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The Analysis of Chromosome Organization by Experimental Manipulation

  • Chapter
Chromosome Structure and Function

Part of the book series: Stadler Genetics Symposia Series ((SGSS))

Abstract

Chromosomes have occupied a pivotal position in genetics ever since their involvement in heredity became firmly established in the early years of the twentieth century. There has consequently been a longstanding and fervent desire to understand how chromosomes are organized, and in particular, to ascertain how the hereditary material is arranged and regulated at the molecular level.

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References

  • Abuelo, J. G., and Moore, D. E., 1969, The human chromosome. Electron microscopic observations on chromatin fiber organization, J. Cell Biol., 41: 73–90.

    PubMed  CAS  Google Scholar 

  • Adolph, K. W., 1980a, Organization of chromosomes in mitotic HeLa cells, Exp. Cell Res., 125: 95–103.

    PubMed  CAS  Google Scholar 

  • Adolph, K. W., 1980b, Isolation and structural organization of human mitotic chromosomes, Chromosoma, 76: 23–33.

    PubMed  CAS  Google Scholar 

  • Adolph, K. W., 1981, A serial sectioning study of the structure of human mitotic chromosomes, Eur. J. Cell Biol., 24: 146–153.

    PubMed  CAS  Google Scholar 

  • Adolph, K. W., Cheng, S. M., and Laemmli, U. K., 1977a, Role of nonhistone proteins in metaphase chromosome structure, Cell, 12: 805–816.

    PubMed  CAS  Google Scholar 

  • Adolph, K. W., Cheng, S. M., Paulson, J. R., and Laemmli, U. K., 1977b, Isolation of a protein scaffold from mitotic HeLa cell chromosomes, Proc. Natl. Acad. Sci. USA, 74: 4937–4941.

    CAS  Google Scholar 

  • Adolph, S., and Hameister, H., 1985, In situ nick translation of metaphase chromosomes with biotin-labeled d-UTP, Human Genet., 69: 117121.

    Google Scholar 

  • Anderson, L. K., Stack, S. M., and Mitchell, J. B., 1982, An investigation of the basis of a current hypothesis for the lack of G-banding in plant chromosomes, Exp. Cell Res., 138: 433–436.

    PubMed  CAS  Google Scholar 

  • Arrighi, F. E., and Hsu, T. C., 1971, Localization of heterochromatin in human chromosomes, Cytogenetics, 10: 81–86.

    PubMed  CAS  Google Scholar 

  • Arrighi, F. E., Hsu, T. C., Pathak, S., and Sawada, H., 1974, The sex chromosomes of the Chinese hamster: constitutive heterochromatin deficient in repetitive DNA sequences, Cytogenet. Cell Genet., 13: 268–274.

    PubMed  CAS  Google Scholar 

  • Bajer, A., and Mole-Bajer, J., 1957, Mitosis in Endosperm, I., 16 mm film.

    Google Scholar 

  • Bak, A. L., Zeuthen, J., and Crick, F. H. C., 1977, Higher-order structure of human mitotic chromosomes, Proc. Natl. Acad. Sci. USA, 74: 1595–1599.

    CAS  Google Scholar 

  • Benyajati, C., and Worcel, A., 1976, Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster, Cell, 9: 393–407.

    PubMed  CAS  Google Scholar 

  • Bianchi, M. S., Bianchi, N. O., Pantelias, G. E., and Wolff, S., 1985, The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes, Chromosoma, 91: 131–136.

    PubMed  CAS  Google Scholar 

  • Bobrow, M., and Madan, K., 1973, The effects of various banding procedures on human chromosomes, studied with acridine orange, Cytogenet. Cell Genet., 12: 145–156.

    CAS  Google Scholar 

  • Bosman, F. T., and Nakane, P. K., 1978, Immunoelectronmicroscopy of metaphase chromosomes, J. Histochem. Cytochem., 26: 217.

    Google Scholar 

  • Bostock, C. J., and Prescott, D. M., 1971, Buoyant density of DNA synthesized at different stages of the S phase of mouse L cells, Exp. Cell Res., 64: 267–274.

    PubMed  CAS  Google Scholar 

  • Brody, Th., 1974, Histones in cytological preparations, Exp. Cell Res., 85: 255–263.

    PubMed  CAS  Google Scholar 

  • Brown, R. L., Pathak, S., and Hsu, T. C., 1975, The possible role of histones in the mechanism of chromosomal G banding, Science, 189: 1090–1091.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., 1974, Electron microscopic visualization of chromosomes banded with trypsin, Nature, 247: 292–294.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., 1975, The ultrastructure of G- and C-banded chromosomes, Exp. Cell Res., 90: 269–278.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., 1981, The ultrastructure of R-banded chromosomes, Chromosoma, 83:473–480,

    PubMed  CAS  Google Scholar 

  • Burkholder, G, D., 1983, Silver staining of histone-depleted metaphase chromosomes, Exp. Cell Res., 147:287–296,

    PubMed  CAS  Google Scholar 

  • Burkholder, G, D., 1987, Endonuclease-induced chromosome banding: Structural and biochemical effects, in preparation.

    Google Scholar 

  • Burkholder, G, D., and Comings, D. E., 1972, Do the Giemsa-banding patterns of chromosomes change during embryonic development? Exp. Cell Res., 75: 268–271.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., and Weaver, M. G., 1977, DNA-protein interactions and chromosome banding, Exp. Cell Res., 110:251–262.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., and Duczek, L. L., 1980a, Proteins in chromosome banding. I. Effect of G-banding treatments on the proteins of isolated nuclei, Chromosoma, 79: 29–41.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., and Duczek, L. L., 1980b, Proteins in chromosome banding. II. Effect of R- and C-banding treatments on the proteins of isolated nuclei, Chromosoma, 79: 43–51.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., and Duczek, L. L., 1982a, The effect of the chromosome banding techniques on the histone and nonhistone proteins of isolated chromatin, Can. J. Biochem., 60: 328–337.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., and Duczek, L. L., 1982b, The effect of chromosome banding techniques on the proteins of isolated chromosomes, Chromosoma, 87: 425–435.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., and Kaiserman, M. Z., 1982, Electron microscopy of silver-stained core-like structures in metaphase chromosomes, Can. J. Genet. Cytol., 24: 193–199.

    PubMed  CAS  Google Scholar 

  • Burkholder, G. D., and Schmidt, G. J., 1986, Endonuclease banding of isolated mammalian metaphase chromosomes, Exp. Cell Res., 164: 379–387.

    PubMed  CAS  Google Scholar 

  • Bustin, M., Yamasaki, H., Goldblatt, D., Shani, M., Huberman, E., and Sachs, L., 1976, Histone distribution in chromosomes re-vealed by antihistone sera, Exp. Cell Res., 97:440–444.

    PubMed  CAS  Google Scholar 

  • Buys, C. H. C. M., and Osinga, J., 1980, Abundance of protein-bound sulfhydryl and disulfide groups at chromosomal nucleolus organizing regions. A cytochemical study on the selective silver staining of NORs, Chromosoma, 77: 1–11.

    PubMed  CAS  Google Scholar 

  • Caspersson, T., Zech, L., Johansson, C., and Modest, E. J., 1970, Identification of human chromosomes by DNA-binding fluorescent agents, Chromosoma, 30: 215–227.

    PubMed  CAS  Google Scholar 

  • Chandley, A. C., 1986, A model for effective pairing and recombination at meiosis based on early replicating sites (R-bands) along chromosomes, Human Genet., 72: 50–57.

    CAS  Google Scholar 

  • Cockerill, P. N., and Garrard, W. T., 1986, Chromosomal loop an-chorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites, Cell, 44: 273–282.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., 1972a, The structure and function of chromatin, in “Adv. in Human Genet.”, H. Harris and K. Hirschhorn, ed., 3: 237–431.

    Google Scholar 

  • Comings, D. E., 1972b, Methylation of euchromatic and heterochromatic DNA, Exp. Cell Res., 74: 383–390.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., 1975, Mechanisms of chromosome banding. IV. Optical properties of the Giemsa dyes, Chromosoma, 50: 89–110.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., 1978, Mechanisms of chromosome banding and implications for chromosome structure, Ann. Rev. Genet., 12: 25–46.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Okada, T. A., 1970, Whole-mount electron microscopy of the centromere region of metacentric and telocentric mammalian chromosomes, Cytogenetics, 9: 436–449.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Okada, T. A., 1971, Fine structure of kinetochore in Indian muntjac, Exp. Cell Res., 67: 97–110.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Okada, T.A., 1972, Electron microscopy of chromosomes, in: “Perspectives in Cytogenetics. The Next Decade,” S. W. Wright, B. F. Crandall, and L. Boyer, ed., Charles C. Thomas, Springfield, pp. 223–250.

    Google Scholar 

  • Comings, D. E., and Mattoccia, E., 1972, DNA of mammalian and avian heterochromatin, Exp. Cell Res., 71: 113–131.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Avelino, E., 1974, Mechanisms of chromosome banding. II. Evidence that histones are not involved, Exp. Cell Res., 86: 202–206.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., & Avelino, E., 1975, Mechanisms of chromosome banding. VII. Interaction of methylene blue with DNA and chromatin, Chromosoma, 51: 365–379.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Harris, D. C., 1975, Nuclear proteins. I. Electrophoretic comparison of mouse nucleoli, heterochromatin, euchromatin and contractile proteins, Exp. Cell Res., 96: 161–179.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Okada, T. A., 1975, Mechanisms of chromosome banding VI. Whole mount electron microscopy of banded metaphase chromosomes and comparison to pachytene chromosomes, Exp. Cell Res., 93: 267–274.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Drets, M. E., 1976, Mechanisms of chromosome banding. IX. Are variations in DNA base composition adequate to account for Quinacrine, Hoechst 33258 and Daunomycin banding? Chromosoma, 56: 199–211.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., and Wyandt, H. E., 1976, Reverse banding of Japanese quail microchromosomes, Exp. Cell Res., 99: 183–185.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., Avelino, E., Okada, T. A., and Wyandt, H. E., 1973, The mechanism of C- and G-banding of chromosomes, Exp. Cell Res., 77: 469–493.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., Kovacs, B. W., Avelino, E., and Harris, D. C., 1975, Mechanisms of chromosome banding. V. Quinacrine banding, Chromosoma, 50: 111–145.

    PubMed  CAS  Google Scholar 

  • Comings, D. E., Harris, D. C., Okada, T. A., and Holmquist, G., 1977, Nuclear proteins. IV. Deficiency of non-histone proteins in condensed chromatin of Drosophila virilis and mouse, Exp. Cell Res., 105: 349–365.

    PubMed  CAS  Google Scholar 

  • Cook, P. R., and Brazell, I. A., 1978, Spectrofluorometric measurement of the binding of ethidium to superhelical DNA from cell nuclei, Eur. J. Biochem., 84: 465–477.

    PubMed  CAS  Google Scholar 

  • Corneo, G., Ginelli, E., and Polli, E., 1970, Repeated sequences in human DNA, J. Mol. Biol., 48: 319–327.

    PubMed  CAS  Google Scholar 

  • Curtis, D., and Horobin, R. W., 1982, Chromosome banding: specification of structural features of dyes giving rise to G-banding, Histochem. J., 14: 911–928.

    PubMed  CAS  Google Scholar 

  • Daskal, Y., Mace, M. L., Wray, W., and Busch, H., 1976, Use of direct current sputtering for improved visualization of chromosome topology by scanning electron microscopy, Exp. Cell Res., 100: 204–212.

    PubMed  CAS  Google Scholar 

  • de la Chapelle, A., Schroder, J., and Selander, R.-K., 1971, Repetitious DNA in mammalian chromosomes, Hereditas, 69: 149–153.

    PubMed  Google Scholar 

  • Dev, V. G., Warburton, D., Miller, O. J., Miller, D. A., Erlanger, B. F., and Beiser, S. M., 1972, Consistent pattern of binding of anti-adenosine antibodies to human metaphase chromosomes, Exp. Cell Res., 74: 288–293.

    PubMed  CAS  Google Scholar 

  • DiNardo, S., Voelkel, K., and Sternglanz, R., 1984, DNA topoisomerase II mutant of Saccharomyces cerevisiae: Topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication, Proc. Natl. Acad. Sci. USA, 81: 2616–2620.

    PubMed  CAS  Google Scholar 

  • DuPraw, E. J., 1965, Macromolecular organization of nuclei and chromosomes: A folded fibre model based on whole-mount electron microscopy, Nature, 206: 338–343.

    PubMed  CAS  Google Scholar 

  • DuPraw, E. J., 1966, Evidence for a ‘folded-fibre’ organization in human chromosomes, Nature, 209: 577–581.

    PubMed  CAS  Google Scholar 

  • DuPraw, E. J., 1970, “DNA and Chromosomes”, Holt, Rinehart and Winston, Inc., New York.

    Google Scholar 

  • Dutrillaux, B., and Lejeune, J., 1971, Sur une nouvelle technique d’analyse du caryotype humain, C.R. Acad. Sci., (Paris), 272 (D): 2638–2640.

    CAS  Google Scholar 

  • Dutrillaux, B., and Lejeune, J., 1975, New techniques in the study of human chromosomes: Methods and applications, in “Adv. Human Genet.”, H. Harris and K. Hirschhorn, ed., 5: 119–156.

    Google Scholar 

  • Dutrillaux, B., Rethore, M.-O., and Lejeune, J., 1975, Comparaison du caryotype de l’orang-outang (Pongo pygmaeus) a celui de l’homme, du chimpanze et du gorille, Ann. Genet., 18: 153–161.

    PubMed  CAS  Google Scholar 

  • Earnshaw, W. C., and Laemmli, U. K., 1983, Architecture of metaphase chromosomes and chromosome scaffolds, J. Cell. Biol., 96: 84–93.

    PubMed  CAS  Google Scholar 

  • Earnshaw, W. C., and Laemmli, U. K., 1984, Silver staining the chromosome scaffold, Chromosoma, 89: 186–192.

    PubMed  CAS  Google Scholar 

  • Earnshaw, W. C., and Heck, M. M. S., 1985, Localization of topoisomerase II in mitotic chromosomes, J. Cell Biol., 100: 1716–1725.

    PubMed  CAS  Google Scholar 

  • Earnshaw, W. C., and Rothfield, N., 1985, Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma, Chromosoma, 91: 313–321.

    PubMed  CAS  Google Scholar 

  • Earnshaw, W. C., Halligan, N., Cooke, C., and Rothfield, N., 1984, The kinetochore is part of the metaphase chromosome scaffold, J. Cell Biol., 98: 352–357.

    PubMed  CAS  Google Scholar 

  • Earnshaw, W. C., Halligan, B., Cooke, C. A., Heck, M. M. S., and Liu, L. F., 1985, Topoisomerase II is a structural component of mitotic chromosome scaffolds, J. Cell Biol., 100: 1706–1715.

    PubMed  CAS  Google Scholar 

  • Ellison, J. R., and Barr, H. J., 1972, Quinacrine fluorescence of specific chromosome regions. Late replication and high A:T content in Samoaia leonensis, Chromosoma, 36: 375–390.

    PubMed  CAS  Google Scholar 

  • Epplen, J. T., Siebers, J.-W., and Vogel, W., 1975, DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique, Cytogenet. Cell Genet., 15, 177–185.

    PubMed  CAS  Google Scholar 

  • Felsenfeld, G., 1978, Chromatin, Nature, 271: 115–122.

    PubMed  CAS  Google Scholar 

  • Ganner, E., and Evans, H. J., 1971, The relationship between patterns of DNA replication and of Quinacrine fluorescence in the human chromosome complement, Chromosoma, 35: 326–341.

    PubMed  CAS  Google Scholar 

  • Gasser, S. M., Laroche, T., Falquet, J., Boy de la Tour, E., and Laemmli, U. K., 1986, Metaphase chromosome structure. Involvement of topoisomerase II, J. Mol. Biol., 188: 613–629.

    CAS  Google Scholar 

  • Gazit, B., Cedar, H., Lerer, I., and Voss, R., 1982, Active genes are sensitive to deoxyribonuclease I during metaphase, Science, 217: 648–650.

    PubMed  CAS  Google Scholar 

  • Goldman, M. A., Holmquist, G. P., Gray, M. C., Caston, L. A., and Nag, A., 1984, Replication timing of genes and middle repetitive sequences, Science, 224: 686–692.

    PubMed  CAS  Google Scholar 

  • Golomb, H. M., and Bahr, G. F., 1974, Correlation of the fluorescent banding pattern and ultrastructure of a human chromosome, Exp. Cell Res., 84: 121–126.

    PubMed  CAS  Google Scholar 

  • Goodpasture, C., and Bloom, S. E., 1975, Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining, Chromosoma, 53: 37–50.

    PubMed  CAS  Google Scholar 

  • Gormley, I. P., and Ross, A., 1972, Surface topography of human chromosomes examined at each stage during ASG banding procedure, Exp. Cell Res., 74: 585–587.

    Google Scholar 

  • Gormley, I. P., and Ross, A., 1976, Studies on the relationship of a collapsed chromosomal morphology to the production of Q- and G-bands, Exp. Cell Res., 98: 152–158.

    PubMed  CAS  Google Scholar 

  • Gottesfeld, J, M., 1977, Methods for fractionation of chromatin into transcriptionally active and inactive segments, in: “Methods in Cell Biol.”, D. M. Prescott, ed., XVI. Chromatin and Chromosomal Protein Research I, G. Stein, J. Stein, and L. J. Kleinsmith, ed., pp. 421–436, Academic Press, New York.

    Google Scholar 

  • Gottesfeld, J. M., Bonner, J., Radda, G. K., and Walker, I. O., 1974, Biophysical studies on the mechanism of Quinacrine staining of chromosomes, Biochem., 13: 2937–2945.

    CAS  Google Scholar 

  • Greilhuber, J., 1977, Why plant chromosomes do not show G-bands, Theor. Appl. Genet., 50: 121–124.

    Google Scholar 

  • Hadlaczky, G., Sumner, A. T., and Ross, A., 1981a, Protein-depleted chromosomes. I. Structure of isolated protein-depleted chromosomes, Chromosoma, 81: 537–555.

    PubMed  CAS  Google Scholar 

  • Hadlaczky, G., Sumner, A. T., and Ross, A., 1981b, Protein-depleted chromosomes. II. Experiments concerning the reality of chromosome scaffolds, Chromosoma, 81: 557–567.

    PubMed  CAS  Google Scholar 

  • Hadlaczky, G., Praznovszky, T., and Bisztray, G., 1982, Structure of isolated protein-depleted chromosomes of plants, Chromosoma, 86: 643–659.

    CAS  Google Scholar 

  • Hancock, J. M., and Sumner, A. T., 1982, The role of proteins in the production of different types of chromosome bands, Cytobios, 35: 37–46.

    PubMed  CAS  Google Scholar 

  • Hand, R., 1975, Regulation of DNA replication on subchromosomal units of mammalian cells, J. Cell Biol., 64: 89–97.

    PubMed  CAS  Google Scholar 

  • Hanks, S. K., Gollin, S. M., Rao, P. N., Wray, W., and Hittelman, W. N., 1983, Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes, Chromosoma, 88: 333–342.

    PubMed  CAS  Google Scholar 

  • Harrison, C. J., Britch, M., Allen, T. D., and Harris, R., 1981, Scanning electron microscopy of the G-banded human karyotype, Exp. Cell Res., 134: 141–153.

    PubMed  CAS  Google Scholar 

  • Harrison, C. J., Allen, T. D., Britch, M., and Harris, R., 1982, High-resolution scanning electron microscopy of human metaphase chromosomes, J. Cell Sci., 56: 409–422.

    PubMed  CAS  Google Scholar 

  • Harrison, C. J., Allen, T. D., and Harris, R., 1983, Scanning electron microscopy of variations in human metaphase chromosome structure revealed by Giemsa banding, Cytogenet. Cell Genet., 35: 21–27.

    PubMed  CAS  Google Scholar 

  • Holliday, R., 1964, A mechanism for gene conversion in fungi, Genet. Res., 5: 282–304.

    Google Scholar 

  • Holmquist, G., 1979, The mechanism of C-banding: Depurination and ß-elimination, Chromosoma, 72: 203–224.

    PubMed  CAS  Google Scholar 

  • Holmquist, G. P., and Comings, D. E., 1976, Histones and G banding of chromosomes, Science, 193: 599–602.

    PubMed  CAS  Google Scholar 

  • Holmquist, G., Gray, M., Porter, T., and Jordan, J., 1982, Characterization of Giemsa dark and light band DNA, Cell, 31: 121–129.

    PubMed  CAS  Google Scholar 

  • Horz, W., and Altenburger, W., 1981, Nucleotide sequence of mouse satellite DNA, Nucleic Acids Res., 9: 683–696.

    PubMed  CAS  Google Scholar 

  • Howell, W. M., and Hsu, T. C., 1979, Chromosome core structure revealed by silver staining, Chromosoma, 73: 61–66.

    PubMed  CAS  Google Scholar 

  • Howell, W.M., Denton, T. E., and Diamond, J. R., 1975, Differential staining of the satellite regions of human acrocentric chromosomes, Experientia, 31: 260–262.

    PubMed  CAS  Google Scholar 

  • Hsu, T. C., 1974, Longitudinal differentiation of chromosomes, Ann. Rev. Genet., 7: 153–176.

    CAS  Google Scholar 

  • Hsu, T. C., and Arrighi, F. E., 1971, Distribution of constitutive heterochromatin in mammalian chromosomes, Chromosoma, 34: 243–253.

    PubMed  CAS  Google Scholar 

  • Hsu, T. C., Pathak, S., and Shafer, D. A., 1973, Induction of chromosome crossbanding by treating cells with chemical agents before fixation, Exp, Cell Res., 79: 484–487.

    CAS  Google Scholar 

  • Huberman, J. A., and Riggs, A. D., 1968, On the mechanism of DNA replication in mammalian chromosomes, J. Mol. Biol., 32: 327–341.

    PubMed  CAS  Google Scholar 

  • Igo-Kemenes, T., and Zachau, H. G., 1978, Domains in chromatin structure, Cold Spring Harbor Symp. Quant. Biol., 42: 109–118.

    PubMed  CAS  Google Scholar 

  • Igo-Kemenes, T., Horz., W., and Zachau, H. G., 1982, Chromatin, Ann Rev. Biochem., 51: 89–121.

    CAS  Google Scholar 

  • Jhanwar, S. C., Burns, J. P., Alonso, M. L., Hew, W., and Chaganti, R. S. K., 1982, Mid-pachytene chromomere maps of human autosomes, Cytogenet. Cell Genet., 33: 240–248.

    PubMed  CAS  Google Scholar 

  • Jones, K. W., and Corneo, G., 1971, Location of satellite and homogeneous DNA sequences on human chromosomes, Nature New Biol., 233: 268–271.

    PubMed  CAS  Google Scholar 

  • Kaelbling, M., Miller, D. A., and Miller, O. J., 1984, Restriction enzyme banding of mouse metaphase chromosomes, Chromosoma, 90: 128–132.

    PubMed  CAS  Google Scholar 

  • Kaiserman, M. Z., and Burkholder, G. D., 1980, Silver stained core-like structures in Chinese hamster metaphase chromosomes, Can. J. Genet. Cytol., 22: 627–632.

    PubMed  CAS  Google Scholar 

  • Kato, H., and Moriwaki, K., 1972, Factors involved in the production of banded structures in mammalian chromosomes, Chromosoma, 38: 105–120.

    PubMed  CAS  Google Scholar 

  • Kavenoff, R., and Zimm, B. H., 1973, Chromosome-sized DNA molecules from Drosophila, Chromosoma, 41: 1–27.

    PubMed  CAS  Google Scholar 

  • Kerem, B-S., Goitein, R., Diamond, G., Cedar, H., and Marcus, M., 1984, Mapping of DNAase I sensitive regions on mitotic chromosomes, Cell, 38: 493–499.

    PubMed  CAS  Google Scholar 

  • Kingwell, B., and Rattner, J.B., 1986, Sister chromatids are associated together at discrete points in metaphase chromosomes, J. Cell Biol., 103: 492a.

    Google Scholar 

  • Kitchin, R. M., and Loudenslager, E. J., 1976, An in vivo Giemsa chromosome banding technique, Stain Technol., 50: 371–374.

    Google Scholar 

  • Korenberg, J. R., and Engels, W. R., 1978, Base ratio, DNA content, and quinacrine-brightness of human chromosomes, Proc. Natl. Acad. Sci. USA, 75: 3382–3386.

    PubMed  CAS  Google Scholar 

  • Korenberg, J. R., Therman, E., and Denniston, C., 1978, Hot spots and functional organization of human chromosomes, Human Genet., 43: 13–22.

    CAS  Google Scholar 

  • Labhart, P., Koller, T., and Wunderli, H, 1982, Involvement of higher order chromatin structures in metaphase chromosome organization, Cell, 30: 115–121.

    PubMed  CAS  Google Scholar 

  • Laemmli, U. K., Cheng, S. M., Adolph, K. W., Paulson, J. R., Brown, J. A., and Baumbach, W. R., 1978, Metaphase chromosome structure: The role of nonhistone proteins, Cold Spring Harbor Symp. Quant. Biol., 42: 351–360.

    PubMed  CAS  Google Scholar 

  • Laird, C. D., 1971, Chromatid structure: Relationship between DNA content and nucleotide sequence diversity, Chromosoma, 32: 378–406.

    PubMed  CAS  Google Scholar 

  • Latt, S. A., 1973, Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes, Proc. Natl. Acad. Sci., USA, 70: 3395–3399.

    PubMed  CAS  Google Scholar 

  • Latt, S. A., 1976, Optical studies of metaphase chromosome organization, Ann. Rev. Biophys. & Bioeng., 5: 1–37.

    CAS  Google Scholar 

  • Laughlin, T. J., Wilkinson-Singley, E., Olins, D. E., and Olins, A. L., 1982, Stereo electron microscope studies of mitotic chromosomes from Chinese hamster ovary cells, Eur. J. Cell Biol., 27: 170–176.

    PubMed  CAS  Google Scholar 

  • Lebkowski, J., and Laemmli, U. K., 1982, Evidence for two levels of DNA folding in histone-depleted HeLa interphase nuclei, J. Mol. Biol., 156: 309–324.

    PubMed  CAS  Google Scholar 

  • Lepault, J., Bram, S., Escaig, J., and Wray, W., 1980, Chromatin freeze fracture electron microscopy: A comparative study of core particles, chromatin, metaphase chromosomes, and nuclei, Nucleic Acids Res., 8: 265–278.

    PubMed  CAS  Google Scholar 

  • Lewis, C. D., and Laemmli, U. K., 1982, Higher order metaphase chromosome structure: Evidence for metalloprotein interactions, Cell, 29: 171–181.

    PubMed  CAS  Google Scholar 

  • Lica, L., and Hamkalo, B., 1983, Preparation of centromeric heterochromatin by restriction endonuclease digestion of mouse L929 cells, Chromosoma, 88: 42–49.

    PubMed  CAS  Google Scholar 

  • Lubs, H. A., McKenzie, W. H., and Merrick, S., 1973, Comparative methodology and mechanisms of banding, in: “Chromosome Identification—Techniques and Applications in Biology and Medicine,” T. Caspersson and L. Zech, ed., pp. 315–322, Academic Press, New York.

    Google Scholar 

  • Magaud, J.-P., Rimokh, R., Brochier, J., Lafage, M., and Germain, D., 1985, Chromosomal R-banding with a monoclonal antidouble-stranded DNA antibody, Human Genet., 69: 238–242.

    CAS  Google Scholar 

  • Marsden, M. P. F., and Laemmli, U. K., 1979, Metaphase chromosome structure: Evidence for a radial loop model, Cell, 17: 849–858.

    PubMed  CAS  Google Scholar 

  • Matsui, S., Chai, L., Tsui, S., and Sandberg, A. A., 1985, Chromosome structure in the absence of non-histone chromosomal proteins (NHCP), J. Cell Biol., 101: 75a.

    Google Scholar 

  • Matsukuma, S., and Utakoji, T., 1976, Uneven extraction of protein in Chinese hamster chromosomes during G-staining procedures, Exp. Cell Res., 97: 297–303.

    PubMed  CAS  Google Scholar 

  • Matsukuma, S., and Utakoji, T., 1977, Non-histone protein associated with centromeric heterochromatin in the mouse chromosome, Exp. Cell Res., 105: 217–222.

    PubMed  CAS  Google Scholar 

  • Mattern, M. R., Paone, R. F., and Day III, R. S., 1982, Eukaryotic DNA repair is blocked at different steps by inhibition of DNA topoisomerases and of DNA polymerase α and ß, Biochim. Biophys. Acta, 697: 6–13.

    PubMed  CAS  Google Scholar 

  • Mayfield, J. E., and McKenna, J. F., 1978, A-T rich sequences in vertebrate DNA. A possible explanation of Q-banding in metaphase chromosomes, Chromosoma, 67: 157–163.

    PubMed  CAS  Google Scholar 

  • Mazrimas, J. A., Balhorn, R., and Hatch, F. T., 1979, Separation of satellite DNA chromatin and main band DNA chromatin from mouse brain, Nucleic Acids Res., 7: 935–946.

    PubMed  CAS  Google Scholar 

  • McGhee, J. D., and Felsenfeld, G., 1980, Nucleosome structure, Ann. Rev, Biochem., 49:1115–1156.

    CAS  Google Scholar 

  • McKay, R. D. G., 1973, The mechanism of G and C banding in mammalian metaphase chromosomes, Chromosoma, 44: 1–14.

    PubMed  CAS  Google Scholar 

  • Mezzanotte, R., Bianchi, U., Vanni, R., and Ferrucci, L., 1983, Chromatin organization and restriction endonuclease activity on human metaphase chromosomes, Cytogenet. Cell Genet., 36: 562–566.

    PubMed  CAS  Google Scholar 

  • Mezzanotte, R., Ferrucci, L., Vanni, R., and Sumner, A. T., 1985, Some factors affecting the action of restriction endonucleases on human metaphase chromosomes, Exp. Cell Res., 161: 247–253

    PubMed  CAS  Google Scholar 

  • Miller, D. A., Choi, Y.-C., and Miller, O. J., 1983, Chromosome localization of highly repetitive human DNA’s and amplified ribosomal DNA with restriction enzymes, Science, 219: 395–397.

    PubMed  CAS  Google Scholar 

  • Miller, D. A., Gosden, J. R., Hastie, N. D., and Evans, H. J., 1984, Mechanism of endonuclease banding of chromosomes, Exp. Cell Res., 155: 294–298.

    PubMed  CAS  Google Scholar 

  • Miller, O. J., Schnedl W., Allen, J., and Erlanger, B. F., 1974, 5-methylcytosine localised in mammalian constitutive heterochromatin, Nature, 251: 636–637.

    PubMed  CAS  Google Scholar 

  • Miller, D. A., Dev, V. G., Tantravahi, R., and Miller, O. J., 1976, Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells, Exp. Cell Res., 101: 235–243.

    PubMed  CAS  Google Scholar 

  • Mirkovitch, J., Mirault, M.-E., and Laemmli, U. K., 1984, Organization of the higher-order chromatin loop: Specific DNA attachment sites on nuclear scaffold, Cell, 39: 223–232.

    PubMed  CAS  Google Scholar 

  • Mirkovitch, J., Spierer, P., and Laemmli, U. K., 1986, Genes and loops in 320,000 base-pairs of the Drosophila melanogaster chromosome, J. Mol. Biol., 190: 255–258.

    PubMed  CAS  Google Scholar 

  • Moliter, H., Drahovsky, D., and Wacker, A., 1974, Structural integrity of chromatid DNA in mouse L cells, J. Mol. Biol., 86: 161–163.

    Google Scholar 

  • Musich, P. R., Brown, F. L., and Maio, J. J., 1977, Subunit structure of chromatin and the organization of eukaryotic highly repetitive DNA: Nucleosomal proteins associated with a highly repetitive mammalian DNA, Proc. Natl. Acad. Sci., USA, 74: 3297–3301.

    PubMed  CAS  Google Scholar 

  • Nasedkina, T. V., and Slesinger, S. I., 1982, The structure of partly decondensed metaphase chromosomes, Chromosoma, 86: 239–249.

    PubMed  CAS  Google Scholar 

  • Nash, W. G., and O’Brien, S. J., 1982, Conserved regions of homologous G-banded chromosomes between orders in mammalian evolution: Carnivores and primates, Proc. Natl. Acad. Sci. USA, 79: 6631–6635.

    PubMed  CAS  Google Scholar 

  • Nelson, W. G., Liu, L. F., and Coffey, D. S., 1986, Newly replicated DNA is associated with DNA topoisomerase II in cultured rat prostatic adenocarcinoma cells, Nature, 322: 187–189.

    PubMed  CAS  Google Scholar 

  • Ohnuki, Y., 1968, Structure of chromosomes. I. Morphological studies of the spiral structure of human somatic chromosomes, Chromosoma, 25: 402–428.

    PubMed  CAS  Google Scholar 

  • Okada, T. A., & Comings, D. E., 1974, Mechanisms of chromosome banding. III. Similarity between G-bands of mitotic chromosomes and chromomeres of meiotic chromosomes, Chromosoma, 48: 65–71.

    PubMed  CAS  Google Scholar 

  • Okada, T.A., and Comings, D. E., 1979, Higher order structure of chromosomes, Chromosoma, 72: 1–14.

    PubMed  CAS  Google Scholar 

  • Okada, T. A., and Comings, D. E., 1980, A search for protein cores in chromosomes: Is the scaffold an artifact? Am. J. Hum. Genet., 32: 814–832.

    PubMed  CAS  Google Scholar 

  • Pachmann, U., and Rigler, R., 1972, Quantum yield of acridines interacting with DNA of defined base sequence. A basis for the explanation of acridine bands in chromosomes, Exp. Cell Res., 72: 602–608.

    PubMed  CAS  Google Scholar 

  • Pardue, M. L., and Gall, J. G., 1970, Chromosomal localization of mouse satellite DNA, Science, 168: 1356–1358.

    PubMed  CAS  Google Scholar 

  • Pathak, S., and Arrighi, F. E., 1973, Loss of DNA following C-banding procedures, Cytogenet. Cell Genet., 12: 414–422.

    PubMed  CAS  Google Scholar 

  • Paulson, J. R., and Laemmli, U. K., 1977, The structure of histone-depleted metaphase chromosomes, Cell, 12: 817–828.

    PubMed  CAS  Google Scholar 

  • Pothier, L,, Gallagher, J. F., Wright, C. E., and Libby, P. R., 1975, Histones in fixed cytological preparations of Chinese hamster chromosomes demonstrated by immunofluorescence, Nature, 255: 350–352.

    PubMed  CAS  Google Scholar 

  • Rattner, J. B., and Hamkalo, B. A., 1978a, Higher order structure in metaphase chromosomes. I. The 250 A fiber, Chromosoma, 69: 363–372.

    PubMed  CAS  Google Scholar 

  • Rattner, J. B., and Hamkalo, B. A., 1978b, Higher order structure in metaphase chromosomes. II. The relationship between the 250 A° fiber, superbeads,and beads-on-a-string, Chromosoma, 69: 373–379.

    PubMed  CAS  Google Scholar 

  • Rattner, J. B., and Lin, C. C., 1985, Radial loops and helical coils coexist in metaphase chromosomes, Cell, 42: 291–296.

    PubMed  CAS  Google Scholar 

  • Rattner, J. B., Krystal, G., and Hamkalo, B. A., 1978, Selective digestion of mouse metaphase chromosomes, Chromosoma, 66: 259–268.

    PubMed  CAS  Google Scholar 

  • Retief, A. E., and Ruchel, R., 1977, Histones removed by fixation. Their role in the mechanism of chromosomal banding, Exp. Cell Res., 106: 233–237.

    PubMed  CAS  Google Scholar 

  • Roos, U.-P., 1973, Light and electron microscopy of rat kangaroo cells in mitosis. II. Kinetochore structure and function, Chromosoma, 41: 195–220.

    PubMed  CAS  Google Scholar 

  • Ross, A., and Gormley, I. P., 1973, Examination of surface topography of Giemsa-banded human chromosomes by light and electron microscopic techniques, Exp. Cell Res., 81: 79–86.

    PubMed  CAS  Google Scholar 

  • Rowley, J. D., and Bodmer, W. F., 1971, Relationship of centromeric heterochromatin to fluorescent banding patterns of metaphase chromosomes in the mouse, Nature, 231: 503–506.

    PubMed  CAS  Google Scholar 

  • Sahasrabuddhe, C. G., Pathak, S., and Hsu, T. C., 1978, Responses of mammalian metaphase chromosomes to endonuclease digestion, Chromosoma, 69: 331–338.

    PubMed  CAS  Google Scholar 

  • Sawyer, J. R., and Hozier, J. C., 1986, High resolution of mouse chromosomes: Banding conservation between man and mouse, Science, 232: 1632–1635.

    PubMed  CAS  Google Scholar 

  • Schweizer, D., 1976, Reverse fluorescent chromosome banding with chromomycin and DAPI, Chromosoma, 58: 307–324.

    PubMed  CAS  Google Scholar 

  • Sedat, J., and Manuelidis, L., 1978, A direct approach to the structure of eukaryotic chromosomes, Cold Spring Harbor Symp. Quant. Biol., 42: 331–350.

    PubMed  CAS  Google Scholar 

  • Sehested, J., 1974, A simple method for R-banding of human chromosomes, showing a pH-dependent connection between R and G bands, Humangenetik, 21: 55–58.

    PubMed  CAS  Google Scholar 

  • Sivak, A., and Wolman, S. R., 1974, Chromosomal proteins in fixed metaphase cells, Histochem., 42: 345–349.

    CAS  Google Scholar 

  • Southern, E. M., 1975, Long range periodicity in mouse satellite DNA, J. Mol. Biol., 94: 51–69.

    PubMed  CAS  Google Scholar 

  • Sperling, K., and Rao, P. N., 1974, The phenomenon of premature chromosome condensation: Its relevance to basic and applied research, Humangenetik, 23: 235–258.

    PubMed  CAS  Google Scholar 

  • Steck, T. R., and Drlica, K., 1984, Bacterial chromosome segregation: Evidence for DNA gyrase involvement in decatenation, Cell, 36: 1081–1088.

    PubMed  CAS  Google Scholar 

  • Stubblefield, E., and Wray, W., 1971, Architecture of the Chinese hamster metaphase chromosome, Chromosoma, 32: 262–294.

    PubMed  CAS  Google Scholar 

  • Sumner, A. T., 1972, A simple technique for demonstrating centromeric heterochromatin, Exp. Cell Res., 75: 304–306.

    PubMed  CAS  Google Scholar 

  • Sumner, A. T., 1974, Involvement of protein disulphides and sulphydryls in chromosome banding, Exp. Cell Res., 83: 438–442.

    PubMed  CAS  Google Scholar 

  • Sumner, A. T., 1980, Dye binding mechanisms in G-banding of chromosomes, J. Micros., 119: 397–406.

    CAS  Google Scholar 

  • Sumner, A. T., 1981, The distribution of quinacrine on chromosomes as determined by X-ray microanalysis. I. Q-bands on CHO chromosomes, Chromosoma, 82: 717–734.

    PubMed  CAS  Google Scholar 

  • Sumner, A. T., 1982, The nature and mechanisms of chromosome banding, Cancer Genet. & Cytogenet., 6: 59–87.

    CAS  Google Scholar 

  • Sumner, A. T., 1985, The distribution of quinacrine on chromosomes as determined by X-ray microanalysis. II. Comparison of heterochromatic and euchromatic regions of mouse chromosomes, Chromosoma, 91: 145–150.

    PubMed  CAS  Google Scholar 

  • Sumner, A. T., and Evans, H. J., 1973, Mechanisms involved in the banding of chromosomes with Quinacrine and Giemsa. II. The interaction of the dyes with the chromosomal components, Exp. Cell Res., 81: 223–236.

    PubMed  CAS  Google Scholar 

  • Sumner, A. T., Evans, H. J., and Buckland, R. A., 1971, New technique for distinguishing between human chromosomes, Nature New Biol., 232: 31–32.

    PubMed  CAS  Google Scholar 

  • Sumner, A. T., Evans, H. J., and Buckland, R. A., 1973, Mechanisms involved in the banding of chromosomes with Quinacrine and Giemsa. I. The effects of fixation in methanol-acetic acid, Exp. Cell Res., 81: 214–222.

    PubMed  CAS  Google Scholar 

  • Takayama, S., 1976, Configurational changes in chromatids from helical to banded structures, Chromosoma, 56: 47–54.

    PubMed  CAS  Google Scholar 

  • Taniguchi, T., and Takayama, S., 1986, High-order structure of metaphase chromosomes: Evidence for a multiple coiling model, Chromosoma, 93: 511–514.

    PubMed  CAS  Google Scholar 

  • Taylor, J. H., 1958, The duplication of chromosomes, Sci. Amer., 198: 36–42.

    PubMed  CAS  Google Scholar 

  • Tobia, A., Schildkraut, C. L., and Maio, J. J., 1970, Deoxyribonucleic acid replication in synchronized cultured mammalian cells. I. Time of synthesis of molecules of different average guanine + cytosine content, J. Mol. Biol., 54: 499–515.

    PubMed  CAS  Google Scholar 

  • Uemura, T., and Yanagida, M., 1984, Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization, EMBO J., 3: 1737–1744.

    PubMed  CAS  Google Scholar 

  • Utsumi, K. R., 1982, Scanning electron microscopy of Giemsa-stained chromosomes and surface-spread chromosomes, Chromosoma, 86: 683–702.

    PubMed  CAS  Google Scholar 

  • Valdivia, M. M., and Brinkley, B. R., 1985, Fractionation and initial characterization of the kinetochore from mammalian metaphase chromosomes, J. Cell Biol., 101: 1124–1134.

    PubMed  CAS  Google Scholar 

  • van de Sande, J. H., Lin, C. C., and Jorgenson, K. F., 1977, Reverse banding on chromosomes produced by a guanosine-cytosine specific DNA binding antibiotic: olivomycin, Science, 195: 400–402.

    PubMed  Google Scholar 

  • van Duijn, P., van Prooijen-Knegt, A. C., and van der Ploeg, M., 1985, The involvement of nucleosomes in Giemsa staining of chromosomes. A new hypothesis on the banding mechanism, Histochem., 82: 363–376.

    Google Scholar 

  • Verma, R. S., and Lubs, H. A., 1975, A simple R banding technic, Am. J. Hum. Genet., 27: 110–117.

    PubMed  CAS  Google Scholar 

  • Vogel, W., Faust, J., Schmid, M., and Siebers, J.-W., 1974, On the relevance of non-histone proteins to the production of Giemsa banding patterns on chromosomes, Humangenetik, 21: 227–236.

    PubMed  CAS  Google Scholar 

  • Vogelstein, B., Pardoll, D. M., and Coffey, D. S., 1980, Supercoiled loops and eukaryotic DNA replication, Cell, 22: 79–85.

    PubMed  CAS  Google Scholar 

  • Wang, H. C., 1987, personal communication.

    Google Scholar 

  • Wang, H. C., and Fedoroff, S., 1972, Banding in human chromosomes treated with trypsin, Nature New Biol., 235: 52–53.

    PubMed  CAS  Google Scholar 

  • Wang, J. C., 1985, DNA topoisomerases, Ann. Rev. Biochem., 54: 665–697.

    PubMed  CAS  Google Scholar 

  • Weintraub, H., 1985, Assembly and propagation of repressed and derepressed chromosomal states, Cell, 42: 705–711.

    PubMed  CAS  Google Scholar 

  • Weisblum, B., and deHaseth, P. L., 1972, Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylate-rich regions in DNA, Proc. Natl. Acad. Sci. USA, 69: 629–632.

    PubMed  CAS  Google Scholar 

  • Weisblum, B., and de Haseth, P. L., 1973, Nucleotide specificity of the quinacrine staining reaction for chromosomes, Chromosomes Today, 4: 35–51.

    CAS  Google Scholar 

  • Wunderli, H., Westphal, M., Armbruster, B., and Labhart, P, 1983, Comparative studies on the structural organization of membrane-depleted nuclei and metaphase chromosomes, Chromosoma, 88: 241–248.

    PubMed  CAS  Google Scholar 

  • Yunis, J. J., 1981, Mid-prophase human chromosomes. The attainment of 2000 bands, Human Genet., 56: 293–298.

    CAS  Google Scholar 

  • Yunis, J. J., and Sanchez, O., 1973, G-banding and chromosome structure, Chromosoma, 44: 15–23.

    PubMed  CAS  Google Scholar 

  • Yunis, J. J., and Prakash, O., 1982, The origin of man: A chromosomal pictorial legacy, Science, 215: 1525–1530.

    PubMed  CAS  Google Scholar 

  • Yunis, J. J., Kuo, M. T., and Saunders, G. F., 1977, Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes, Chromosoma, 61: 335–344.

    PubMed  CAS  Google Scholar 

  • Yunis, J. J., Sawyer, J. R., and Ball, D. W., 1978, The characterization of high-resolution G-banded chromosomes of man, Chromosoma, 67: 293–307.

    PubMed  CAS  Google Scholar 

  • Zhang, X. Y., and Horz, W., 1982, Analysis of highly purified satellite DNA containing chromatin from the mouse, Nucleic Acids Res., 10: 1481–1494.

    PubMed  CAS  Google Scholar 

  • Zheng, H.-Z., and Burkholder, G. D., 1982, Differential silver staining of chromatin in metaphase chromosomes, Exp. Cell Res., 141: 117–125.

    PubMed  CAS  Google Scholar 

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Burkholder, G.D. (1988). The Analysis of Chromosome Organization by Experimental Manipulation. In: Gustafson, J.P., Appels, R. (eds) Chromosome Structure and Function. Stadler Genetics Symposia Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1037-2_1

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