Enzyme-Linked Immunosorbent Assay and Related Sensitive Assays for Antigen and Antibody Detection

  • Jean-Luc Guesdon
  • Zoilo Pires de Camargo
  • Edouard Drouhet
  • Stratis Avrameas

Abstract

A great deal of effort in the past decade has been directed to the development of rapid, sensitive and accurate methods for detection of antigens of pathogenic microorganisms or the corresponding antibodies. Most of the current rapid tests utilize polyclonal or monoclonal antibodies or antigens labelled with a marker which can be easily quantified with high sensitivity. The labelling of the antigen or the antibody can be achieved with substances such as a radioactive isotope, free radicals, a fluorescent dye, a bacteriophage or an enzyme. In comparison with radioisotope, spin, bacteriophage, and fluorescent dye labelling, the use of enzyme as marker has several interesting features: the reagents are stable for years, possess intrinsic amplification, and there are no problems of health hazards for laboratory personnel or waste disposal when appropriate enzymes and substrates are selected.

Keywords

Starch Cortisol Immobilization Polystyrene Disulfide 

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Jean-Luc Guesdon
    • 1
  • Zoilo Pires de Camargo
    • 2
  • Edouard Drouhet
    • 2
  • Stratis Avrameas
    • 1
  1. 1.Immunocytochemistry UnitPasteur InstituteParisFrance
  2. 2.Mycology UnitPasteur InstituteParisFrance

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