Summary
Chinese Hamster (V79) cells were assayed for cell survival as measured by colony forming ability and for mutation at the hypoxanthine guanine phosphoribosyl transferase (HGPRT) locus as measured by resistance to 6-thioguanine (6-TG) after exposure to 160 MeV protons delivered at low dose rates at the Harvard University Cyclotron. Proton irradiations were carried out at three dose rates; 20, 0.5 and 0.1 rad/min. Preliminary results indicate that no difference exists between these radiations when cell survival was examined, but that low doses of protons delivered at low dose rates were more effective than higher doses in inducing mutations at the HGPRT locus. Indeed our initial results showed a peak in induced mutations at a dose of 5 rad delivered at a dose rate of 0.1 rad/min. When the proton dose was delivered at a dose rate of 20 rad/min. a dose of 40 rad was required to equal the mutation frequency observed for 5 rad delivered at 0.1 rad/min. This enhanced effect for low doses delivered at a low dose rate is similar to that reported by Hill et al. (1982) for transformation of 10T 1/2 cells by low doses of neutrons delivered at low dose rates.
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References
Hill, C.K., Buonaguro, F.M., Myers, C.P., Han, A. and Elkind, M.M., “Fission-spectrum neutron at reduced dose rates enhance neoplastic transformation”, Nature 298: 67–69, 1982.
Little, J.B., Nagasawa, H. and Kennedy, A.R. DNA Repair and Malignant Transformation: Effect of X-Irradiation, 12-0 Tetradecanoyl-phorbal-13-acetate and Protease Inhibitors on Transformation and Sister-Chromatid Exchanges in Mouse 10T1/2 Cells. Radiation Research 19: 241–255, 1979.
Nagasawa, H., Robertson, J.B. and Little, J.B. Induction of Chromosome Aberrations and Sister Chromatid Exchanges by X-ray and Alpha Particles in Density-Inhibited Cultures of Mouse 10T1/2 and 3T3 Cells. Environmental Mutagenesis 4: 376–377, 1982.
Robertson, J.B., Koehler, A., George, J. and Little, J.B. Oncogenic transformation of mouse BALB/3T3 cells by plutonium-238 alpha particles. Radiat. Res. 96: 261–274, 1983.
Thomson, J.F., Williamson, F.S., Grahn, D and Ainsworth, E.J. Life shortening in mice exposed to fission neutrons and rays. I. Single and short-term fractionated exposures. Radiat. Res. 86: 559–572, 1981.
Ullrich, R.L. and Storer, J.B. Life shortening and tumor induction after low doses of fission neutrons. Radiat. Res. 87: 454, 1981.
Vogel, H.H. Jr. and Dickson, H.W. Mammary neoplasia following acute and protracted irradiation with fission neutrons and cobalt gamma rays. Radiat. Res. 87: 453–454, 1981.
Wilson, R.R. Radiological use of fast protons. Radiology 47: 487–491, 1946.
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© 1988 Plenum Press, New York
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Robertson, J.B., Koehler, A.M., Weideman, P.A., McNulty, P.J. (1988). High Energy Proton Induced Mutations in Cultured Chinese Hamster Cells. In: McCormack, P.D., Swenberg, C.E., Bücker, H. (eds) Terrestrial Space Radiation and Its Biological Effects. Nato ASI Series, vol 154. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1567-4_23
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DOI: https://doi.org/10.1007/978-1-4613-1567-4_23
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