Abstract
A unique method to generate catalytic activity is described. The method consists of perturbation of a host protein, addition of a modifier, and crosslinking of the conformationally modified protein. The preparation and properties of three types of catalytic conformationally modified proteins (CCMP) are described. CCMP with amino acid esterase, glucose isomerase, and fluorohydrolase activities have been prepared from several host proteins, crosslinking reagents and modifiers. Although a fundamental understanding of this process is not known, the ease of preparation and unique properties of CCMP suggest that they will find numerous applications in biotechnology.
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© 1990 Plenum Press, New York
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Keyes, M.H., Albert, D.E. (1990). Generation of Catalytic Activity by Protein Modification. In: Gebelein, C.G. (eds) Biomimetic Polymers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0657-3_7
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DOI: https://doi.org/10.1007/978-1-4613-0657-3_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-7913-6
Online ISBN: 978-1-4613-0657-3
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