Choice, Preparation and use of Culture Medium
When selecting the proper media for human in vitro fertilization and embryo transfer (IVF-ET), a variety of physiological and biochemical parameters must be considered. Preinsemination oocyte (or egg) culturing and incubation of sperm under conditions conducive to motility maintenance, capacitation and the acrosome reaction are essential for successful sperm-egg interaction. Once fertilization has occurred, proper incubation conditions must be met to support early preimplantation development. Although there is much yet to learn about the optimal conditions for oocyte, egg, sperm and embryo culturing in human IVF-ET, a number of criteria have been shown to be essential for success. These criteria will be addressed only briefly here, and the reader is encouraged to examine several excellent reviews (Edwards, 1980; Mastroianni and Biggers, 1981; Wolf and Quigley, 1984) and Chapter 18 in this book dealing with the role of environmental factors on embryonic development.
KeywordsHepatitis Albumin Lactate Heparin Penicillin
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- Edwards, R. G., 1980, Conception in the Human Female, Academic Press, New York, 1087 pp.Google Scholar
- Mann, T. and Lutwak-Mann, C. (eds.), 1981, Male Reproductive Function and Semen, Springer-Verlag, New York, 495 pp.Google Scholar
- Mastroianni, L., Jr., and Biggers, J. D. (eds.), 1981, Fertilization and Embryonic Development In Vitro, Plenum Press, New York, 371 pp.Google Scholar
- Wolf, D. P. and Quigley, M. M. (eds.), 1984, Human In Vitro Fertilization and Embryo Transfer, Plenum Press, New York, 440 pp.Google Scholar