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Decreased expression of cardiac sarcoplasmic reticulum Ca2+-pump ATPase in congestive heart failure due to myocardial infarction

  • Angel Zarain-Herzberg
  • Nasir Afzal
  • Vijayan Elimban
  • Naranjan S. Elimban
Chapter
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 19)

Abstract

Myocardial infarction in rats induced by occluding the left coronary artery for 4, 8 and 16 weeks has been shown to result in congestive heart failure (CHF) characterized by hypertrophy of the viable ventricular myocardial tissue. We have previously demonstrated a decreased calcium transport activity in the sarcoplasmic reticulum (SR) of post-myocardial infarction failing rat hearts. In this study we have measured the steady state levels of the cardiac SR Ca2+-pump ATPase (SERCA2) mRNA using Northern blot and slot blot analyses. The relative amounts of SERCA2 mRNA were decreased with respect to GAPDH mRNA and 28 S rRNA in experimental failing hearts at 4 and 8 weeks post myocardial infarction by about 20% whereas those at 16 weeks declined by about 35% of control values. The results obtained by Western blot analysis, revealed that the immunodetectable levels of SERCA2 protein in 8 and 16 weeks postinfarcted animals were decreased by about 20% and 30%, respectively. The left ventricular SR Ca2+-pump ATPase specific activity was depressed in the SR preparations of failing hearts as early as 4 weeks post myocardial infarction and declined by about 65% at 16 weeks compared to control. These results indicate that the depressed SR Ca2+-pump ATPase activity in CHF may partly be due to decreased steady state amounts of SERCA2 mRNA and SERCA2 protein in the failing myocardium.

Key words

Ca2+-pump ATPase cardiac SERCA2 myocardial infarction congestive heart failure cardiac SR 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Angel Zarain-Herzberg
    • 1
    • 2
  • Nasir Afzal
    • 1
    • 2
  • Vijayan Elimban
    • 1
    • 2
  • Naranjan S. Elimban
    • 1
    • 2
  1. 1.Division of Cardiovascular SciencesSt. Boniface General Hospital Research CentreWinnipegCanada
  2. 2.Department of Physiology, Faculty of MedicineUniversity of ManitobaWinnipegCanada

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