Evaluation of an In Vitro Dual Perfusion System for the Study of Placental Proteins: Energy Metabolism

  • H. Schneider
  • A. Malek
  • R. Duft
  • N. Bersinger


The in vitro perfusion of human placenta offers several attractions to the investigator of placental function. Species differences which must be considered when data from animal experiments are discussed are of no concern and there are no ethical or safety questions. Furthermore the isolated in vitro situation allows the controlled modification of various experimental conditions. As in other in vitro techniques — for example, slices or membrane preparations — the tissue function is being studied after isolation from its natural environment, so considerable differences from in vitro behaviour are likely. Maternal and fetal influences which affect placental function by continuously modifying the “perfusate” are excluded, which is advantageous but at the same time present limitations in the interpretation of data.


Vaginal Delivery Glucose Consumption Lactate Production Human Placenta Iodoacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cédard, L. (1971) Placental perfusion in vitro. In: “Perfusion techniques”. Editor: E. Diczfalusy. 4th Karolinska Symposium, pp 331– 346, New York.Google Scholar
  2. 2.
    Panigel, M. (1969) Placental perfusion. In: “Fetal homeostasis”. Editor: R. Wynn. Vol. 4. Appleton Century Crofts, p 15, New York.Google Scholar
  3. 3.
    Schneider, H. and Dancis, J. (1985) In vitro perfusion of human placental tissue. International Workshop, Zurich 1984, Karger, Basel.Google Scholar
  4. 4.
    Bergmeyer, H.V. (1974) Methods of enzymatic analysis. 2nd Edition, Weinheim: Verlag Chemie, New York: Academic Press.Google Scholar
  5. 5.
    Schneider, H. and Huch, A. (1985) Dual in vitroperfusion of an isolated lobe of human placenta: Method and instrumentation. In: “In vitroperfusion of human placental tissue”. Editors: H. Schneider and J. Dancis. S. Karger, Basel.Google Scholar
  6. 6.
    Folkman, J., Cole, P. and Zimmerman, S. (1966) Tumour behaviour in isolated perfused organs in vitro, growth and metastases of biopsy material in rabbit thyroid and canine intestinal segment. Ann. Surg. 164, 491.PubMedCrossRefGoogle Scholar
  7. 7.
    Brodie, B.B., Axelbrod, J., Soberman, R. and Levi, B.B. (1949) The estimation of antipyrine in biological materials. J. Biol. Chem. 179, 25 – 29.Google Scholar
  8. 8.
    Heinegard, D. and Tiderstrom, G. (1973) Determination of serum creatinine by a direct colorimetric method. Clin. Chim. Acta. 43, 305.PubMedCrossRefGoogle Scholar
  9. 9.
    Kreisberg, R.A., Siegal, A.N. and Owen, W.C. (1972) Allanine und gluconeogenesis in men: Effect of ethanol. J. Clin. Endocrinol. 34, 876 – 883.CrossRefGoogle Scholar
  10. 10.
    Soda, R.J., Proegler, M. and Schneider, H. (1984) Transfer and metabolism of norepinephrine studied from maternal to fetal and fetal to maternal sides in the in vitroperfused human placental lobe. Am. J. Obstet. Gynecol. 148, 474 – 481.Google Scholar
  11. 11.
    Challier, J.C., Schneider, H. and Dancis, J. (1976) In vitroperfusion of human placenta. V. Oxygen consumption. Am. J. Obstet. Gynecol. 126, 261 – 265.PubMedGoogle Scholar
  12. 12.
    Bersinger, N.A., Malek, A., Benz, B., Keller, P.J. and Schneider, H. (1988) Effect of protein synthesis inhibitors and metabolic blockers on the production of placental proteins by the in vitroperfused human placenta. Gynecol. Obstet. Invest. 25, 145 – 151.PubMedCrossRefGoogle Scholar
  13. 13.
    Carroll, M.J. and Young M. (1987) Observations on the energy and redox state on protein synthetic rate in animal and human placentas. J. Perinat. Med. 15, 21 – 30.PubMedCrossRefGoogle Scholar
  14. 14.
    Carroll, M.J. and Young M. (1982) Mixed protein synthetic rate in the tissue of the isolated lobule of the human placenta. J. Physiol. 332, 5.Google Scholar
  15. 15.
    Bloxam, D.C. and Bobinski, P.M. (1984) Energy metabolism and glycolysis in the human placenta during ischaemia and in normal labour. Placenta 5, 381–394. Google Scholar
  16. 16.
    Harkness, R.A., Coade, S.B., Simmonds, R.J. and Duffy, S. (1985) Effect of a failure of energy supply on adenine nucleotide breakdown in placentae and other fetal tissues from rat and guinea pig. Placenta199 – 216.Google Scholar
  17. 17.
    Holzmann, J.R., Philipp, A.F. and Battaglia, F.C. (1979) Glucose metabolism, lactate and ammonia production by the human placenta in vitro. Pediatr. Res. 13, 17.Google Scholar
  18. 18.
    Schneider, H., Challier, J.C. and Dancis, J. (1981) Transfer and metabolism of glucose and lactate in the human placenta studied by a perfusion system in vitro. Placenta(Suppl 2.), 129 – 138.Google Scholar
  19. 19.
    Burd, L.J., Jones, M.D., Simmons, M.A., Makowski, E.L., Meschia, G. and Battaglia, F.C. (1975) Placental production and fetal utilization of lactate and pyruvate. Nature 254, 710 – 711.PubMedCrossRefGoogle Scholar
  20. 20.
    Warburg, O. (1925) The metabolism of carcinoma cells. Am. J. Canc. IX, 148 – 163.Google Scholar
  21. 21.
    Sweeney, M.J., Ashmore, J., Morris, H.P. and Weber, G. (1963) Comparative biochemistry of hepatomas. IV. Isotope studies of glucose and fructose metabolism in liver tumours of different growth rates. Canc. Res. 23, 995–1002.Google Scholar
  22. 22.
    Penfold, P., Illsley, N.P., Purkiss, P. and Jennings, P. (1984) Human placental amino acid transfer and metabolism in oxygenated and anoxic conditions. Tropbobl. Res. 1, 27 – 36.Google Scholar
  23. 23.
    Sakuray, T., Takagi, H. and Hosoya, N. (1969) Metabolic pathways of glucose in human placenta: Changes with gestation and with added 17 estradiol. Am. J. Obstet. Gynecol. 105, 1044 – 1054.Google Scholar
  24. 24.
    Lopez-Bernal, A. (1984) Corticosteroid metabolism by human intrauterine tissues in relation to parturition. D. Phil. Thesis, University of Oxford.Google Scholar
  25. 25.
    Challier, J.C., Hauguel, S. and Desmaizières, V. (1985) Metabolism and transfer of radioactive glucose in the human placenta studied by dual perfusion. Contrib. Gynec. Obstet. 13, 144 – 146.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • H. Schneider
    • 1
  • A. Malek
    • 1
  • R. Duft
    • 1
  • N. Bersinger
    • 2
  1. 1.University of BerneSwitzerland
  2. 2.University of ZurichSwitzerland

Personalised recommendations