The Role of a New Glucosylceramidase Activator Protein in the Binding of the Enzyme to its Natural Substrate

  • Anna Maria Vaccaro
  • Michele Muscillo
  • Rosa Salvioli
  • Massimo Tatti
  • Elisabetta Gallozzi
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 150)

Abstract

Glucosylceramidase (EC 3.2.1.45) is a membrane-bound, lysosomal enzyme, which hydrolyzes glucosylceramide into glucose and ceramide (1). As for several other glycosphingolipid hydrolses, also for glucosylceramidase a special activator protein capable of stimulating its activity has been described in the past (2–5). It is mainly present in human spleen and, for this reason, has been called “Gaucher factor”. It is a water soluble protein with a molecular weight of about 13,000 Da (5).

Keywords

Hydrolysis Sucrose Filtration Albumin Citrate 

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References

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Anna Maria Vaccaro
    • 1
  • Michele Muscillo
    • 1
  • Rosa Salvioli
    • 1
  • Massimo Tatti
    • 1
  • Elisabetta Gallozzi
    • 1
  1. 1.Department of Metabolism and Pathological BiochemistryIstituto Superiore di SanitàRomeItaly

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