Cultures of Erythroleukemic Cells (K-562) on a Stratospheric Balloon Flight

  • C. Wiese
  • B. Bechler
  • G. Lorenzi
  • A. Cogoli
Chapter
Part of the Nato ASI Series book series (NSSA, volume 154)

Abstract

Our interest in the effects of cosmic radiation is directed towards understanding the behavior of cells in vitro rather than towards its possible consequences on man. The human cell line K-562 can be induced in culture to express erythroid-specific markers by addition of hemin. Growth, metabolism and differentiation of K-562 cells were measured. The most striking result was that hemoglobin biosynthesis was markedly depressed in flight. However, cell growth was not significantly altered while glucose consumption was increased in the flight samples. Although the results presented here are derived from one single experiment, it appears that there is a marked effect of cosmic radiation on K-562 cells in vitro.

Keywords

Lactate Leukemia Glutamine HEPES Erythropoietin 

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • C. Wiese
    • 1
  • B. Bechler
    • 1
  • G. Lorenzi
    • 1
  • A. Cogoli
    • 1
  1. 1.Laboratorium für BiochemieETH ZentrumZürichSwitzerland

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