Spin Labeling pp 615-634 | Cite as

Approaches to the Chemical Synthesis of 15N and Deuterium Substituted Spin Labels

  • Jane H. Park
  • Wolfgang E. Trommer
Part of the Biological Magnetic Resonance book series (BIMR, volume 8)

Abstract

With the increasing application of EPR to an expanding field of biomedical problems, the importance of the design and synthesis of spin labels intensifies. The spin label itself can either significantly enhance the productivity of an investigation or constitute the limiting factor. As illustrated in Chapter 11, isotopic substitution of 15N and/or deuterium in the spin probes substantially improves the quality and quantity of information obtained in the areas of enzyme catalysis and membrane characterization. The same design principles apply to 14N,1H and 15N,2H spin labels; however, the syntheses of isotopically substituted compounds present special problems due to the expense of the isotopes and the stringent requirements for isotopic purity in the product. These problems are addressed in detail in Sections 2-4 below. In Chapter 11 and this Appendix, a circumscribed number of isotopically substituted spin labels have been selected to illustrate the potential for dynamic motional analyses. The scope of the presentation is limited in part by the number of papers available in this relatively new area of isotopic substitution and by the authors’ intent to focus on subjects of interest to the biomedical scientist and molecular biologist.

Keywords

Pyridine Oxalate Anhydride Adenine Dioxane 

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Jane H. Park
    • 1
  • Wolfgang E. Trommer
    • 2
  1. 1.Department of Molecular Physiology and BiophysicsVanderbilt University School of MedicineNashvilleUSA
  2. 2.Department of ChemistryUniversity of KaiserslauternKaiserslauternFederal Republic of Germany

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