Abstract
Galili et al. 1 recently described the presence of a natural anti-agalactosyl IgG antibody (a-Gal-Ab) in the serum of all normal subjects. This antibody specifically recognizes terminal Gal a(l→3) structures2. In auto immune thyroid disorders, an overall B cell activation occurs, as reflected by the appearance of various IgG autoantibodies. We have therefore tested the possibility that a-Gal-Ab titers could be enhanced in those diseases. Using an indirect immunofluorescence technique, we previously showed the binding of purified a-Gal-Ab to the surface of trypsinized thyroid cells3. In the present work, we purify a thyroid glycopeptidic fraction which recognizes the a-Gal-Ab.
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References
U. Galili, E.A. Rachmilewitz, A. Peleg, I. Flechner, A unique natural human IgG antibody with anti-a-galactosyl specificity, J. Exp. Med. 160:1519 (1984).
U. Galili, B.A. Macher, S.B. Shohet, Human natural anti-α-galactosyl IgG. The specific recognition of α(l→3)-linked galactose residues, J. Exp. Med. 162:573 (1985).
J. Etienne-Decerf, M. Malaise, P. Mahieu, R. Winand, Elevated anti-agalactosyl antibody titers : a marker of progression in autoimmune thyroid disorders and in endocrine ophthalmopathy? Acta Endocr. In Press.
M.G. Malaise, J.C. Davin, P. Mahieu, P. Franchimont, Elevated antigalactosyl antibody titers reflect a renal injury after gold or D. Penicillamine in rhumatoid arthritis. Clin. Immunol. Immunopathol. 40:356 (1986).
B.C. Kress, R.G. Spiro, Studies on the glycoprotein nature of the thyrotropin receptor : interaction with lectins and purification of the bovine protein with the use of Bandeiraea (Griffonia) Simplicifolia I affinity chromatography, Endocrinology 118:974 (1986).
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© 1987 Plenum Press, New York
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Etienne-Decerf, J., Mahieu, P., Malaise, M., Winand, R. (1987). Measurement of Anti-α-Galactosyl Antibodies in the Course of Various Thyroid Disorders and Isolation of an Antigenic Glycopeptide Fraction. In: Pinchera, A., Ingbar, S.H., McKenzie, J.M., Fenzi, G.F. (eds) Thyroid Autoimmunity. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0945-1_39
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DOI: https://doi.org/10.1007/978-1-4613-0945-1_39
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