Decreased expression of cardiac sarcoplasmic reticulum Ca2+-pump ATPase in congestive heart failure due to myocardial infarction

  • Angel Zarain-Herzberg
  • Nasir Afzal
  • Vijayan Elimban
  • Naranjan S. Elimban
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 19)


Myocardial infarction in rats induced by occluding the left coronary artery for 4, 8 and 16 weeks has been shown to result in congestive heart failure (CHF) characterized by hypertrophy of the viable ventricular myocardial tissue. We have previously demonstrated a decreased calcium transport activity in the sarcoplasmic reticulum (SR) of post-myocardial infarction failing rat hearts. In this study we have measured the steady state levels of the cardiac SR Ca2+-pump ATPase (SERCA2) mRNA using Northern blot and slot blot analyses. The relative amounts of SERCA2 mRNA were decreased with respect to GAPDH mRNA and 28 S rRNA in experimental failing hearts at 4 and 8 weeks post myocardial infarction by about 20% whereas those at 16 weeks declined by about 35% of control values. The results obtained by Western blot analysis, revealed that the immunodetectable levels of SERCA2 protein in 8 and 16 weeks postinfarcted animals were decreased by about 20% and 30%, respectively. The left ventricular SR Ca2+-pump ATPase specific activity was depressed in the SR preparations of failing hearts as early as 4 weeks post myocardial infarction and declined by about 65% at 16 weeks compared to control. These results indicate that the depressed SR Ca2+-pump ATPase activity in CHF may partly be due to decreased steady state amounts of SERCA2 mRNA and SERCA2 protein in the failing myocardium.

Key words

Ca2+-pump ATPase cardiac SERCA2 myocardial infarction congestive heart failure cardiac SR 


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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Angel Zarain-Herzberg
    • 1
    • 2
  • Nasir Afzal
    • 1
    • 2
  • Vijayan Elimban
    • 1
    • 2
  • Naranjan S. Elimban
    • 1
    • 2
  1. 1.Division of Cardiovascular SciencesSt. Boniface General Hospital Research CentreWinnipegCanada
  2. 2.Department of Physiology, Faculty of MedicineUniversity of ManitobaWinnipegCanada

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