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Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex

  • Catharina Hiort
  • Jerry Goodisman
  • James C. Dabrowiak
Chapter
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 16)

Abstract

Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VO(O2)2-(1,10-phenanthroline)], bpV(phen), have been studied. 51V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.

Key words

DNA cleavage insulin-mimetic bisperoxovanadium photolysis photocleavage 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Catharina Hiort
    • 1
  • Jerry Goodisman
    • 1
  • James C. Dabrowiak
    • 1
  1. 1.Department of ChemistrySyracuse UniversitySyracuseUSA

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