In Vivo Metabolism of Apolipoproteins C-II Aid C-IIII in Normal and Hypertriglyceridemic Subjects

  • C. L. Malmendier
  • J.-F. Lontie
  • D. Dubois
  • C. Delcroix
  • T. Magot
  • L. De Roy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 243)


Two papers on apo C-II and apo C-III metabolism have been published in 1981 and 1982 (1,2), only one comparing normal and hypeirlipoproteinemic subjects (1). The kinetic analysis of 1 2 5l labeled VLDL was followed in plasma for 48 h only and there were no urine data. The essential limitation of these early studies was their short duration due to the low specific activity of each individual C peptide obtained after whole VLDL-apoprotein labeling (apo B, C, E) necessitating tedious and error-prone separation procedures.


Lipoprotein Lipase Synthetic Rate Lipid Research Clinic Human Apolipoprotein Fractional Catabolic Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    M.W. Huff, N.H. Fidge, P.J. Nestel, T. Billington, and B. Wat-son, Metabolism of C-apolipoproteins: kinetics of C-II, C-IIIi and C - III 2, and VLDL-apolipoprotein B in normal and hyperlipoproteinemic subjects, J. Lipid Res., 22:1235 (1981).PubMedGoogle Scholar
  2. 2.
    M.W. Huff, and P.J. Nestel, Metabolism of apolipoproteins CII, CIIIi and CI II 2 and VLDL-B in human subjects consuming high carbohydrate diets, Metabolism, 31:493 (1982).PubMedCrossRefGoogle Scholar
  3. 3.
    C.L. Malmendier, J-F. Lontie, G.A. Grutman, and C. Delcroix, Metabolism of apolipoprotein C-I in normolipoproteinemic human subjects, Atherosclerosis, 62:167 (1986)PubMedCrossRefGoogle Scholar
  4. 4.
    C.L. Malmendier, J-F. Lontie, G. Grutman, and C. Delcroix, Metabolism of apolipoprotein C: kinetic studies in human subjects: a critical review, In: Lipoproteins and Atherosclerosis, C.L. Malmendier,and P. Alaupovic, eds, Plenum Press, New York (1986).Google Scholar
  5. 5.
    C.L. Malmendier, J-F. Lontie, G.A. Grutman, and G. Delcroix, Metabolism of apolipoprotein C-III in normolipemic human subjects, Atherosclerosis, 6 9:51 (1988).CrossRefGoogle Scholar
  6. 6.
    M.L. Kashyap, L.S. Srivastava, B.A. Hynd, G. Perisutti, D.W. Brady, P. Gartside, and C.J. Glueck, The role of high density lipoprotein apolipoprotein CII in triglyceride metabolism, Lipids, 13:933 (1978).PubMedCrossRefGoogle Scholar
  7. 7.
    J.L. Goldstein, W.R. Hazzard, H.G. Schrott, E.L. Bierman, and A.G. Motulsky, Hyperlipidemia in coronary heart disease. I. Lipid levels in 500 survivors of myocardial infarction, J. Clin. Invest., 52:1533 (1973).PubMedCrossRefGoogle Scholar
  8. 8.
    J.L. Steinfeld, R.R. Paton, A.L. Flick, R.A. Milch, F.E. Beach, and D.L. Tabern, Distribution and degradation of human serum albumin labeled with I1 3 1 by different techniques, Ann. N.Y. Acad. Sci, 70: 109 (1957).PubMedCrossRefGoogle Scholar
  9. 9.
    Lipid Research Clinics Program. Manual of Laboratory Operations. Lipid and Lipoprotein Analysis (DHEW Publication N° (NIH) 75-628), National Institutes of Health, Bethesda, MD, 1974.Google Scholar
  10. 10.
    W.T. Friedewald, R.I. Levy, and D.S. Fredrickson, Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge, Clin. Chem., 18:499, (1972).PubMedGoogle Scholar
  11. 11.
    D.Y. Dubois, F. Cantraine, and C.L. Malmendier, Comparison of different sandwich enzyme immunoassays for the quantitation of human apolipoproteins A-I and A-II, J. Immunol. Methods, 96:115 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    J-C. Fruchart, C. Fievet, and P. Puchois, Apolipoproteins, in : Methods of Enzymatic Analysis, Vol. VIII, H.U. Bergmeyer, ed., Verlag Chemie, Weinheim (1985).Google Scholar
  13. 13.
    J. Bury, G. Michiels, and M. Rosseneu, Human apolipoprotein C-II quantitation by sandwich enzyme-linked immunosorbent assay, J. Clin. Chem. Clin. Biochem., 24:457 (1986).PubMedGoogle Scholar
  14. 14.
    J. Bury, and M. Rosseneu, Enzyme linked immunosorbent assay for human apolipoprotein C-III, J. Clin. Chem. Clin. Biochem., 23:63 (1985).PubMedGoogle Scholar
  15. 15.
    Y.C. Ha, and P.J. Barter, Rapid separation of plasma lipoproteins by gel permeation chromatography on agarose gel Superose 6B, J. Chromatogr., 341:154 (1985).PubMedCrossRefGoogle Scholar
  16. 16.
    C.M.E. Matthews, The theory of tracer experiments with iodine 131-labeled plasma proteins, Phys. Med. Biol., 2:36 (1957).PubMedCrossRefGoogle Scholar
  17. 17.
    A. Rescigno, and E. Gurpide, Estimation of average times of residence, recycle and interconversion of blood-borne coumpounds using tracer methods, J. Clin. Endocrinol. Metab., 36:263 (1973).PubMedCrossRefGoogle Scholar
  18. 18.
    C.L. Malmendier, and J-F. Lontie, Turnover studies of apolipoproteins C: a first critical appraisal, in: Proceed. 2nd Europ. Workshop on Lipid Metabolism, Munich (1988). Springer Verlag, Heidelberg, in press.Google Scholar
  19. 19.
    D.S. Fredrickson, J.L. Goldstein, and M.S. Brown, The familial hyperlipoproteinemias, chapter 30, in: The Metabolic Basis of Inherited Disease, J.B. Stanbury, J.B. Wyngaarden, and D.S. Fredrickson, eds., McGraw-Hill, New York (1978).Google Scholar
  20. 20.
    W.C. Breckenridge, J.A. Little, G. Steiner, A. Chow, and M. Poapst, Hypertriglyceridemia associated with deficiency of apolipoprotein C-II, N. Engl. J. Med., 298:1265 (1978).PubMedCrossRefGoogle Scholar
  21. 21.
    W.C. Breckenridge, Apolipoprotein C-II deficiencies: in vivo models for assessing the significance of defective lipolysis on lipoprotein metabolism, in: Eicosanoids, Apolipoproteins, Lipoprotein Particles and Hypertriglyceridemia, C.L. Malmendier, and P. Alaupovic, eds., Plenum Press (1988). This volume.Google Scholar
  22. 22.
    L.A. Carlson, and D. Ballantyne, Changing relative proportions of apolipoproteins CII and CIII of very low density lipoproteins in hypertriglyceridaemia, Atherosclerosis, 23:563 (1976).PubMedCrossRefGoogle Scholar
  23. 23.
    N.E. Miller, S.N. Rao, P. Alaupovic, N. Noble, J. Slack, J.D. Brunzell, and B. Lewis, Familial apolipoprotein CII deficiency: plasma lipoproteins and apolipoproteins in heterozygous and homozygous subjects and the effects of plasma infusion, Eur. J. Clin. Invest. 11:69 (1981).PubMedCrossRefGoogle Scholar
  24. 24.
    W.V. Brown, and M.L. Baginsky, Inhibition of lipoprotein lipase by an apoprotein of human very low density lipopro tein, Biochem. Biophys. Res. Comm., 46:375 (1972).PubMedCrossRefGoogle Scholar
  25. 25.
    E. Windier, and R.J. Havel, Inhibitory effects of C apolipoproteins from rats and humans on the uptake of tri glyceride-rich lipoproteins and their remnants by the perfused rat liver, J. Lipid. Res., 26:556 (1985).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • C. L. Malmendier
    • 1
  • J.-F. Lontie
    • 1
  • D. Dubois
    • 1
  • C. Delcroix
    • 1
  • T. Magot
    • 2
  • L. De Roy
    • 3
  1. 1.Fondation de Recherche sur l’AthéroscléroseBrusselsBelgium
  2. 2.Laboratoire de Physiologie de la NutritionUniversité de Paris-SudOrsayFrance
  3. 3.Hôpital MilitaireBrusselsBelgium

Personalised recommendations