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Phosphotransfer reactions as a means of G protein activation

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Biochemistry of Signal Transduction in Myocardium

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 17))

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Abstract

Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) serve to transduce information from agonist-bound receptors to effector enzymes or ion channels. Current models of G protein activation-deactivation indicate that the oligomeric GDP-bound form must undergo release of GDP, bind GTP and undergo subunit dissociation, in order to be in active form (GTP bound α subunits and free βγ dimers) and to regulate effectors. The effect of receptor occupation by an agonist is generally accepted to be promotion of guanine nucleotide exchange thus allowing activation of the G protein. Recent studies indicate that transphosphorylation leading to the formation of GTP from GDP and ATP in the close vicinity, or even at the G protein, catalysed by membrane-associated nucleoside diphosphate kinase, may further activate G proteins. This activation is demonstrated by a decreased affinity of G protein-coupled receptors for agonists and an increased response of G protein coupled effectors. In addition, a phosphorylation of G protein β subunits and consequent phosphate transfer reaction resulting in G protein activation has also been demonstrated. Finally, endogenously formed GTP was preferentially effective in activating some G proteins compared to exogenous GTP. The aim of this report is to present an overview of the evidence to date for a transphosphorylation as a means of G protein activation (see also refs [1 and 2] for reviews).

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Authors and Affiliations

  1. Innere Medizin III - Kardiologie, Universität Heidelberg, Bergheimer Str. 58, D-69115, Heidelberg, Germany

    Lucia Piacentini & Feraydoon Niroomand

Authors
  1. Lucia Piacentini
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  2. Feraydoon Niroomand
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Editors and Affiliations

  1. Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine of Health Science, Erasmus University Rotterdam, P.O. Box 1738, 3300 DR, Rotterdam, The Netherlands

    Jos M. J. Lamers

  2. Department of Cardiology, Cardiovascular Research Institute COEUR, Erasmus University Rotterdam, P.O. Box 1738, 3300 DR, Rotterdam, The Netherlands

    Pieter D. Verdouw

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© 1996 Kluwer Academic Publishers

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Piacentini, L., Niroomand, F. (1996). Phosphotransfer reactions as a means of G protein activation. In: Lamers, J.M.J., Verdouw, P.D. (eds) Biochemistry of Signal Transduction in Myocardium. Developments in Molecular and Cellular Biochemistry, vol 17. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1275-8_7

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  • DOI: https://doi.org/10.1007/978-1-4613-1275-8_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8544-1

  • Online ISBN: 978-1-4613-1275-8

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