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Molecular Genetic Analysis of Virulence Factors of Shigella Flexneri

  • Masanosuke Yoshikawa
  • Chihiro Sasakawa
  • Takashi Sakai
  • Masatoshi Yamada
  • Nobuhiko Okada
  • Sou-ichi Makino

Abstract

Labrec et al.7 established in 1964 that invasion into epithelial cells was the essential early step in the pathogenesis of bacillary dysentery. The virulent translucent strain of Shigella flexneri invaded the intestinal epithelium, while the avirulent opaque variant did not, in spite of the finding, that both had the same LD50 upon intraperitoneal injection. Ogawa et al.15 performed similar experiments and obtained essentially the same results. However, they made one important additional observation; within 24 hours of infection, the bacteria existed exclusively in the epithelial cells without direct penetration into the lamina propria and bacterial multiplication therein. Thus, virulent shigellae invade the colonic epithelium and spread successively to adjacent epithelial cells resulting in a severe inflammatory reaction, further spread of the bacteria to deeper tissue and, ultimately, to ulceration and bloody diarrhea.

Keywords

Corneal Epithelium Bacillary Dysentery Shigella Flexneri Adjacent Epithelial Cell Intergeneric Conjugation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Masanosuke Yoshikawa
    • 1
  • Chihiro Sasakawa
    • 1
  • Takashi Sakai
    • 1
  • Masatoshi Yamada
    • 1
  • Nobuhiko Okada
    • 1
  • Sou-ichi Makino
    • 1
  1. 1.Department of Bacteriology The Institute of Medical ScienceThe University of TokyoTokyoJapan

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