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Time-Resolved Fluorimetry using Synchrotron Radiation and Maximum Entropy Method of Analysis

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Light in Biology and Medicine

Abstract

Unpolarised pulse fluorescence is being used increasingly in biophysics to study the fluctuations and/or heterogeneity in the three-dimensional structure of macromolecules. Polarised pulse fluorescence also gives a direct physical measurement of the Brownian rotational constants in solution of the whole molecule, deformations and flexions of the structure and local motions of side-chains around their chemical bonds. Both these aspects can be related to biological activity.

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References

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Author information

Authors and Affiliations

  1. LURE CNRS Bât.209D, Université Paris-Sud, 91405, Orsay Cedex, France

    Jean-Claude Brochon

  2. M.R.C. Laboratory of Molecular Biology, Hills Road and DAMTP Silver Street, Cambridge, UK

    Alastarir Livesey

Authors
  1. Jean-Claude Brochon
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  2. Alastarir Livesey
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Editor information

Editors and Affiliations

  1. The City University, London, UK

    Ron H. Douglas

  2. The Norwegian Radium Hospital, Oslo, Norway

    Johan Moan

  3. University of Padua, Padua, Italy

    F. Dall’Acqua

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© 1988 Plenum Press, New York

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Brochon, JC., Livesey, A. (1988). Time-Resolved Fluorimetry using Synchrotron Radiation and Maximum Entropy Method of Analysis. In: Douglas, R.H., Moan, J., Dall’Acqua, F. (eds) Light in Biology and Medicine. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0709-9_3

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  • DOI: https://doi.org/10.1007/978-1-4613-0709-9_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8043-9

  • Online ISBN: 978-1-4613-0709-9

  • eBook Packages: Springer Book Archive

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