The Use of EPR Spectroscopy for the Identification of the Nature of Endothelium-Derived Relaxing Factor

  • Yann A. Henry


The discovery by Furchgott and Zawadzki in 19801 of an obligatory entity derived from endothelial cells necessary for the relaxation of underlying arterial smooth muscle by acetylcholine was followed by the hypothesis made simultaneously by Furchgott and by Ignarro in 1986 that the endothelium-derived relaxing factor (EDRF) was in fact NO.2–5 This was immediately confirmed by Ignarro and Moncada’s groups,6–9 explaining the prime functional importance of the stimulation of guanylate cyclase by NO, earlier discovered by Murad et al and Ignarro et al.10–12 Vanhoutte questioned whether it was the end of the quest for EDRF.13 Read this thrilling part of scientific history recently related by Furchgott!14


Nitric Oxide Nitric Oxide Guanylate Cyclase Dinitrosyl Iron Dinitrosyl Iron Complex 
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  • Yann A. Henry

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