Abstract
The question on the nature of radiation induced mutations arose at the very early days of radiation genetics. According to H.J. Muller (1954) who founded the radiation genetics, working with the fruitfly Drosophila “it was evident practically from the start that gene mutations were being produced by ionizing radiation”. With the term gene mutation Muller meant permanent heritable changes in individual genes, which is quite close to what in our days we call intragenic mutations including the true point mutations i.e. changes of single nucleotides in the DNA molecule. On the other hand Stadler (1932) working independently with maize was led to the conclusion that ionizing radiation, and particularly X-rays, induce mainly chromosome aberrations that is mainly multigene deficiencies or other types of chromosome aberrations. The work of Stadler and his associates led to the conclusion that the great majority if not all of the induced mutations by X-rays in maize and probably other cereals were structural chromosome changes and not intragenic events.
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Literature
Auerbach, C, and Kibley, B., 1971, Mutations in eukaryotes, Ann. Rev. Genet., 5:163.
Breimer, L.H., Nalbantoglu, J., and Meuth, M., 1986, Structure and sequence of mutations induced by ionizing radiation on selectable loci in Chinese hamster ovary cells, J. Mol. Biol., 192:669.
Carrano, A., and Heddle, J., 1973, The fate of chromosome aberrations, J. Theor. Biol. 38:289.
Chu, E.H.Y. and Mailing, H.V., 1968, Mammalian cells genetics II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Natl. Acad. Sci., 61:1306.
Chu, E.H.Y., 1971, Mammalian cell genetics III. Characterization of X-ray induced forward mutations in Chinese hamster cell cultures. Mutation Res., 11:23.
Cox, R., 1979, Comparative mutagenesis in cultured mammalian cells. In M. Alacevic (ed.) Progress in Environmental Mutagenesis, Elsevier Publ., vol. 7:33.
Cox, R. and Masson, W.K., 1978, Do radiation-induced thiognanine resistant mutants of cultured mammalian cells arise by HG-PRT gene mutation or X-chromosome rearrangements. Nature 276:629.
De Ruijter, Y.C.E.M. and Simons, J.W.I.M., 1980, Determination of the expression time and the dose response relationship for mutation of the HGRPT (Hypoxanthine guanine phosphorbosyl ransferase) locus induced by X-irradiation in human diploid stain fibroblasts. Mutation Res. 69:325.
Duin, M., van, de Wit, J., Odijk, H., Westerveld, A., Yasui, A., Koken, M.H.M., Hoeijmakers, J.H.J, and Bootsma, D., 1986, Molecular characterization of the human excision repair gene ERCC-1. Cell, 44:913.
Evans, H.J. and Vijayalaxmi, 1981, Induction of 8-azaguanine resistance and sister chromatid exchange in lymphocytes exposed to mitomycin C and X rays in vitro. Nature, 282:601.
Freese, E., Reese, H.J. and Bautz Freeze E., 1969, Chemical DNA alterations causing inactivation and chromosome breaks. Japan. J. Genetics, 44, Suppl. 1:94.
Fuscoe, J.C., Ockey, C.H., and Fox, M., 1986, Molecular analysis of X-ray induced mutants at the HPRT locus in V72 Chinese hamster cells. Int. J. Radiat. Biol., 49:1011.
Grosovsky, A.J., Drobetsky, E.A., de Jong, P.J., and Glickman, B.W., 1986. Southern analysis of genomic alterations in gamma-ray-induced APRThamster cell mutants. Genetics, 113:405.
Hutchinson, F., 1985, Chemical changes induced in DNA by ionizing radiation, Progr. Nucl. Acid. Res. Mol. Biol., 32:115.
Kleinhofs, A., Shunway, L.K. and Sideris, E.G., 1969, Biochemical and ultrastructural characterization of chlorophyll-deficient barley mutants. Proc. II. Intern. Symp. of Barley Genetics, Pp 194–200.
Latarjet, R., Eckert, B. and Demerseman, P., 1963, Peroxidation of nucleic acids by radiation: Biological implications. Radiat. Res. Suppl., 3:247.
Lifschytz, E., and R. Falk, 1969, Fine structure analysis of a chromosome segment in Drosophile melanogaster. Analysis of X-ray induced mutants. Mutation Res., 6:235–244.
Lyon, M.F., Phillips, R.J.S., and Fisher, S., 1979, Dose response curves for radiation induced gene mutations in mouse oocytes and their interpretation, Mutation Res., 63:161.
Montiger, J.P., 1970, The effect of X-rays on the bronze and shrunken loci in maize, Genetics, 61:259.
Muller, H.J., 1954, The nature of the genetic effects produced by irradiation, In: A. Hollander (ed.), Radiation Biology, 1:313.
Muller, H.J., 1955, On the relation between chromosome changes and gene mutation, Brookhaven Symposia in Biology, 8:126.
Muller, H.J., Valencia, R.M., and Valencia, J.I., 1950a, The production of mutations at individual loci in Drosophila by irradiation of oocytes and oogonia. Genetics, 34:126.
Muller, H.J., Valencia, R.M., and Valencia, J.I., 1950b., The frequency of spontaneous mutations at individual loci in Drosophile. Genetics, 35:125.
Nilan, R.A., Kleinhofs, A. and Sideris, E.G., 1969, Structural and biochemical concepts of mutations in flowering plants. In “Induced Mutations in Plants”, IAEA, Vienna Pp 35–49.
Petrakis, H., and Sideris, E.G., 1982, Evidence from mutagenesis on the possible involvement of Cu++ and Mg++ ions in the eukaryotic chromosome structure, Studia Biophys., 92:1.
Russell, L.B., 1971, Definition of functional units in a small chromosomal segment of the mouse and its use in interpreting the nature of radiation induced mutations, Mutation Res., 11:107.
Russell, L.B., Montgomery, C.S., and Raymer, G.D., 1982, Analysis of the albino locus region of the mouse IV. Characterization of 34 deficiencies, Genetics, 100:427.
Russell, W.L., 1965, Evidence for mice concerning the nature of the mutation process, Proc. 11th Congr. Genetics, vol. 2, Pp. 257–264.
Russell, W.L., 1972, The genetic effects of radiation, In: Peaceful Uses of Atomic Energy, vol. 13, Pp. 487–500.
Russell, W.L., 1977, Mutation frequencies in female mice and the estimation of genetic hazards of radiation in women. Proc. Natl. Acad. Sci., 74:3523.
Russell, W.L., Russell, L.B. and Kelly, E.M., 1959, Radiation dose rate and mutation frequency, Science, 128:1546.
Sankaranarayanan, K., 1982, Genetic effects of ionizing radiation in multicellular eukaryotes and the assessment of genetic radiation hazards in man. Elsevier Biomedical Press.
Serres, de F.I., and Mailing, H.V., 1969, Identification of the genetic alterations in specific locus mutants at the molecular level, Japan J. Genetics, 44, Suppl. 1:106.
Sideris, E.G., Nilan, R.A., and Bogyo, T.P., 1973, Differential effect of sodium azide on the frequency of radiation induced chromosome aberrations vs the frequency of radiation induced chlorophyll mutation in Hordeum vulgare. Radiation Botany, 13:315.
Sideris, E.G., Nilan, R.A., and Konzak, C.F., 1969, Relationship of radiation induced damage in barley seeds to the inhibition of certain oxidoreductases by sodium azide. In “Induced Mutations in Plants”, IAEA, Vienna, Pp. 313–321.
Stadler, L., 1932, On the genetic nature of induced mutations in plants. Proc. 6gh Intern. Congr. Genet., Vol. 1, Pp. 274–294.
Stankowski, L.F., and Hsie, A.W., 198b, Quantitative and molecular analyses of radiation-induced mutation in A552 Cells, Radiat. Res., 105:37.
Thacker, J., 1986, The use of recombinant DNA techniques to study radiation induced damage repair and genetic change in mammalian cells. Intern. J. Radiat. Biol., 50:229.
Thacker, J., 1986, The nature of mutants induced by ionizing radiation in cultured hamster cells, Mutat. Res., 160:267.
U.N.S.C.E.A.R. Committee Report, 1982, Ionizing Sources and Biological Effects, United Nations.
Ward, C.L., and Alexander, M.L., 1957, Cytological analysis of X-ray induced mutations at eight specific loci in the third chromosome of Drosophila melanogaster. Genetics, 42:42.
Webber, B.B. and de Serres, F.J., 1965, Induction kinetics and genetic analysis of X-ray induced mutations in the ad-3 region of Neurospora crassa, Proc. Natl. Acad. Sci., 53:430.
Westerveld, A., Visser, R.P., Freeke, M.A., and Bootsna, D., 1972, Evidence for linkage of 3-phosphoglyceryl kinase, hypoxanthine guanine phosphoribosyl transferase and glucose-6-phosphate dehydrogenase loci in Chinese hamster cells studied using a relationship between gene multiplicity and enzyme activity, Biochem. Genet. 7:33.
Wolf, S., 1967, Radiation Genetics, Ann. Rev. Genet., 1:221.
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© 1988 Plenum Press, New York
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Sideris, E.G. (1988). Nature of Radiation Induced Mutations: Experimental Approaches on the Question of Intragenic Events. In: McCormack, P.D., Swenberg, C.E., Bücker, H. (eds) Terrestrial Space Radiation and Its Biological Effects. Nato ASI Series, vol 154. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1567-4_18
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