Nucleolar Changes in Hypotonic Treatment and the Possibility of Isolation of Nucleolar Fibrillar Complexes

  • P. Hozäk
  • O. V. Zatsepina
  • Z. Likovský


Nucleoli regularly contain the following components: the dense fibrillar component, fibrillar centers the granular component and the nucleolar chromatin (Schwarzacher and Wachtler, 1983; Goessens, 1984; Fakan and Hernandez-Verdun, 1986). Morphological studies suggest a parallel occurrence of the first two components: the fibrillar centers were never found in the active nucleoli without adjacent dense fibrillar component, and the fibrillar centers together with the dense fibrillar component (i.e. fibrillar complexes) seem to correspond to individual nucleolar organizers (Mirre and Stahl, 1981; Mirre and Kniebiehler, 1982; Hozák et al., 1986; Cataldo et al., 1988; Hozák et al., 1989).


Ehrlich Ascitic Tumor Cell Granular Component Dense Fibrillar Component Hypotonic Treatment Hypotonic Shock 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Cataldo C, Souchier C, and Stahl A. 1988, Three—dimensional ultrastructure and quantitative analysis of the human Sertoli cells nucleolus, Biol. Cell, 63:277.CrossRefGoogle Scholar
  2. Derenzini M., Farabegoli F., Pession A., and Novello F. 1987, Spatial redistribution of ribosomal chromatin in the fibrillar centers of human circulating lymphocytes after stimulation of transcription, Exp. Cell Res., 170:31.CrossRefGoogle Scholar
  3. Fakan S., and Hernandez-Verdun D, 1986, The nucleolus and the nucleolar organizer regions, Biol. Cell, 56:189.Google Scholar
  4. Goessens G., 1984, Nucleolar structure, in: ‘International review of cytology’, Academic Press, New York.Google Scholar
  5. Hozák P., Novák J. T., and Smetana K. 1989, Three—dimensional reconstructions of nucleolus-organizing regions in PHA—stimulated human lymphocytes, Biol. Cell, 66, in press.Google Scholar
  6. Hozák P., Zatsepina O., Vasilyeva I., and Chentsov Yu. 1986, An electron microscopic study of nucleolus — organizing regions at some stages of the cell cycle (G0 period, G2 period, mitosis), Biol. Cell, 57:197.Google Scholar
  7. Likovský Z., and Smetana K. 1972, Further studies on the cytochemistry of the standardized silver staining of interphase nucleoli in smear preparations of Yoshida ascitic sarcoma cells in rats, Histochemistry, 72:301.CrossRefGoogle Scholar
  8. Mirre C, and Kniebiehler B. 1982, A re-evaluation of the relationships between the fibrillar centers and the NORs in reticulated nucleoli: ultrastructural organization, number and distribution of the fibrillar centers in the nucleolus of mouse Sertoli cell, J. Cell Sci., 55:247.Google Scholar
  9. Mirre C. and Stahl A. 1981, Ultrastructural organization, sites of transcription and distribution of fibrillar centers in the nucleolus of the mouse oocyte, J. Cell Sci., 48:105.Google Scholar
  10. Scheer U., Messner K., Hazan R., Raska I., Hansmann P., Falk H., Spiess E., and Franke W. 1987, High sensitivity immunolocalization of double-end single—stranded DNA by a monoclonal antibody, Eur. J. Cell Biol., 43:358.Google Scholar
  11. Scheer U., and Rose K. M. 1984, Localization of RNA polymerase I in interphase cells and mitotic chromosome by light and electron micro-scopic immunocytochemistry, Proc. Natl. Acad. Sci. USA, 81:1431.CrossRefGoogle Scholar
  12. Schwarzacher H. G., and Wachtier F. 1983, Nucleolus organizer regions and nucleoli, Hum. Gen., 63:89.CrossRefGoogle Scholar
  13. Thiry M., Scheer U., and Goessens G. 1988a, Immunoelectron microscopic study of nucleolar DNA during mitosis in Ehrlich tumor cells, Eur. J. Cell Biol., 47:346.Google Scholar
  14. Thiry M., Scheer U., and Goessens G. 1988b, Localization of DNA within Ehrlich tumor cell nucleoli by immunoelectron microscopy, Biol. Cell, 63:27.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • P. Hozäk
    • 1
  • O. V. Zatsepina
    • 2
  • Z. Likovský
    • 1
  1. 1.Institute of Experimental MedicineCzechoslovak Academy of SciencesPrague 2Czechoslovakia
  2. 2.A. N. Belozersky Laboratory of Molecular Biology and Bioorganic ChemistryMoscow State UniversityMoscowUSSR

Personalised recommendations