T’ang and co-workers (1957), who had adapted Cox’s method (1938) for the growth of rickettsiae in embryonated hen’s eggs in order to isolate chlamydiae, also studied the susceptibility of C. trachomatis to certain antibiotics. They found chlamydiae to be resistant to aminoglycosides and susceptible to tetracyclines. It is noteworthy that one of the first observations made in the experimentally infected hen’s eggs was that penicillin affected the growth of chlamydiae. Two other chlamydial research pioneers, Gordon and Quan, also used embryonated hen’s eggs for antibiotic susceptibility studies in 1962. In order to improve the isolation technique for tissue cell cultures, they were able to demonstrate that such drugs as bacitracin, nystatin, and polymyxin had no antichlamydial effect and could be used to suppress bacterial overgrowth in chlamydial cultures.
KeywordsMinimum Inhibitory Concentration Antibiotic Susceptibility Minimum Bactericidal Concentration Bacterial Overgrowth Tissue Cell Culture
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