Abstract
Cytochemical labeling of intracellular components presupposes direct access to the cell interior. Traditionally, this access is rendered possible through sectioning of tissues or of cell pellets. Sectioning can be performed in fresh tissues, in polymer-embedded specimens, as well as in frozen sections. Alternative, although less conventional, methods do not involve cutting of the tissue but, instead, cleavage or fracture of previously frozen tissues.
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© 1990 Kluwer Academic Publishers
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Torrisi, M.R., Mancini, P., Da Silva, P.P. (1990). Cytochemical access to plasma and intracellular membranes of freeze-fractured hepatocytes and salivary gland cells. In: Riva, A., Motta, P.M., Riva, F.T. (eds) Ultrastructure of the Extraparietal Glands of the Digestive Tract. Electron Microscopy in Biology and Medicine, vol 6. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0869-0_9
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DOI: https://doi.org/10.1007/978-1-4613-0869-0_9
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