Unique and selective mitogenic effects of vanadate on SV40-transformed cells

  • Hanlin Wang
  • Robert E. Scott
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 16)


Vanadate and insulin both function as unique complete mitogens for SV40-transformed 3T3T cells, designated CSV3-1, but not for nontransformed 3T3T cells. The mitogenic effects induced by vanadate and insulin in CSV3-1 cells are mediated by different signaling mechanisms. For example, vanadate does not stimulate the tyrosine phosphorylation of the insulin receptor β-subunit nor the 170 kDa insulin receptor substrate-1. Instead, vanadate induces a marked increase in tyrosine phosphorylation of 55 and 64 kDa proteins that is not observed in insulin-stimulated CSV3-1 cells. Perhaps most interestingly, vanadate-in-duced mitogenesis is associated with the selective induction of c-jun and junB expression without significantly inducing c-fos or c-myc. Furthermore, treatment of CSV3-1 cells with genistein abolishes the effects of vanadate on protein tyrosine phosphorylation and c-jun induction. These and related data suggest that modulation of protein tyrosine phosphorylation and c-jun and junB expression may serve the critical roles in mediating vanadate-induced mitogenesis in SV40-transformed cells.

Key words

adipocyte differentiation neoplastic transformation insulin c-jun junB tyrosine phosphorylation 


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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Hanlin Wang
    • 1
  • Robert E. Scott
    • 1
  1. 1.Department of PathologyThe University of Tennessee College of MedicineMemphisUSA

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