Anti-antibody enhancement of tumor imaging

  • Robert M. Sharkey
  • Rosalyn D. Blumenthal
  • David M. Goldenberg
Part of the Cancer Treatment and Research book series (CTAR, volume 51)

Abstract

Although there are many factors that influence the ability of a radiolabeled antibody to image tumors by external scintigraphy, perhaps the most problematic has been the ability to distinguish specific radioantibody uptake in tumor from the background radioactivity in uninvolved tissues, particularly that due to excessive blood-pool activity. This problem was recognized even as the technology was first developing in the mid-1950s [1], but it was thought that the development of more highly specific anti-tumor antibodies would be a solution. However, even as specificity of the antibodies was improved, tumor imaging was difficult. For example, in animals bearing human tumor xenografts in peripheral sites, such as the cheek pouch in hamsters or subcutaneously in nude mice, tumors were not clearly imaged until 4–7 days after the radioantibody was injected [2–4], because it took several days for the blood-pool radioactivity to clear. Otherwise, the majority of the counts in the images was derived from radioactivity residing in the blood and uninvolved tissues. Excessive background radioactivity also interfered with the detection of tumors in humans [5], but this problem was overcome initially by Golden-berg et al. [6]. They developed a dual-isotope subtraction procedure that used 99mTc-pertechnetate and labeled albumin to estimate the contribution of interstitial and blood-pool radioactivity in the images produced.

Keywords

Permeability Toxicity Filtration Albumin Attenuation 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Robert M. Sharkey
  • Rosalyn D. Blumenthal
  • David M. Goldenberg

There are no affiliations available

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