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Clinical Applications of Electroporation

  • S. B. Dev
  • G. A. Hofmann

Abstract

Electroporation is a well-established physical technique of introducing molecules, such as drugs, antibodies, or DNA, into cells by creating transient pores in the cell membrane. This is done by applying an electrical field to a suspension of cells with the transfectant (e.g., drugs) in a container with electrodes. Applications include preparation of gene libraries and genetic manipulation. Recently, however, the technology has gone beyond research laboratories and is being applied in clinical sciences. In this chapter applications are discussed both in the areas of drug delivery and of gene therapy. Examples include cancer, AIDS, restenosis, hemophilia B, and aplastic anemia, the last two nearing clinical trial. The advantages of electroporation over retrovirus-based gene therapy are discussed, and it is speculated that all single gene diseases with a genomic size less than 190 kb should be amenable to electroporetic gene therapy. Electroinsertion, the related technique of creating small pores to insert molecules in the surface of the cell membrane, may provide an effective way to overcome the current problems of delivering peptides and proteins.

Keywords

Gene Therapy Duchenne Muscular Dystrophy Aplastic Anemia Familial Hypercholesteremia Duchenne Muscular Dystrophy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Chapman & Hall 1996

Authors and Affiliations

  • S. B. Dev
  • G. A. Hofmann

There are no affiliations available

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