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Automated clean-up techniques for trace component analysis in complex biological matrices including foods

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Progress in Food Contaminant Analysis

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Summary

Chromatography-based techniques for automating trace component determination in biological matrices are examined. Once samples are brought into solution or extracted, a range of fluid handling systems may be applied to automate most analyses. However, extraction remains a limiting step and the development costs and time required to automate any method successfully must be carefully considered. These and other factors which will influence the decision to automate any analytical method are explored and the problems of trace analysis discussed. Methods based on solid-phase extraction, dialysis and column switching approaches are evaluated in detail and interesting approaches described.

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Abbreviations

AASP:

Advanced automated sample preparation

ASPEC:

Automated sample preparation by extraction and concentration

ASTED:

Automated sample treatment by extraction and dialysis

CAS:

Chemical Abstracts Service

CE:

Capillary electrophoresis

CV:

Coefficient of variation

ECD:

Electron capture detector

GPC:

Gel permeation chromatography

IAC:

Immunoaffinity chromatography

IR:

Infra-red

LC-GC:

Coupled liquid chromatography/gas chromatography

LC-LC:

Coupled liquid chromatography/liquid chromatography

LIMS:

Laboratory information management systems

MSTFA:

Monosilyltrifluoroacetamide (silylation derivatising reagent)

MTBE:

Methyl t-butyl ether

OC:

Organochlorine

PIONA:

Paraffin-iso/normal olefin—naphthalene—aromatic

SEC:

Size exclusion chromatography

SFE and SFC:

Supercritical fluid extraction and chromatography

SPE:

Solid-phase extraction

THF:

Tetrahydrofuran

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  1. Martin J. Shepherd
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Editors and Affiliations

  1. Ministry of Agriculture, Fisheries and Food, CSL Food Science Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UQ, UK

    J. Gilbert

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© 1996 Chapman & Hall

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Shepherd, M.J. (1996). Automated clean-up techniques for trace component analysis in complex biological matrices including foods. In: Gilbert, J. (eds) Progress in Food Contaminant Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1117-1_2

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  • DOI: https://doi.org/10.1007/978-1-4613-1117-1_2

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