Isotope Dilution — Mass Spectrometry as a Reference Method
The accuracy of clinical chemical testing is today evaluated by means of both internal and external quality control using appropriate control sera. For each analyte in a control material more than one analytical method exists and, unfortunately, the results of various methods and of various test kits applied to one and the same control material differ to a greater or lesser extent. This situation is particularly untenable considering the fact that only one value e.g. creatinine concentration in serum can be the “true” one. It is obvious that any progress towards improving the comparability of analytical results from different laboratories is hampered as long as methods with a known or unknown bias are accepted.
KeywordsUric Acid Reference Method Activate Charcoal Control Material Serum Pool
Unable to display preview. Download preview PDF.
- 2.Siekmann L. Determination of creatinine in human serum by isotope dilution-mass spectrometry. J Clin Chem Clin Biochem 1985; 23: 137–44.Google Scholar
- 4.Siekmann L. Determination of uric acid in human serum by isotope dilution-mass spectrometry. J Clin Chem Clin Biochem 1985; 23: 129–35.Google Scholar
- 7.Siekmann L, Breuer H. Determination of Cortisol in human plasma by isotope dilution-mass spectrometry. J Chem Clin Biochem 1982; 20: 883–92.Google Scholar
- 8.Siekmann L. Determination of oestradiol-17β in human serum by isotope dilution-mass spectrometry. J Clin Chem Biochem 1984; 22: 551–7.Google Scholar