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Primary Culture Systems for Mammary Biology Studies

  • Chapter
Cellular and Molecular Biology of Mammary Cancer

Abstract

The importance of hormones in the growth, morphogenesis, differentiation and neoplastic transformation of the mammary gland is well recognized due to a large number of experimentsin vivo (for review see 1). However, the precise nature of hormonal involvement is not clearly understood because of the difficulties in analyzing complex in vivo interactions. The role of growth factors in any of these processes is, perhaps, even less well understood, due in part to the complexity ofin vivo interactions and in part to the general lack of knowledge of the role of growth factors in anyin vivo process. The recent advances relating some viral oncogene products to growth factor receptor variants or growth factor sub-units makes it important to understand more fully the role of these molecules in the processes listed above. Culture systems of various types have been used to try and address these questions and are, theoretically, of great advantage since they allow observations and manipulations of cells and tissues in isolation, where experimental variables can be controlled or minimized. The goal of these investigations is to be able to reproduce in culture a system that mimics the in vivo physiological and pathological phemomena of interest. No individual system completely meets this goal, but recent advances allow a better simulation of some of the processes that occurin vivo. Unfortunately, even those systems which closely mimic the in vivo situation in terms of growth and differentiation cannot yet mimic the process of neoplastic transformation in culture.

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Authors and Affiliations

  1. Cancer Research Laboratory 3510 Life Sciences Building, University of California, Berkeley, CA, 94720, USA

    Brett K. Levay-Young, Walter Imagawa, Jason Yang, James E. Richards, Raphael C. Guzman & Satyabrata Nandi

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  1. Brett K. Levay-Young
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  2. Walter Imagawa
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  3. Jason Yang
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  4. James E. Richards
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  5. Raphael C. Guzman
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  6. Satyabrata Nandi
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Editors and Affiliations

  1. Baylor College of Medicine, Houston, Texas, USA

    Daniel Medina

  2. National Cancer Institute, Bethesda, Maryland, USA

    William Kidwell  & Elizabeth Anderson  & 

  3. Michigan Cancer Foundation, Detroit, Michigan, USA

    Gloria Heppner

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© 1987 Plenum Press, New York

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Levay-Young, B.K., Imagawa, W., Yang, J., Richards, J.E., Guzman, R.C., Nandi, S. (1987). Primary Culture Systems for Mammary Biology Studies. In: Medina, D., Kidwell, W., Heppner, G., Anderson, E. (eds) Cellular and Molecular Biology of Mammary Cancer. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0943-7_11

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  • DOI: https://doi.org/10.1007/978-1-4613-0943-7_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-42761-9

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