Abstract
During the past few years advances have been made in understanding the structure and the function of the photosynthetic unit (PSU) of green bacteria (Olson, 1980; Fetisova and Borisov, 1960; Betti et al., 1962; Wechsler et al., 1964; flmesz, 1965; Zuber, 1965; Amesz and Vasmel, 1986; van Dorssen et al., 1986a,b; Fetisova et al., 1966; Fetisova et al., 1967). In these studies the progress of preparative biochemistry proved to be decisive, since a major reason for the paucity of data on the primary events in the photosynthesis of green bacteria was undoubtedly the large antenna. The available data provide information about the structure and function of various isolated subcellular pigmented fractions from green bacteriaj whereas hardly anything is known about the functioning of the PSU in intact cells under physiological conditions. Because of their large antenna size (largest of alt the known PSUs) and the high quantum yield of primary charge separation in the reaction center (RC) (Fetisova and Borisov, 1960) this family of organisms is most promising (Fetisova and Fok, 1964) in the search for the fundamental principles of the PSU structural organization which provide large and highly efficient PSUs in vivo. This work presents the results of the first direct measurements of picosecond excitation energy transfer rates within a light-harvesting antenna in living cells of the green bacterium Chlorobium limicola as welt as the results of the investigation of some structural aspects of this energy transfer.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Amesz, J., 1965, Photosynthesis: structure of the membrane and membrane proteins, Progress in Botany, 47:67.
Amesz, J., and Vasmet, H., 1986, Fluorescence properties of photosynthetic bacteria, in: “Light Emission by Plants and Bacteria,” Govindjee, J. Amesz and D.C. Fork, eds., Academic Press, New York.
Betti, J.A., Blankenship, R.E., Natarajanf L.V., Dickinson, L.C., and Fuller, R.C., 1982, Antenna organization and evidence for the function of a new antenna pigment species in the green photosynthetic bacterium Chloroftexus aurantiacus, Biochim. Biophys. Acta, 680:194.
Bolt, J.D., and Sauer, K., 1981, Fluorescence properties of the tight-harvesting biacteriochlorophylL protein from Rhodopseudomonas sphaeroides R-26, Biochim. Biophys. Acta 637:342.
Borisov, A. Yu., Fetisova, Z.G., and Godik, V.I., 1977, Energy transfer in photoactive complexes obtained from the green bacterium Chlorobium limicola, Biochim. Biophys. Acta, 461:500.
Breton, J., and Vermeglio, A.,, 1982, Orientation of photosynthetic pigments in vivo, in: “Photosynthesis: Energy Conversion by Plants and Bacteria,” Govindjee, ed., v. 1, Academic Press, New York.
Clayton, R.K., 1965, Characteristics of fluorescence and delayed light emission from green photosynthetic bacteria and algae, J. Gen. Physiol., 48:633.
Fetisova, Z.G., and Borisov, A. Yu., 1980, Picosecond time scale of heterogeneous excitation energy transfer from accessory light-harvesting bacterioviridin antenna to main bacteriochtorophy11 a antenna in photoactive pigment-protein complexes obtained from Chlorobium limicola, a green bacterium, FEBS Lett., 114:323.
Fetisova, Z.G., and Fok, M.V., 1984, The ways of optimization of light energy conversion in primary steps of photosynthesis. I. Necessity of photosynthetic unit structure optimization and the method of its efficiency calculation (in Russian), Molek. Biol., 18:1651.
Fetisova, Z. G., Fok, M. M., and Shibaeva, L. V., 1985, The ways of optimization of light energy conversion in primary steps of photosynthesis. VI. The principles of organization of optimal artificial tight-harvesting molecular systems (in Russian), Molek. Biol., 19:1489.
Fetisova, Z.G., Kharchenko, S.G., and Rbdurakhmanov, I.A., 1986, Strong orientational ordering of the near-infrared transition moment vectors of light-harvesting antenna bacterioviridin in chromatophores of the green photosynthetic bacterium Chlorobium limicola, FEBS Lett., 199:234.
Fetisova, Z.G., Kharchenko, S.G., and Rbdurakhmanov, I.A., 1987, in: “Progress in Photosynthesis Research,” J. Biggins, ed., v. 1, Martinus Nijhoff, Dordrecht.
Freiberg, R.M., 1966, Primary processes of photosynthesis studied by fluorescence spectroscopy methods, Laser Chem. 6:233.
Govindjee, Hammond, J.H., and Merkelo, H., 1972, Lifetime of the excited state in vivo. II. BacteriochlorophytI in photosynthetic bacteria at room temperature, Biophys. J., 12:609.
Knox, R. 5., and van Metter, R.L., 1979, Fluorescence of tight-harvesting chlorophyll a/b protein complexes: implications for the photosynthetic unit, in: “Chlorophyll Organization and Energy Transfer in Photosynthesis,” Ciba Foundation Symposium 61 (new series), Excerpta Medica, Amsterdam.
Kondrat’eva, E. N., 1963, “The Photosynthetic Bacteria” (in Russian), Read. Sci. USSR Press, Moscow.
Olson, J.M., 1960, Chlorophyll organization in green photosynthetic bacteria, Biochim. Biophys. Acta, 594:33.
Swarthoff, T., Gast, P., Hoff, A.J., and Amesz, J., 1981a, An optical and ESR investigation on the acceptor side of the reaction center of the green photosynthetic bacterium Prosthecochloris aestuarii, FEBS Lett., 130:93.
Swarthoff, T., van der Veek-Horstey, K. M., and Amesz, J., 1981b, The primary charge separation, cytochrome oxidation and triplet formation in preparations from the green photosynthetic bacterium Prosthecochloris aestuarii, Biochim. Biophys. Acta, 635:1.
Sybesma, C., and Olson, J.M., 1963, Transfer of chlorophyll excitation energy in green photosynthetic bacteria, Proc. Natl. Read. Sci. U.S.A., 49:246.
Sybesma, C., and Mredenberg, W.J., 1963, Evidence for a reaction center P640 in the green photosynthetic bacterium Chloropseudomonas ethylicum, Biochim. Biophys. Acta, 75:439.
van Dorssen, R. J., Gerola, P. D., Olson, J. M., and Amesz, J., 1966a, Optical and structural properties of chlorosomes of the photosynthetic green sulfur bacterium Chlorobium limicola, Biochim. Biophys. Acta, 646:77.
van Dorssen, R.J., Masmel, H., and Amesz, J., 1966b, Pigment organization and energy transfer in the green photosynthetic bacterium Chloroftexus aurantiacus, Photosynth. Res., 9:33.
Vos, M., Nuijs, R. M., van Grondelle, R., van Dorssen, R. J., Gerola, P. D., and Amesz, J., 1967, Excitation transfer in chlorosomes of green photosytnehtic bacteria, Biochim. Biophys. Acta, 691:275.
Wechsler, T., Suter, F., Fuller, R.C., and Zuber, H., 1984, The complete amino acid sequence of the bacteriochlorophyll c-binding popypeptide from chlorosmes of the green photosynthetic bacterium Chloroflexus aurantiacus, FEBS Lett., 181:173.
Zuber, H., 1965, Structure and function of light-harvesting complexes and their polypeptides, Photochem. Photobiol., 42:821.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Plenum Press, New York
About this chapter
Cite this chapter
Freiberg, A.M., Timpman, K.E., Fetisova, Z.G. (1988). Excitation Energy Transfer in Living Cells of the Green Bacterium Chlorobium Limicola Studied by Picosecond Fluorescence Spectroscopy. In: Olson, J.M., Ormerod, J.G., Amesz, J., Stackebrandt, E., Trüper, H.G. (eds) Green Photosynthetic Bacteria. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1021-1_10
Download citation
DOI: https://doi.org/10.1007/978-1-4613-1021-1_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-8296-9
Online ISBN: 978-1-4613-1021-1
eBook Packages: Springer Book Archive