Isotope Dilution — Mass Spectrometry as a Reference Method

  • L. Siekmann
  • R. Kruse
  • G. Röhle

Abstract

The accuracy of clinical chemical testing is today evaluated by means of both internal and external quality control using appropriate control sera. For each analyte in a control material more than one analytical method exists and, unfortunately, the results of various methods and of various test kits applied to one and the same control material differ to a greater or lesser extent. This situation is particularly untenable considering the fact that only one value e.g. creatinine concentration in serum can be the “true” one. It is obvious that any progress towards improving the comparability of analytical results from different laboratories is hampered as long as methods with a known or unknown bias are accepted.

Keywords

Cholesterol Glycerol Corn Creatinine Cortisol 

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References

  1. 1.
    Hoppen HO, Siekmann L, Breuer H. Identifizierung von Aminosäure- und Peptidconjugaten von Oestrogenen mit Hilfe der Massenspektrometrie. Z Anal Chem 1970; 252: 299–304.CrossRefGoogle Scholar
  2. 2.
    Siekmann L. Determination of creatinine in human serum by isotope dilution-mass spectrometry. J Clin Chem Clin Biochem 1985; 23: 137–44.Google Scholar
  3. 3.
    Siekmann L. Determination of steroid hormones by the use of isotope dilution-mass spectrometry: a definitive method in clinical chemistry. J Steroid Biochem 1979; 11: 117–23.CrossRefGoogle Scholar
  4. 4.
    Siekmann L. Determination of uric acid in human serum by isotope dilution-mass spectrometry. J Clin Chem Clin Biochem 1985; 23: 129–35.Google Scholar
  5. 5.
    Siekman L. Hüskes KP, Breuer H. Determination of cholesterol in serum using mass fragmentography — a reference method in clinical chemistry. Z Anal Chemie 1976; 279: 145–6.CrossRefGoogle Scholar
  6. 6.
    Siekmann L, Schönfelder A, Siekmann A. Isotope dilution-mass spectrometry of total glycerol in human serum — a reference method in clinical chemistry. Z Anal Chemie 1986; 324: 280–1.CrossRefGoogle Scholar
  7. 7.
    Siekmann L, Breuer H. Determination of Cortisol in human plasma by isotope dilution-mass spectrometry. J Chem Clin Biochem 1982; 20: 883–92.Google Scholar
  8. 8.
    Siekmann L. Determination of oestradiol-17β in human serum by isotope dilution-mass spectrometry. J Clin Chem Biochem 1984; 22: 551–7.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • L. Siekmann
    • 1
  • R. Kruse
    • 1
  • G. Röhle
    • 1
  1. 1.Institut für Klinische BiochemieUniversität BonnGermany

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