Further Characterization of the Thyroid Microsomal Antigen by Monoclonal Antibodies

  • Luc Portmann
  • Noboru Hamada
  • Wilfred A. Franklin
  • Leslie J. DeGroot

Abstract

The thyroid microsomal antigen has been recently identified as a 107 kD protein (1) by Western blot and immunoprecipitation using sera of patients with thyroid autoimmune disease. In addition other studies have reported the presence of antibodies in those sera against human thyroid peroxidase (2), and a coelution during gel filtration of peroxidase enzymatic activity and microsomal antigenicity (3) suggesting an identity between microsomal antigen and thyroid peroxidase. We developed monoclonal antibodies against this 107 kD antigen to better characterize its nature and relationship to thyroid peroxidase.

Keywords

Cellulose Peroxi Filtration Electrophoresis Polypeptide 

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Luc Portmann
    • 1
  • Noboru Hamada
    • 1
  • Wilfred A. Franklin
    • 2
  • Leslie J. DeGroot
    • 1
  1. 1.Thyroid Study Unit, Department of MedicinePathology The University of ChicagoChicagoUSA
  2. 2.Pathology The University of ChicagoChicagoUSA

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