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β-Adrenoceptor mediated signal transduction in congestive heart failure in cardiomyopathic (UM-X7.1) hamsters

  • Deepak Kaura
  • Nobuakira Takeda
  • Rajat Sethi
  • Xi Wang
  • Makoto Nagano
  • Naranjan S. Dhalla
Chapter
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 17)

Abstract

In view of the lack of information regarding the status of β-adrenoceptor mediated signal transduction mechanisms at severe stages of congestive heart failure, the status of β-adrenoceptors, G-proteins and adenylyl cyclase activities was examined in 220–275 day old cardiomyopathic hamster hearts. Although no changes in the Kd values for β1- and β2-adrenoceptors were seen, the number of β1-adrenoceptors, unlike that of β2-adrenoceptors, was markedly decreased in cardiac membranes from failing hearts. The activation of adenylyl cyclase in the failing hearts by different concentrations of isoproterenol was also attenuated in comparison to the control preparations. The basal adenylyl cyclase activity in cardiac membranes from the failing hearts was not altered; however, the stimulated enzyme activities, when measured in the presence of forskolin, NaF or Gpp(NH)p were depressed significantly. The functional activity of Gs-proteins (measured by cholera toxin stimulation of adenylyl cyclase) was depressed whereas that of Gi-proteins (measured by pertussis toxin stimulation of adenylyl cyclase) was increased in the failing hearts. Not only were the Gs- and Gi-protein contents (measured by immunoblotting) increased, the bioactivities of these proteins as determined by ADP-ribosylations in the presence of cholera toxin and pertussis toxin, respectively, were also higher in failing hearts in comparison to the control values. Northern blot analysis revealed that the signals for Gs- and Gi-protein mRNAs were augmented at this stage of heart failure. These results indicate that the loss of adrenergic support at severe stages of congestive heart failure in cardiomyopathic hamsters may involve a reduction in the number of β1-adrenoceptors, and an increase in Gi-protein contents as well as bioactivities in addition to an uncoupling of Gs-proteins from the catalytic site of adenylyl cyclase in cardiac membrane.

Key words

cardiac β-adrenoceptors adenylyl cyclase in heart myocardial G-proteins signal transduction mechanisms congestive heart failure cardiomyopathic hamsters 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Deepak Kaura
    • 1
    • 2
  • Nobuakira Takeda
    • 3
  • Rajat Sethi
    • 1
    • 2
  • Xi Wang
    • 1
    • 2
  • Makoto Nagano
    • 3
  • Naranjan S. Dhalla
    • 1
    • 2
  1. 1.Division of Cardiovascular SciencesSt. Boniface General Hospital Research CentreWinnipegCanada
  2. 2.Department of Physiology, Faculty of MedicineUniversity of ManitobaWinnipegCanada
  3. 3.Department of Internal Medicine, Aoto HospitalJikei UniversityTokyoJapan

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