Nucleolar Changes in Hypotonic Treatment and the Possibility of Isolation of Nucleolar Fibrillar Complexes

  • P. Hozäk
  • O. V. Zatsepina
  • Z. Likovský

Abstract

Nucleoli regularly contain the following components: the dense fibrillar component, fibrillar centers the granular component and the nucleolar chromatin (Schwarzacher and Wachtler, 1983; Goessens, 1984; Fakan and Hernandez-Verdun, 1986). Morphological studies suggest a parallel occurrence of the first two components: the fibrillar centers were never found in the active nucleoli without adjacent dense fibrillar component, and the fibrillar centers together with the dense fibrillar component (i.e. fibrillar complexes) seem to correspond to individual nucleolar organizers (Mirre and Stahl, 1981; Mirre and Kniebiehler, 1982; Hozák et al., 1986; Cataldo et al., 1988; Hozák et al., 1989).

Keywords

Formaldehyde Saccharose Fluoride MgCl2 Sarcoma 

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • P. Hozäk
    • 1
  • O. V. Zatsepina
    • 2
  • Z. Likovský
    • 1
  1. 1.Institute of Experimental MedicineCzechoslovak Academy of SciencesPrague 2Czechoslovakia
  2. 2.A. N. Belozersky Laboratory of Molecular Biology and Bioorganic ChemistryMoscow State UniversityMoscowUSSR

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