Induction of the Proteinase Inhibitor α2-Macroglobulin in Rat Hepatocytes by a Monocyte-Derived Factor
Disturbances of the physiologic homeostasis such as infections, tissue injury, tumor growth and immunologic disorders lead to a highly complex reaction of the organism, the so called acute-phase response1-3. The acute-phase response is characterized by fever, leukocytosis, a negative nitrogen balance, depression of serum iron and zinc levels, elevation of serum copper and dramatic changes in the synthesis of hepatic acute-phase proteins4,5. The plasma concentration of the proteinase inhibator α2-macroglobulin (α2M) or example increases 100-500-fold in the rat during acute inflammation. The concentration of the proteinase-inhibitor α1-inhibitor3 (α1I3) belonging to the same macroglobulin family decreases to about 30% simultaneousely. These changes are generated by mediators secreted from mononuclear phagocytes. Hepatocyte-stimulating factor (HSF), interleukin 1 (IL-1), tumor necrosis factor α (TNFα) and interferon ß (IFN ß) are the most important of theses mediators.
KeywordsConditioned Medium Human Monocyte Negative Nitrogen Balance Monocyte Macrophage Monocyte Medium
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- 2.J. D. Sipe, Cellular and humoral components of the early inflammatory reaction, in: The Acute Phase Response to Injury and Infection, A. Koj, and A.H. Gordon, eds., p. 23, Elsevier, Amsterdam (1985).Google Scholar
- 3.J. J. Oppenheim, E.J. Kovacs, K. Matsushima, and S.K. Durum, 1986, There is more than one interleukin 1, Immunol. Today 7:45.Google Scholar
- 4.I. Kushner, 1982, The phenomenon of the acute-phase response, Ann. N. Y. Acad. Sci. 389:39.Google Scholar
- 5.G. Schreiber, and G. Howlett, in: Plasma Protein Secretion by the Liver, H. Glaumann, T. Peters, and C. Redman, eds., p. 423, Academic Press, London (1983).Google Scholar
- 8.J. Bauer, M. Birmelin, G.-H. Northoff, W. Northemann, T.-A. Tran-Thi, H. Ueberberg, K. Decker, and P.C. Heinrich, 1984, Induction of rat α2-macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell-derived factor, FEBS Lett. 177:89.PubMedCrossRefGoogle Scholar
- 9.H. Northoff, C. Carter, and J.J. Oppenheim, 1980, Inhibition of concanavalin A-induced human lymphocyte mitogenic factor (interleukin-2). Production by suppressor T-Iymphocytes. J. Immunol. 125:125.Google Scholar
- 10.C. A. Dinarello, L.J. Rosenwasser, and S.M. Wolff, 1981, Demonstration of a circulating suppressor factor of thymocyte proliferation during endotoxin fever in humans, J. Immunol. 127:2517.Google Scholar