In Vitro Amplification of the Human Insulin Gene
DNA analysis is an increasingly important source of information in modern medicine. Various mutations responsible for genetic disorders have been described by means of direct analysis of human genomic DNA. Studies of a specific DNA sequence have to be preceded by construction and screening of the whole genome library to identify the sequences of interest. The alternative method is the polymerase chain reaction (PCR). PCR is an in vitro method for the primer-directed enzymatic amplification of specific DNA sequences using DNA polymerase (Saiki et al., 1985). With repeated cycles of DNA denaturation, annealing of the primers and DNA synthesis, a large number of copies of specific DNA sequences is faithfully synthesized in a few hours. The enzyme used in this procedure is a heat-stable DNA polymerase purified from bacterium Thermus aquaticus Taq DNA polymerase (Chien et al., 1976).
KeywordsAgarose Electrophoresis Polyacrylamide Proinsulin
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