Intercellular junctions in salivary glands

  • Guido Fumagalli
  • Masaki Shimono
Part of the Electron Microscopy in Biology and Medicine book series (EMBM, volume 6)


Human and mammalian salivary glands are made up of combinations of acinar, ductal, and myoepithelial cells, with each cell type playing a defined role in salivary secretion. Thus, acinar cells are devoted to production and release of proteins and electrolytes, duct cells constitute the walls of the pipes that convey secreted material from acini to the oral cavity, and myoepithelial cells facilitate the movement of the secreted material. In these cells three types of intercellular junctions are present: tight junctions, gap junctions, and desmosomes.


Salivary Gland Tight Junction Intercellular Junction Acinic Cell Carcinoma Desmosomal Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    De Camilli P, Peluchetti D, Meldolesi J: Dynamic changes of the luminal plasmalemma in stimulated parotid acinar cells. J Cell Biol 70: 59–74, 1976.PubMedCrossRefGoogle Scholar
  2. 2.
    Mazariengos MR, Tice LW, Hand AR: Alteration of tight junctional permeability in the rat parotid gland after isoproterenol stimulation. J Cell Biol 98: 1865–1877, 1984.CrossRefGoogle Scholar
  3. 3.
    Inoue T, Yamane H, Yamamura T, Shimono M: Morphological changes of intercellular junctions in the rat submandibular gland treated by long-term repeated administration of isoproterenol. J Dent Res 66: 1303–1309, 1987.PubMedCrossRefGoogle Scholar
  4. 4.
    Shimono M, Yamamura T, Fumagalli G: Intercellular junctions in salivary glands: Freeze-fracture and tracer studies of normal rat sublingual gland. J Ultrastruct Res 72: 286–299, 1980.PubMedCrossRefGoogle Scholar
  5. 5.
    Shimono M, Nishihara K, Yamamura T: Intercellular junctions in developing rat submandibular glands. (I) Tight junctions. J Electron Microsc 30: 29–45, 1981.Google Scholar
  6. 6.
    Simson JAV, Bank HL: Freeze-fracture and lead ion tracer evidence for a paracellular fluid secretory pathway in rat parotid glands. Anat Rec 208: 69–80, 1984.PubMedCrossRefGoogle Scholar
  7. 7.
    Mazariengos MR, Hand AR: Regulation of tight junctional permeability in the parotid gland by autonomic agonists. J Dent Res 63: 1102–1107, 1984.CrossRefGoogle Scholar
  8. 8.
    Claude P, Goodenough DA: Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia. J Cell Biol 58: 390–400, 1973.PubMedCrossRefGoogle Scholar
  9. 9.
    Hull BE, Staehelin LA: Functional significance of the variations in the geometrical organization of the tight junction networks. J Cell Biol 68: 688–704, 1976.PubMedCrossRefGoogle Scholar
  10. 10.
    Mollgard K, Malinowska DH, Saunders NR: Lack of correlation between tight junction morphology and permeability properties in developing choroid plexus. Nature 264: 293–294, 1976.CrossRefGoogle Scholar
  11. 11.
    Martin K, Burgen ASV: Changes in the permeability of the salivary gland caused by sympathetic stimulation and by catecholamines. J Gen Physiol 46: 225–243, 1962.PubMedCrossRefGoogle Scholar
  12. 12.
    Jansen JW, DePont JJ, Bonting SL: Transepithelial permeability in the rabbit pancreas. Biochim Biophys Acta 551: 95–108, 1979.PubMedCrossRefGoogle Scholar
  13. 13.
    Garrett JR, Klinger AH, Parsons PA: Permeability of canine submandibular glands to blood-borne horseradish peroxidase during parasympathetic secretion. J Exp Physiol 67: 31–39, 1982.Google Scholar
  14. 14.
    Yamane H, Yamamura T, Satoh T, Shimono M: Tight junctions in submandibular acinar cells during secretion (abstr). J Dent Res 65: 833, 1986.Google Scholar
  15. 15.
    Dunn J, Revel JP: Association of gap junctions with endoplasmic reticulum in rat parotid glands. Cell Tiss Res 238: 589–594, 1984.CrossRefGoogle Scholar
  16. 16.
    Friend DS, Gilula NB: Variations in tight and gap junctions in mammalian tissues. J Cell Biol 53: 758–776, 1972.PubMedCrossRefGoogle Scholar
  17. 17.
    Nagato T, Tandler B: Gap junctions in rat sublingual gland. Anat Rec 214: 71–75, 1986.PubMedCrossRefGoogle Scholar
  18. 18.
    Goodenough DA, Revel JP: A fine structural analysis of intercellular junctions in the mouse liver. J Cell Biol 45, 272–290, 1970.PubMedCrossRefGoogle Scholar
  19. 19.
    Peracchia C: Gap junctions. Structural changes after uncoupling procedures. J Cell Biol 72: 628–641, 1977.PubMedCrossRefGoogle Scholar
  20. 20.
    Staehelin LA: Structure and function of intercellular junctions. Int Rev Cytol 39: 191–283, 1974.PubMedCrossRefGoogle Scholar
  21. 21.
    Shimono M, Clementi F: Intercellular junctions of oral epithelium. I. Studies with freeze-fracture and tracing methods of normal rat keratinized oral epithelium. J Ultrastruct Res 56: 121–136, 1976.PubMedCrossRefGoogle Scholar
  22. 22.
    Simpson I, Rose B, Loewenstein WR: Size limit of molecules permeating the functional membrane channels. Science 195: 294–296, 1977.PubMedCrossRefGoogle Scholar
  23. 23.
    Hertzberg EL, Gilula NB: Isolation and characterization of gap junctions from rat liver. J Biol Chem 254: 2138–2147, 1979.PubMedGoogle Scholar
  24. 24.
    Nicholson BJ, Gros DB, Kent SBH, Hoos LE, Revel JP: The Mr 28,000 gap junction protein from rat heart and liver are different but related. J Biol Chem 260: 6514–6517, 1985.PubMedGoogle Scholar
  25. 25.
    Paul D: Molecular cloning of cDNA from rat liver gap junction protein. J Cell Biol 103: 123–134, 1986.PubMedCrossRefGoogle Scholar
  26. 26.
    Kumar M, Gilula NB: Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein. J Cell Biol 103: 767–776, 1986.PubMedCrossRefGoogle Scholar
  27. 27.
    Young JD, Cohn ZA, Gilula NB: Functional homology of gap junction conductance in lipid bilayer: Demonstration that the major 27 Kd protein forms the junctional channel. Cell 48: 733–743, 1987.PubMedCrossRefGoogle Scholar
  28. 28.
    Nicholson B, Dermietzel R, Teplow D, Traub O, Wittecke K, Revel JP: Two homologous protein components of hepatic gap junctions. Nature 329: 732–733, 1987.PubMedCrossRefGoogle Scholar
  29. 29.
    Obaid AL, Socolar SJ, Rose B: Cell to cell channels with two independently regulated gates in series: Analysis of functional conductance modulation by membrane potential, calcium and pH. J Membr Biol 73: 69–89, 1983.PubMedCrossRefGoogle Scholar
  30. 30.
    Unwin PMT, Ennis PD: Calcium-mediated changes in gap junction structure: Evidence from the low angle X-ray pattern. J Cell Biol 97: 1459–1466, 1983.PubMedCrossRefGoogle Scholar
  31. 31.
    Meda P, Perrelet A, Orci L: Increase of gap junctions between pancreatic B-cell during stimulation of insulin secretion. J Cell Biol 82: 441–448, 1979.PubMedCrossRefGoogle Scholar
  32. 32.
    Kelly DE, Shienvold FL: The desmosome: Fine structure with freeze-fracture replication and tannic acid staining of sectioned epidermis. Cell Tiss Res 172: 309–323, 1976,CrossRefGoogle Scholar
  33. 33.
    Penn EJ, Hobson C, Rees DA, Magee AI: Structure and assembly of desmosome junctions: Biosynthesis, processing and transport of the major protein and glycoprotein components in cultured epithelial cells. J Cell Biol 105: 57–68, 1987.PubMedCrossRefGoogle Scholar
  34. 34.
    Jones JCR, Goldman RD: Intermediate filaments and the initiation of desmosome assembly. J Cell Biol 101: 506–517, 1985.PubMedCrossRefGoogle Scholar
  35. 35.
    Mattey DL, Garrod DR: Mutual desmosome formation between all binary combination of human, bovine, canine, avian and amphibian cells: Desmosome formation is not tissue or species specific. J Cell Sci 75: 377–399, 1985.PubMedGoogle Scholar
  36. 36.
    Giudice GJ, Cohen SM, Patel NH, Steinberg MS: Immunological comparison of desmosomal components from several bovine tissues. J Biol Chem 26: 35–45, 1984.Google Scholar
  37. 37.
    Penn EJ, Burdett IDJ, Hobson C, Magee AI, Rees DA: Structure and assembly of desmosome junctions: Biosynthesis and turnover of the major desmosome components of Madin-Darby canine kidney cells in low calcium medium. J Cell Biol 105: 2327–2334, 1987.PubMedCrossRefGoogle Scholar
  38. 38.
    Cowin P, Mattery D, Garrod DR: Identification of desmosomal surface components (desmocollins) and inhibition of desmosome formation by specific fab. J Cell Sci 70: 41–60.Google Scholar
  39. 39.
    Jones JCR, Arnn J, Staehelin LA, Goldman RD: Human autoantibodies against desmosomes: Possible causative factors in pemphigus. Proc Natl Acad Sci USA 81: 2781–2785, 1984.PubMedCrossRefGoogle Scholar
  40. 40.
    Cutler LS, Chaudhry AP: Intercellular contacts at the epithelial-mesenchymal interface during the prenatal development of the rat submandibular gland. Develop Biol 33: 229–240, 1973.PubMedCrossRefGoogle Scholar
  41. 41.
    Cutler LS, Chaudhry AP: Cytodifferentiation of the acinar cells of the rat submandibular gland. Develop Biol 41: 31–41, 1974.PubMedCrossRefGoogle Scholar
  42. 42.
    Cutler LS, Chaudhry AP: Cytodifferentiation of striated duct cells and secretory cells of the convoluted granular tubules of the rat submandibular gland. Am J Anat 143: 201–217, 1975.PubMedCrossRefGoogle Scholar
  43. 43.
    Redman RS, Sreebny LM: Proliferative behavior of differentiating cells in the developing rat patotid gland. J Cell Biol 46: 81–87, 1970.PubMedCrossRefGoogle Scholar
  44. 44.
    Montesano R, Friend DS, Perrelet A, Orci L: In vivo assembly of tight junctions in fetal rat liver. J Cell Biol 61: 310–319, 1975.CrossRefGoogle Scholar
  45. 45.
    Luciano L, Thiele J, Reale E: Development of follicles and of occluding junctions between the follicular cells of the thyroid gland. J Ultrastruct Res 66: 164–181, 1979.PubMedCrossRefGoogle Scholar
  46. 46.
    Inoue T, Shimono M, Yamamura T, Saito I, Watanabe O, Kawahara H: Acinic cell carcinoma arising in the glossopalatine glands: A report of two cases with electron microscopic observations. Oral Surg Oral Med Oral Pathol 57: 398–407, 1984.PubMedCrossRefGoogle Scholar
  47. 47.
    Lucas RB: Pathology of Tumours of the Oral Tissues, 4th ed. Edinburgh: Churchill Livingstone, 1984.Google Scholar
  48. 48.
    Shimono M, Sato T, Yamane H, Katayanagi T, Osada K, Watanabe I, Yamane G, Noma H: Tight junctions in an undifferentiated carcinoma originating in the parotid gland: A study with freeze-fracture. Bull Tokyo Dent Coll 28: 63–73, 1987.PubMedGoogle Scholar
  49. 49.
    Alroy J, Banner BF, Pauli BU, Leav I: Alterations of intercellular junctions in acinic cell carcinoma of the canine pancreas. Virchows Arch (Cell Pathol) 28: 21–30,1978.Google Scholar
  50. 50.
    Banner BF, Alroy J, Pauli BU, Carpenter JL: An ultrastructural study of acinic cell carcinomas of the canine pancreas. Am J Pathol 93: 165–182, 1978.PubMedGoogle Scholar
  51. 51.
    Kerjaschki D, Krisch K, Sleyter UB, Umrath W, Jakesz R, Depisch R, Kokoschka R, Hörandner H: The structure of tight junctions in human thyroid tumors. Am J Pathol 96: 207–226, 1979.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Guido Fumagalli
  • Masaki Shimono
    • 1
  1. 1.Department of PathologyTokyo Dental CollegeChiba-City, ChibaJapan

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