Resolution of Isoelectric Focusing
In this chapter, we study the nature of the behavior of amphoteric substances in a medium with a specified pH profile. We consider a multi-component mixture in the case where we may neglect reaction between the components forming the pH gradient and the substances subject to isoelectric focusing, which, for the electrophoresis model used, inevitably leads to exclusion of reactions between the substances to be focused. This is connected with the fact that the reactions are taken into account in terms of the total charge of the mixture and the electric current density, in which are included the charge and the current of the component creating the pH gradient. In this case, it is impossible to separate the reactions between the individual substances to be focused and the reactions between these substances and the pH gradient.
KeywordsThermal Diffusion Isoelectric Point Electric Current Density Laplace Method Separation Resolution
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