Effect of Fish Oil Feeding on Rat Heart Lipids : Composition and Ultramicroscopic Study

  • Gilles Nalbone
  • Jeannie Léonardi
  • Elise Termine
  • Henri Portugal
  • Paulette Lechêne
  • Huguette Lafont
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 150)


Long chain polyeneoic fatty acids (PUFA) have received large attention because of their beneficial effect on the cardiovascular system1. Among these fatty acids those of the n-3 family, largely represented in fish oils, were demonstrated to be more efficient in lowering plasma triglyceride and cholesterol levels than were the PUFA originating from vegetable oils2-7. Although fish oils may bring interesting approaches to prevent or treat atherosclerosis, they may however, adversely affect some vital functions like spleen prostaglandin production”, or give accumulation of degradative products of peroxydized lipids in tissues9-11. Few investigations have been devoted to the effect of fish oils feeding on heart lipid metabolism12, 13. In our comparative study rats were fed a salmon oil enriched diet for two months others a corn oil-enriched diet and the last group a low fat diet. Hearts lipids were analyzed and possible high fat-induced lipid accumulation in tissue was investigated by ultramicroscopic examination of ventricles.


Unsaturation Index Heart Lipid Lower Plasma Triglyceride Peroxydized Lipid Ultramicroscopic Examination 
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  1. 1.
    S.H. Goodnight Jr, W.S. Harris, W.E. Connor and D.R. Illingworth, Polyunsaturated fatty acids, hyperlipidemia and thrombosis, arteriosclerosis. 2, 87–113 (1982).PubMedCrossRefGoogle Scholar
  2. 2.
    J. Dyerberg, H.O. Bang, E. Stofferson, S. Moncada and J.R. Vane, Eicosapentaenoic acid and prevention of thrombosis and atherosclerosis. Lancet 2:117–119 (1978).PubMedCrossRefGoogle Scholar
  3. 3.
    J.G. Hauge and R. Nicolaysen, The serum cholesterol depressive effect of linoleic, linolenic acids and of cod liver oil in experimental hypercholesterolemia rats. Acta Physiol. Scand. 45:26–30 (1959).Google Scholar
  4. 4.
    H. Dam, G. Kristensen, G.K. Nielsen and E. Sondergaard, Influence of dietary cholesterol cold liver oil and lindseed oil on cholesterol and polyenoic fatty acids in tissues from fasted and non-fasted chicks. Acta Physiol. Scand. 45:31-42 (1959).Google Scholar
  5. 5.
    W.S. Harris, W.E. Connor and M.P. Mc Murry, The comparative reductions of the plasma lipids and lipoproteins by dietary polyunsaturated fats: salmon oil versus vegetable oils. Metabolism 32:179-184 (1983).Google Scholar
  6. 6.
    D. Kromhout, E.B. Bosschieter and C.L. Coulander, The inverse relation between fish comsuption and 20-year mortality from coronary heart disease. N. Eng. J. Med. 312:1205-1209 (1985).Google Scholar
  7. 7.
    G. Durand, G. Pascal, P. Legrand and H. Goune.lle de Pontanel, Comparison of the effects of vegetable oils and fish oils on serum cholesterol level in the rat: relationships between the fatty acid composition of dietary lipids, that of serum lipids, and the serum cholesterol level. Med. et Nutr. 21:391-406 (1985).Google Scholar
  8. 8.
    B.R. Lokesh, H.L. Hsieh and J.E. Kinsella, Alteration in the lipids and prostaglandins in mouse spleen following the ingestion of menhaden oil. Ann. Nutr. Metab. 30:357-364 (1986).Google Scholar
  9. 9.
    C.T. Hammer and E.D. Wills, The role of lipid components of the diet in the regulation of the fatty acid composition of the rat liver endoplasmic reticulum and lipid peroxydation. Biochem. J. 174:585-593 (1978).Google Scholar
  10. 10.
    A. Ruiter, A.W. Jongbloed, C.M. van Gent, L.H.J.C Danse and S.H.M. Metz, The influence of dietary mackerel oil on the condition of organs and on blood lipid composition in the young pig. Am. J. Clin. Nutr. 31:2159-2166 (1978).Google Scholar
  11. 11.
    J. Opsvedt, H. Svaar, P. Hansen, J. Pettersen, F.T. Langmark, S.M. Barlow and I.F. Duthie, Comparison of lipid status in the hearts of piglets and rats on short tem feeding of marine oils and rapeseed oils. Lipids 14:356-371 (1979).Google Scholar
  12. 12.
    J.E. Swanson and J.E. Kinsella, Dietary n-3 polyunsaturated fatty acids: modification of rat cardiac lipids and fatty acid composition. J. Nutr. 116:514-523 (1986).Google Scholar
  13. 13.
    J.S. Charnock, M.Y. Abeywardena and R.L. Mc Lennan, Comparative changes in the fatty acid composition of rat cardiac phospholipids after long-term feeding of sunflower seed oil-or tuna fish oilsupplemented diets. Ann. Nutr. metab. 30:393-406 (1986).Google Scholar
  14. 14.
    J. Leonardi, E. Termine, F. Morand, R. Lafont, H. Portugal, H. Lafont and G. Nalbone, Effect of dietary lipids on the lipid composition and phospholipid deacylating enzyme activities of rat heart. Lipids 22:517-522 (1987).Google Scholar
  15. 15.
    E. Termine, J. Leonardi, H. Lafont and G. Nalbone, Intracellular phospholipase activity in rat heart: comparison between endogenous and exogenous substrates. Biochimie 69:245-248 (1987).Google Scholar
  16. 16.
    G. Cecchi, S. Biasini and J. Castano, Methanolyse rapide des huiles en solvant. Rev. Fr. Corps Gras 32:163-164 (1985).Google Scholar
  17. 17.
    A.P. De Leenheer, V.O.R.C. De Bevere, M.G.M. De Ruyter and A.E. Clayes, Simultaneous determination of retinol and alpha-tocopherol in human serum by high-performance liquid chromatography. J. Chromatogr. 162:408–413 (1979).PubMedCrossRefGoogle Scholar
  18. 18.
    S.N. Meydani, A.C. Shapiro, M. Meydani, J.B. Macauley and J.B. Blumberg, Effect of age and dietary fat (fish, corn and coconut oils) on tocopherol status of C57BL/6 Nia mice. Lipids 22:35–350 (1987).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Gilles Nalbone
    • 1
  • Jeannie Léonardi
    • 1
  • Elise Termine
    • 1
  • Henri Portugal
    • 1
  • Paulette Lechêne
    • 1
  • Huguette Lafont
    • 1
  1. 1.INSERM Unité 130MarseilleFrance

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