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Overproduction of Proteins by Recombinant DNA: Human Insulin

  • Chapter
Cell Function and Disease

Abstract

An important goal of recombinant DNA research is to develop methods for the overproduction of useful gene products. Cloning a gene of interest for its expression in a particular host system is not an easy task, for the manipulations require extensive knowledge of the regulatory mechanisms of gene expression, basic understanding of the vector-host relationships, skills in the recombinant DNA techniques, adequate technical support for cell growth, and finally, suitable purification procedures for the protein products of interest.

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Author information

Authors and Affiliations

  1. Centro de Investigación sobre Ingenieria Genetica y Biotecnologia, Universidad Nacional Autonoma de Mexico, Apdo Postal 510-3, Cuernavaca Mor., C.P. 62270, Mexico

    Paulina Balbas, Ramon de Anda, Noemi Flores, Xochitl Alvarado, Norberto Cruz, Fernando Valle & Francisco Bolivar

Authors
  1. Paulina Balbas
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  2. Ramon de Anda
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  3. Noemi Flores
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  4. Xochitl Alvarado
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  5. Norberto Cruz
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  6. Fernando Valle
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  7. Francisco Bolivar
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Editor information

Editors and Affiliations

  1. Department of Biochemistry School of Medicine, University of Nuevo Leon, Monterrey, Nuevo Leon, Mexico

    L. E. Cañedo

  2. Magnetic Resonance Unit, University Hospital, Monterrey, Nuevo Leon, Mexico

    L. E. Todd

  3. Lawrence Berkeley Laboratory, University of California, Berkeley, California, USA

    L. Packer

  4. International Biomedical Institute, Bari, Italy

    J. Jaz

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© 1988 Plenum Press, New York

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Balbas, P. et al. (1988). Overproduction of Proteins by Recombinant DNA: Human Insulin. In: Cañedo, L.E., Todd, L.E., Packer, L., Jaz, J. (eds) Cell Function and Disease. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0813-3_5

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  • DOI: https://doi.org/10.1007/978-1-4613-0813-3_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8095-8

  • Online ISBN: 978-1-4613-0813-3

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