Academic Press.Google Scholar
  19. Okada, K., Mitsuyama, M., Miyake, S., and Amato, K., 1987, Monoclonal antibodies against the haemolysin of Vibrio vulnificus, J.Gen.Microbiol., 133:2279.PubMedGoogle Scholar
  20. Sethi, K. K., 1985, Monoclonal antibodies against legionella pneumophila serogroup I antigens: characterization and their potential applications, in: “Monoclonal Antibodies against Bacteria,” A.J. Macario and E.C. de Macario, eds., pp. 217–227, Academic Press.Google Scholar
  21. Sheppard, A. J., Church, D., and Hughes, M., 1984, Production and characterization of monoclonal antibodies to tetanus toxin, Inf and Immunity, 43:710.Google Scholar
  22. Soderstrom, T., 1985, Escherichia coli capsule and pili: serological, functional, protective and immunoregulatory studies with monoclonal antibodies, in: “Monoclonal Antibodies Against Bacteria,” A.J. Macario and E.C. de Macario, eds., pp. 185–212, Academic Press.Google Scholar
  23. Sugasawara, R. J., 1985, The use of monoclonal antibodies for detecting and serotyping Neisseria meningitidis, in: “Monoclonal Antibodies Against Bacteria, A.J. Macario and E.C. de Macario, eds. pp. 61–91, Academic Press.Google Scholar
  24. Zumein, D. H., Munder, F. I. L., and O’Neill, S. P., 1985, Industrial application of monoclonal antibodies against bacteria, in: “Monoclonal Antibodies Against Bacteria,” A.J. Macario and E. C., de Macario, eds., pp. 283–320, Academic Press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Colin M. M. Stern
    • 1
  1. 1.St. Thomas’s HospitalLondonUK

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