Chlamydiae are obligate intracellular parasites and therefore require living (eukaryotic) cells to reproduce. The earliest methods used to isolate chlamydiae (i.e., laboratory animals and embryonated hen’s eggs) were too insensitive, laborious, and expensive to be employed for large-scale use in routine diagnostic work. The first tissue cell culture used to recover C. trachomatis employed McCoy cells that were exposed to irradiation 5 days before use (Gordon and Quan, 1965). Irradiated cells are cumbersome to use, which limits their general application in clinical diagnostic laboratories.
KeywordsChlamydial Infection Iodine Staining Ophthalmia Neonatorum McCoy Cell Chlamydial Inclusion
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