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In Vitro Amplification of the Human Insulin Gene

  • Ryszard Slomski
  • Malgorzata Jungerman
  • Adam Kraszweski

Abstract

DNA analysis is an increasingly important source of information in modern medicine. Various mutations responsible for genetic disorders have been described by means of direct analysis of human genomic DNA. Studies of a specific DNA sequence have to be preceded by construction and screening of the whole genome library to identify the sequences of interest. The alternative method is the polymerase chain reaction (PCR). PCR is an in vitro method for the primer-directed enzymatic amplification of specific DNA sequences using DNA polymerase (Saiki et al., 1985). With repeated cycles of DNA denaturation, annealing of the primers and DNA synthesis, a large number of copies of specific DNA sequences is faithfully synthesized in a few hours. The enzyme used in this procedure is a heat-stable DNA polymerase purified from bacterium Thermus aquaticus Taq DNA polymerase (Chien et al., 1976).

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Product Human Insulin Insulin Gene Chain Code 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Ryszard Slomski
    • 1
  • Malgorzata Jungerman
    • 1
  • Adam Kraszweski
    • 2
  1. 1.Institute of Human GeneticsPolish Academy of SciencesPoznańPoland
  2. 2.Institute of Bioorganic ChemistryPolish Academy of SciencesPoznańPoland

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